Amino acid dipepetide frequency for Lactococcus reticulitermitis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.725AlaAla: 6.725 ± 0.162
0.461AlaCys: 0.461 ± 0.027
5.273AlaAsp: 5.273 ± 0.092
5.442AlaGlu: 5.442 ± 0.114
3.482AlaPhe: 3.482 ± 0.073
5.856AlaGly: 5.856 ± 0.109
1.425AlaHis: 1.425 ± 0.049
6.479AlaIle: 6.479 ± 0.115
5.78AlaLys: 5.78 ± 0.118
7.946AlaLeu: 7.946 ± 0.126
2.127AlaMet: 2.127 ± 0.058
3.706AlaAsn: 3.706 ± 0.082
2.44AlaPro: 2.44 ± 0.074
3.448AlaGln: 3.448 ± 0.087
3.004AlaArg: 3.004 ± 0.058
4.67AlaSer: 4.67 ± 0.1
5.484AlaThr: 5.484 ± 0.135
5.816AlaVal: 5.816 ± 0.096
0.668AlaTrp: 0.668 ± 0.034
2.901AlaTyr: 2.901 ± 0.071
0.0AlaXaa: 0.0 ± 0.0
Cys
0.321CysAla: 0.321 ± 0.022
0.065CysCys: 0.065 ± 0.011
0.279CysAsp: 0.279 ± 0.021
0.254CysGlu: 0.254 ± 0.021
0.249CysPhe: 0.249 ± 0.022
0.476CysGly: 0.476 ± 0.029
0.142CysHis: 0.142 ± 0.017
0.279CysIle: 0.279 ± 0.021
0.196CysLys: 0.196 ± 0.018
0.522CysLeu: 0.522 ± 0.031
0.106CysMet: 0.106 ± 0.013
0.176CysAsn: 0.176 ± 0.016
0.23CysPro: 0.23 ± 0.022
0.249CysGln: 0.249 ± 0.019
0.179CysArg: 0.179 ± 0.017
0.29CysSer: 0.29 ± 0.021
0.24CysThr: 0.24 ± 0.02
0.304CysVal: 0.304 ± 0.024
0.047CysTrp: 0.047 ± 0.008
0.171CysTyr: 0.171 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
4.636AspAla: 4.636 ± 0.098
0.291AspCys: 0.291 ± 0.022
3.533AspAsp: 3.533 ± 0.084
4.263AspGlu: 4.263 ± 0.096
3.585AspPhe: 3.585 ± 0.078
4.13AspGly: 4.13 ± 0.097
0.973AspHis: 0.973 ± 0.047
4.481AspIle: 4.481 ± 0.087
4.377AspLys: 4.377 ± 0.093
6.059AspLeu: 6.059 ± 0.111
1.612AspMet: 1.612 ± 0.051
2.733AspAsn: 2.733 ± 0.07
1.548AspPro: 1.548 ± 0.052
1.85AspGln: 1.85 ± 0.055
1.892AspArg: 1.892 ± 0.063
2.806AspSer: 2.806 ± 0.06
3.258AspThr: 3.258 ± 0.087
4.401AspVal: 4.401 ± 0.088
0.687AspTrp: 0.687 ± 0.035
2.759AspTyr: 2.759 ± 0.073
0.0AspXaa: 0.0 ± 0.0
Glu
5.1GluAla: 5.1 ± 0.11
0.229GluCys: 0.229 ± 0.017
2.878GluAsp: 2.878 ± 0.077
3.469GluGlu: 3.469 ± 0.097
2.596GluPhe: 2.596 ± 0.068
3.038GluGly: 3.038 ± 0.073
1.123GluHis: 1.123 ± 0.041
5.61GluIle: 5.61 ± 0.09
5.737GluLys: 5.737 ± 0.098
6.241GluLeu: 6.241 ± 0.112
1.892GluMet: 1.892 ± 0.057
3.997GluAsn: 3.997 ± 0.084
1.587GluPro: 1.587 ± 0.051
2.474GluGln: 2.474 ± 0.074
2.583GluArg: 2.583 ± 0.072
2.96GluSer: 2.96 ± 0.074
4.371GluThr: 4.371 ± 0.093
4.416GluVal: 4.416 ± 0.091
0.448GluTrp: 0.448 ± 0.028
1.599GluTyr: 1.599 ± 0.054
0.0GluXaa: 0.0 ± 0.0
Phe
3.483PheAla: 3.483 ± 0.08
0.249PheCys: 0.249 ± 0.02
3.53PheAsp: 3.53 ± 0.086
3.284PheGlu: 3.284 ± 0.075
1.959PhePhe: 1.959 ± 0.063
3.412PheGly: 3.412 ± 0.085
0.69PheHis: 0.69 ± 0.033
3.342PheIle: 3.342 ± 0.077
2.611PheLys: 2.611 ± 0.071
4.123PheLeu: 4.123 ± 0.103
1.116PheMet: 1.116 ± 0.045
2.06PheAsn: 2.06 ± 0.06
1.568PhePro: 1.568 ± 0.058
1.524PheGln: 1.524 ± 0.052
1.454PheArg: 1.454 ± 0.047
3.346PheSer: 3.346 ± 0.077
2.577PheThr: 2.577 ± 0.067
3.191PheVal: 3.191 ± 0.078
0.48PheTrp: 0.48 ± 0.034
1.691PheTyr: 1.691 ± 0.056
0.0PheXaa: 0.0 ± 0.0
Gly
5.293GlyAla: 5.293 ± 0.112
0.38GlyCys: 0.38 ± 0.024
3.761GlyAsp: 3.761 ± 0.092
3.936GlyGlu: 3.936 ± 0.077
3.357GlyPhe: 3.357 ± 0.081
4.824GlyGly: 4.824 ± 0.148
1.28GlyHis: 1.28 ± 0.045
5.282GlyIle: 5.282 ± 0.115
4.724GlyLys: 4.724 ± 0.094
6.249GlyLeu: 6.249 ± 0.107
1.806GlyMet: 1.806 ± 0.065
2.979GlyAsn: 2.979 ± 0.081
1.204GlyPro: 1.204 ± 0.043
2.955GlyGln: 2.955 ± 0.069
2.557GlyArg: 2.557 ± 0.069
3.75GlySer: 3.75 ± 0.1
4.43GlyThr: 4.43 ± 0.123
5.114GlyVal: 5.114 ± 0.097
0.687GlyTrp: 0.687 ± 0.04
2.681GlyTyr: 2.681 ± 0.082
0.0GlyXaa: 0.0 ± 0.0
His
1.389HisAla: 1.389 ± 0.054
0.107HisCys: 0.107 ± 0.013
1.141HisAsp: 1.141 ± 0.04
1.081HisGlu: 1.081 ± 0.043
1.101HisPhe: 1.101 ± 0.047
1.303HisGly: 1.303 ± 0.044
0.576HisHis: 0.576 ± 0.032
1.241HisIle: 1.241 ± 0.046
0.866HisLys: 0.866 ± 0.035
1.965HisLeu: 1.965 ± 0.062
0.339HisMet: 0.339 ± 0.022
0.713HisAsn: 0.713 ± 0.027
0.855HisPro: 0.855 ± 0.034
0.939HisGln: 0.939 ± 0.044
0.761HisArg: 0.761 ± 0.032
0.863HisSer: 0.863 ± 0.047
0.934HisThr: 0.934 ± 0.044
1.235HisVal: 1.235 ± 0.047
0.165HisTrp: 0.165 ± 0.015
0.775HisTyr: 0.775 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
6.753IleAla: 6.753 ± 0.107
0.498IleCys: 0.498 ± 0.031
4.611IleAsp: 4.611 ± 0.082
4.626IleGlu: 4.626 ± 0.098
3.591IlePhe: 3.591 ± 0.086
5.227IleGly: 5.227 ± 0.108
1.275IleHis: 1.275 ± 0.048
5.302IleIle: 5.302 ± 0.115
4.946IleLys: 4.946 ± 0.097
7.315IleLeu: 7.315 ± 0.137
1.601IleMet: 1.601 ± 0.054
3.404IleAsn: 3.404 ± 0.081
2.759IlePro: 2.759 ± 0.073
2.524IleGln: 2.524 ± 0.07
2.624IleArg: 2.624 ± 0.064
5.165IleSer: 5.165 ± 0.098
4.511IleThr: 4.511 ± 0.084
5.109IleVal: 5.109 ± 0.09
0.564IleTrp: 0.564 ± 0.027
2.523IleTyr: 2.523 ± 0.07
0.0IleXaa: 0.0 ± 0.0
Lys
5.688LysAla: 5.688 ± 0.117
0.215LysCys: 0.215 ± 0.021
3.53LysAsp: 3.53 ± 0.082
4.119LysGlu: 4.119 ± 0.102
2.638LysPhe: 2.638 ± 0.066
3.589LysGly: 3.589 ± 0.073
1.112LysHis: 1.112 ± 0.041
5.662LysIle: 5.662 ± 0.106
5.481LysLys: 5.481 ± 0.116
6.201LysLeu: 6.201 ± 0.109
2.15LysMet: 2.15 ± 0.068
3.908LysAsn: 3.908 ± 0.08
2.046LysPro: 2.046 ± 0.061
2.509LysGln: 2.509 ± 0.064
2.826LysArg: 2.826 ± 0.07
4.005LysSer: 4.005 ± 0.08
4.461LysThr: 4.461 ± 0.088
4.566LysVal: 4.566 ± 0.1
0.589LysTrp: 0.589 ± 0.029
2.284LysTyr: 2.284 ± 0.062
0.0LysXaa: 0.0 ± 0.0
Leu
9.022LeuAla: 9.022 ± 0.142
0.428LeuCys: 0.428 ± 0.027
5.888LeuAsp: 5.888 ± 0.123
5.832LeuGlu: 5.832 ± 0.106
3.989LeuPhe: 3.989 ± 0.094
6.236LeuGly: 6.236 ± 0.127
1.495LeuHis: 1.495 ± 0.054
6.802LeuIle: 6.802 ± 0.125
6.168LeuLys: 6.168 ± 0.109
8.813LeuLeu: 8.813 ± 0.158
2.491LeuMet: 2.491 ± 0.064
4.318LeuAsn: 4.318 ± 0.08
4.072LeuPro: 4.072 ± 0.091
3.189LeuGln: 3.189 ± 0.071
3.311LeuArg: 3.311 ± 0.077
7.032LeuSer: 7.032 ± 0.104
7.004LeuThr: 7.004 ± 0.1
6.496LeuVal: 6.496 ± 0.107
0.663LeuTrp: 0.663 ± 0.035
2.801LeuTyr: 2.801 ± 0.07
0.0LeuXaa: 0.0 ± 0.0
Met
2.305MetAla: 2.305 ± 0.06
0.097MetCys: 0.097 ± 0.013
1.183MetAsp: 1.183 ± 0.043
0.936MetGlu: 0.936 ± 0.039
0.827MetPhe: 0.827 ± 0.04
1.602MetGly: 1.602 ± 0.053
0.355MetHis: 0.355 ± 0.023
1.999MetIle: 1.999 ± 0.059
1.876MetLys: 1.876 ± 0.055
2.206MetLeu: 2.206 ± 0.066
0.797MetMet: 0.797 ± 0.043
1.204MetAsn: 1.204 ± 0.042
1.082MetPro: 1.082 ± 0.046
0.941MetGln: 0.941 ± 0.04
1.034MetArg: 1.034 ± 0.041
1.733MetSer: 1.733 ± 0.056
2.549MetThr: 2.549 ± 0.071
1.68MetVal: 1.68 ± 0.052
0.14MetTrp: 0.14 ± 0.016
0.545MetTyr: 0.545 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.611AsnAla: 3.611 ± 0.084
0.223AsnCys: 0.223 ± 0.018
2.597AsnAsp: 2.597 ± 0.06
2.393AsnGlu: 2.393 ± 0.065
2.329AsnPhe: 2.329 ± 0.067
3.857AsnGly: 3.857 ± 0.1
1.092AsnHis: 1.092 ± 0.049
3.429AsnIle: 3.429 ± 0.084
2.694AsnLys: 2.694 ± 0.067
4.863AsnLeu: 4.863 ± 0.086
1.115AsnMet: 1.115 ± 0.046
1.917AsnAsn: 1.917 ± 0.066
2.294AsnPro: 2.294 ± 0.066
2.361AsnGln: 2.361 ± 0.061
1.837AsnArg: 1.837 ± 0.066
2.417AsnSer: 2.417 ± 0.067
2.347AsnThr: 2.347 ± 0.078
3.102AsnVal: 3.102 ± 0.067
0.498AsnTrp: 0.498 ± 0.029
1.816AsnTyr: 1.816 ± 0.054
0.0AsnXaa: 0.0 ± 0.0
Pro
2.484ProAla: 2.484 ± 0.075
0.137ProCys: 0.137 ± 0.015
2.448ProAsp: 2.448 ± 0.067
3.089ProGlu: 3.089 ± 0.081
1.647ProPhe: 1.647 ± 0.056
1.879ProGly: 1.879 ± 0.062
0.629ProHis: 0.629 ± 0.036
2.544ProIle: 2.544 ± 0.065
2.188ProLys: 2.188 ± 0.055
2.868ProLeu: 2.868 ± 0.073
0.772ProMet: 0.772 ± 0.032
1.605ProAsn: 1.605 ± 0.057
0.631ProPro: 0.631 ± 0.038
1.269ProGln: 1.269 ± 0.052
0.99ProArg: 0.99 ± 0.038
2.079ProSer: 2.079 ± 0.059
2.308ProThr: 2.308 ± 0.084
2.56ProVal: 2.56 ± 0.061
0.302ProTrp: 0.302 ± 0.021
1.191ProTyr: 1.191 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
4.159GlnAla: 4.159 ± 0.09
0.101GlnCys: 0.101 ± 0.012
2.057GlnAsp: 2.057 ± 0.052
2.546GlnGlu: 2.546 ± 0.076
1.582GlnPhe: 1.582 ± 0.052
2.241GlnGly: 2.241 ± 0.055
0.788GlnHis: 0.788 ± 0.038
3.113GlnIle: 3.113 ± 0.067
2.907GlnLys: 2.907 ± 0.071
3.728GlnLeu: 3.728 ± 0.088
0.987GlnMet: 0.987 ± 0.042
1.999GlnAsn: 1.999 ± 0.056
1.282GlnPro: 1.282 ± 0.045
1.517GlnGln: 1.517 ± 0.056
1.341GlnArg: 1.341 ± 0.05
2.094GlnSer: 2.094 ± 0.065
2.772GlnThr: 2.772 ± 0.079
2.943GlnVal: 2.943 ± 0.072
0.29GlnTrp: 0.29 ± 0.022
1.255GlnTyr: 1.255 ± 0.046
0.0GlnXaa: 0.0 ± 0.0
Arg
2.652ArgAla: 2.652 ± 0.07
0.153ArgCys: 0.153 ± 0.016
2.27ArgAsp: 2.27 ± 0.064
2.762ArgGlu: 2.762 ± 0.06
1.827ArgPhe: 1.827 ± 0.06
2.164ArgGly: 2.164 ± 0.062
0.788ArgHis: 0.788 ± 0.04
2.656ArgIle: 2.656 ± 0.063
2.509ArgLys: 2.509 ± 0.072
3.72ArgLeu: 3.72 ± 0.092
0.975ArgMet: 0.975 ± 0.035
1.568ArgAsn: 1.568 ± 0.054
1.143ArgPro: 1.143 ± 0.036
1.96ArgGln: 1.96 ± 0.062
1.7ArgArg: 1.7 ± 0.054
1.755ArgSer: 1.755 ± 0.053
1.886ArgThr: 1.886 ± 0.057
2.504ArgVal: 2.504 ± 0.07
0.266ArgTrp: 0.266 ± 0.02
1.429ArgTyr: 1.429 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
4.413SerAla: 4.413 ± 0.113
0.268SerCys: 0.268 ± 0.023
4.148SerAsp: 4.148 ± 0.071
4.137SerGlu: 4.137 ± 0.082
2.72SerPhe: 2.72 ± 0.068
4.861SerGly: 4.861 ± 0.109
1.215SerHis: 1.215 ± 0.041
3.982SerIle: 3.982 ± 0.089
3.799SerLys: 3.799 ± 0.092
5.821SerLeu: 5.821 ± 0.099
1.19SerMet: 1.19 ± 0.049
2.733SerAsn: 2.733 ± 0.085
1.806SerPro: 1.806 ± 0.06
2.656SerGln: 2.656 ± 0.069
2.216SerArg: 2.216 ± 0.059
3.745SerSer: 3.745 ± 0.109
3.172SerThr: 3.172 ± 0.086
4.088SerVal: 4.088 ± 0.084
0.568SerTrp: 0.568 ± 0.033
2.094SerTyr: 2.094 ± 0.066
0.0SerXaa: 0.0 ± 0.0
Thr
5.361ThrAla: 5.361 ± 0.118
0.227ThrCys: 0.227 ± 0.017
3.798ThrAsp: 3.798 ± 0.096
3.628ThrGlu: 3.628 ± 0.078
2.969ThrPhe: 2.969 ± 0.061
5.078ThrGly: 5.078 ± 0.12
1.28ThrHis: 1.28 ± 0.045
4.832ThrIle: 4.832 ± 0.095
3.634ThrLys: 3.634 ± 0.079
6.303ThrLeu: 6.303 ± 0.099
1.241ThrMet: 1.241 ± 0.044
2.655ThrAsn: 2.655 ± 0.074
2.935ThrPro: 2.935 ± 0.105
2.395ThrGln: 2.395 ± 0.063
1.992ThrArg: 1.992 ± 0.05
3.922ThrSer: 3.922 ± 0.103
4.204ThrThr: 4.204 ± 0.124
4.935ThrVal: 4.935 ± 0.104
0.582ThrTrp: 0.582 ± 0.032
2.467ThrTyr: 2.467 ± 0.083
0.0ThrXaa: 0.0 ± 0.0
Val
6.479ValAla: 6.479 ± 0.115
0.367ValCys: 0.367 ± 0.028
3.919ValAsp: 3.919 ± 0.071
3.985ValGlu: 3.985 ± 0.081
2.962ValPhe: 2.962 ± 0.08
4.558ValGly: 4.558 ± 0.087
1.081ValHis: 1.081 ± 0.04
5.339ValIle: 5.339 ± 0.102
4.879ValLys: 4.879 ± 0.106
6.464ValLeu: 6.464 ± 0.106
1.867ValMet: 1.867 ± 0.056
3.194ValAsn: 3.194 ± 0.079
2.625ValPro: 2.625 ± 0.071
2.244ValGln: 2.244 ± 0.06
2.424ValArg: 2.424 ± 0.077
4.631ValSer: 4.631 ± 0.088
5.241ValThr: 5.241 ± 0.13
5.137ValVal: 5.137 ± 0.088
0.562ValTrp: 0.562 ± 0.032
2.319ValTyr: 2.319 ± 0.073
0.0ValXaa: 0.0 ± 0.0
Trp
0.567TrpAla: 0.567 ± 0.035
0.07TrpCys: 0.07 ± 0.009
0.5TrpAsp: 0.5 ± 0.029
0.517TrpGlu: 0.517 ± 0.03
0.43TrpPhe: 0.43 ± 0.028
0.615TrpGly: 0.615 ± 0.034
0.23TrpHis: 0.23 ± 0.019
0.442TrpIle: 0.442 ± 0.027
0.383TrpLys: 0.383 ± 0.029
1.07TrpLeu: 1.07 ± 0.043
0.196TrpMet: 0.196 ± 0.016
0.392TrpAsn: 0.392 ± 0.025
0.188TrpPro: 0.188 ± 0.017
0.624TrpGln: 0.624 ± 0.03
0.431TrpArg: 0.431 ± 0.028
0.543TrpSer: 0.543 ± 0.035
0.519TrpThr: 0.519 ± 0.031
0.491TrpVal: 0.491 ± 0.028
0.126TrpTrp: 0.126 ± 0.014
0.336TrpTyr: 0.336 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.692TyrAla: 2.692 ± 0.071
0.207TyrCys: 0.207 ± 0.018
2.529TyrAsp: 2.529 ± 0.073
2.048TyrGlu: 2.048 ± 0.062
1.851TyrPhe: 1.851 ± 0.062
2.3TyrGly: 2.3 ± 0.064
0.803TyrHis: 0.803 ± 0.03
2.111TyrIle: 2.111 ± 0.064
1.823TyrLys: 1.823 ± 0.058
3.574TyrLeu: 3.574 ± 0.078
0.709TyrMet: 0.709 ± 0.029
1.531TyrAsn: 1.531 ± 0.054
1.327TyrPro: 1.327 ± 0.053
2.073TyrGln: 2.073 ± 0.057
1.531TyrArg: 1.531 ± 0.047
1.827TyrSer: 1.827 ± 0.058
2.172TyrThr: 2.172 ± 0.084
2.192TyrVal: 2.192 ± 0.062
0.339TyrTrp: 0.339 ± 0.02
1.468TyrTyr: 1.468 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2097 proteins (642200 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski