Pacificibacter maritimus
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2932 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N4ULS1|A0A3N4ULS1_9RHOB Uncharacterized protein OS=Pacificibacter maritimus OX=762213 GN=EDD53_0501 PE=4 SV=1
MM1 pKa = 7.48 NKK3 pKa = 9.65 PLSRR7 pKa = 11.84 LSKK10 pKa = 9.78 SAINISLSIFLTGFAPVSPVAAGEE34 pKa = 4.15 FQVDD38 pKa = 3.2 WGTFDD43 pKa = 3.12 WPAGFTGPVTRR54 pKa = 11.84 TWSDD58 pKa = 3.22 QYY60 pKa = 11.6 GFEE63 pKa = 4.19 IDD65 pKa = 4.1 VEE67 pKa = 4.47 AEE69 pKa = 3.82 LSGTFQSYY77 pKa = 10.66 VDD79 pKa = 3.53 GTGAVVPSPDD89 pKa = 3.61 DD90 pKa = 3.59 VDD92 pKa = 4.7 FFGGNVEE99 pKa = 4.24 SLVVVGDD106 pKa = 3.65 ARR108 pKa = 11.84 ANEE111 pKa = 4.31 GAFGDD116 pKa = 3.8 NRR118 pKa = 11.84 ITATVRR124 pKa = 11.84 AQSGGVAFPVDD135 pKa = 3.6 SLEE138 pKa = 4.84 IDD140 pKa = 3.73 VLDD143 pKa = 5.31 IDD145 pKa = 4.16 STDD148 pKa = 3.25 NNSRR152 pKa = 11.84 EE153 pKa = 4.08 DD154 pKa = 3.2 RR155 pKa = 11.84 CDD157 pKa = 3.28 FVTGFGNNGNPTFSTLSATPSVLVGPGPGSGLTGTIGANQAQCIYY202 pKa = 10.47 IEE204 pKa = 4.52 GATVSPTSPNDD215 pKa = 3.24 STGSVRR221 pKa = 11.84 ATFPNGTSEE230 pKa = 3.84 ATFWYY235 pKa = 10.03 DD236 pKa = 3.63 EE237 pKa = 4.52 SIQNVRR243 pKa = 11.84 NYY245 pKa = 11.14 SVFSSYY251 pKa = 11.48 NPGARR256 pKa = 11.84 GIGMFGNVGFTVDD269 pKa = 3.52 QSINLTRR276 pKa = 11.84 SVSPNSAIEE285 pKa = 4.2 ADD287 pKa = 4.32 TITYY291 pKa = 8.69 TYY293 pKa = 10.98 VVEE296 pKa = 4.28 NTGALPFNPNQDD308 pKa = 3.78 MIIEE312 pKa = 4.57 DD313 pKa = 4.1 SLLGTVTCPAITAPIAPGGTVTCTGSYY340 pKa = 9.24 TVTAADD346 pKa = 3.72 VLAGAVDD353 pKa = 3.9 STATAGIGAIGQPFVSRR370 pKa = 11.84 LQSNTEE376 pKa = 3.77 NASLVTSSLLGTTDD390 pKa = 3.52 PQICTPEE397 pKa = 3.93 NVFAKK402 pKa = 10.31 PRR404 pKa = 11.84 TQLAGSGNSFDD415 pKa = 3.88 VQVGDD420 pKa = 4.5 IYY422 pKa = 11.42 LFDD425 pKa = 4.48 NVTTDD430 pKa = 3.35 TNGNALDD437 pKa = 3.73 MVVEE441 pKa = 4.62 FTEE444 pKa = 4.35 VSNATSIVLDD454 pKa = 3.69 TALDD458 pKa = 3.54 ARR460 pKa = 11.84 MTPSEE465 pKa = 4.24 NGYY468 pKa = 10.06 VIYY471 pKa = 10.46 NVRR474 pKa = 11.84 LVQDD478 pKa = 4.03 GSAVPSAPLGTLVEE492 pKa = 4.1 QSRR495 pKa = 11.84 ISGVIAQHH503 pKa = 6.74 LDD505 pKa = 2.88 VDD507 pKa = 4.57 SRR509 pKa = 11.84 GSAQDD514 pKa = 3.22 STDD517 pKa = 3.17 VVGFLDD523 pKa = 4.03 GNPVVSLFNTAPLASYY539 pKa = 10.13 PSGGSLYY546 pKa = 10.74 TMDD549 pKa = 4.51 PAKK552 pKa = 10.71 VGNPLDD558 pKa = 3.52 WLEE561 pKa = 4.32 EE562 pKa = 4.22 ANEE565 pKa = 4.35 SAFDD569 pKa = 3.54 NYY571 pKa = 8.09 VTYY574 pKa = 10.03 EE575 pKa = 4.05 YY576 pKa = 10.52 STFTQEE582 pKa = 4.73 RR583 pKa = 11.84 FIHH586 pKa = 6.94 GYY588 pKa = 8.33 TGTSTTAATRR598 pKa = 11.84 GTSFTFCAISNTSATVVAEE617 pKa = 4.39 DD618 pKa = 4.68 DD619 pKa = 5.22 DD620 pKa = 4.51 YY621 pKa = 11.92 TATPVNTLLGGTAGEE636 pKa = 4.28 VLANDD641 pKa = 4.22 TVNGLPATSGNVTLEE656 pKa = 4.22 VLTAAQPVNSDD667 pKa = 3.59 DD668 pKa = 3.68 PVPYY672 pKa = 10.33 LVEE675 pKa = 4.27 TGLDD679 pKa = 3.25 AGRR682 pKa = 11.84 VIVPEE687 pKa = 3.96 NVPSGTYY694 pKa = 10.03 QIDD697 pKa = 3.7 YY698 pKa = 8.44 EE699 pKa = 4.34 ICDD702 pKa = 3.52 VLDD705 pKa = 4.13 PLQCDD710 pKa = 3.29 RR711 pKa = 11.84 AKK713 pKa = 11.14 VLVAVFDD720 pKa = 4.01 GLGVDD725 pKa = 5.91 FSDD728 pKa = 4.9 APISYY733 pKa = 9.96 LVAAHH738 pKa = 6.68 GVDD741 pKa = 3.95 TVPSVYY747 pKa = 10.3 LGSVPPDD754 pKa = 3.1 IEE756 pKa = 6.81 AIAQSDD762 pKa = 3.68 ATATADD768 pKa = 4.69 DD769 pKa = 4.86 LLGTDD774 pKa = 5.22 DD775 pKa = 4.75 EE776 pKa = 5.32 DD777 pKa = 4.68 SVMFPILTQGTISTVDD793 pKa = 3.07 VGVTGNGFLQAWIDD807 pKa = 3.85 FNGDD811 pKa = 3.33 GLFEE815 pKa = 4.16 EE816 pKa = 4.9 TLGEE820 pKa = 4.49 RR821 pKa = 11.84 IAIDD825 pKa = 3.65 LQDD828 pKa = 4.07 NGTGADD834 pKa = 3.56 NLAGDD839 pKa = 4.49 GVIQITVTVPSDD851 pKa = 3.09 ATTSTTFARR860 pKa = 11.84 FRR862 pKa = 11.84 YY863 pKa = 9.65 SSEE866 pKa = 3.57 TGLPVSSFAVDD877 pKa = 4.28 GEE879 pKa = 4.48 VEE881 pKa = 4.56 DD882 pKa = 4.29 YY883 pKa = 11.33 SLVIAEE889 pKa = 4.45 TDD891 pKa = 3.41 LVDD894 pKa = 5.44 RR895 pKa = 11.84 GDD897 pKa = 3.93 APASYY902 pKa = 10.51 GDD904 pKa = 3.63 PRR906 pKa = 11.84 HH907 pKa = 6.06 IVVPSIYY914 pKa = 10.26 LGAGLPDD921 pKa = 3.79 TEE923 pKa = 5.04 VLTQHH928 pKa = 6.73 SSGADD933 pKa = 3.33 ADD935 pKa = 4.37 DD936 pKa = 5.19 LSGLDD941 pKa = 4.65 DD942 pKa = 4.4 EE943 pKa = 6.1 DD944 pKa = 5.93 SIAVFPVLEE953 pKa = 4.72 AGTTVSLTVQTHH965 pKa = 4.49 EE966 pKa = 4.54 TLSNLYY972 pKa = 10.26 DD973 pKa = 3.93 AGLPVLSPGVTNLQLWIDD991 pKa = 3.77 WDD993 pKa = 3.61 QSGTFEE999 pKa = 4.18 TSEE1002 pKa = 3.98 QVAIDD1007 pKa = 3.9 YY1008 pKa = 10.65 RR1009 pKa = 11.84 DD1010 pKa = 3.57 GGAGDD1015 pKa = 4.0 ADD1017 pKa = 3.52 GVFNNQITMNIAVPNTIVSGLTFARR1042 pKa = 11.84 VRR1044 pKa = 11.84 WSTSSAVATDD1054 pKa = 4.07 PFDD1057 pKa = 3.75 GLNFDD1062 pKa = 5.4 GEE1064 pKa = 4.55 VEE1066 pKa = 4.33 DD1067 pKa = 4.07 YY1068 pKa = 10.43 QVRR1071 pKa = 11.84 LSAGAVPFLCDD1082 pKa = 3.03 GTLYY1086 pKa = 10.2 RR1087 pKa = 11.84 VAGISSQLQEE1097 pKa = 5.0 LVFTEE1102 pKa = 4.38 NGAGGYY1108 pKa = 8.22 TIADD1112 pKa = 3.68 ATVGTPAGVDD1122 pKa = 3.58 YY1123 pKa = 11.05 NAAWGYY1129 pKa = 10.13 NALDD1133 pKa = 3.39 GLFYY1137 pKa = 11.05 GVVNGTRR1144 pKa = 11.84 DD1145 pKa = 3.61 LVRR1148 pKa = 11.84 VDD1150 pKa = 3.23 SSGNFTVISTIPTTAAIGDD1169 pKa = 3.92 GAGDD1173 pKa = 3.29 IMEE1176 pKa = 4.67 NGIMVYY1182 pKa = 10.08 RR1183 pKa = 11.84 VNSGNAFQLLDD1194 pKa = 4.22 LSNPFAPLDD1203 pKa = 3.98 LGQLTFSAPLSVADD1217 pKa = 4.22 IAYY1220 pKa = 10.04 NPNDD1224 pKa = 3.26 GFFYY1228 pKa = 10.62 GINATTNRR1236 pKa = 11.84 LFYY1239 pKa = 10.4 FDD1241 pKa = 5.05 PLAGTAGATSIVEE1254 pKa = 4.17 FGPATFTADD1263 pKa = 3.45 YY1264 pKa = 9.72 GAVWFDD1270 pKa = 3.38 YY1271 pKa = 10.96 YY1272 pKa = 11.54 GRR1274 pKa = 11.84 FYY1276 pKa = 10.85 INQNNTNEE1284 pKa = 4.04 VFQVDD1289 pKa = 3.64 VGFEE1293 pKa = 4.11 GDD1295 pKa = 3.27 GDD1297 pKa = 4.05 GAATVIDD1304 pKa = 4.38 TLTLPSEE1311 pKa = 4.29 NRR1313 pKa = 11.84 VNAASCPSRR1322 pKa = 11.84 FGPLPPEE1329 pKa = 4.55 GSVQGLVYY1337 pKa = 10.68 NDD1339 pKa = 4.2 SNLSGTPDD1347 pKa = 3.42 SPSEE1351 pKa = 3.91 TGIAGITVSAYY1362 pKa = 10.73 DD1363 pKa = 3.75 NNSTPEE1369 pKa = 3.99 NLSDD1373 pKa = 3.83 DD1374 pKa = 4.22 TLVAATQTDD1383 pKa = 3.67 SLGQFTLEE1391 pKa = 3.97 GLSALRR1397 pKa = 11.84 VYY1399 pKa = 10.49 RR1400 pKa = 11.84 IEE1402 pKa = 4.05 VDD1404 pKa = 3.42 EE1405 pKa = 5.3 ADD1407 pKa = 3.63 EE1408 pKa = 4.68 DD1409 pKa = 4.34 LPIGAQITSEE1419 pKa = 3.87 NPYY1422 pKa = 9.86 PNVRR1426 pKa = 11.84 VTAGSRR1432 pKa = 11.84 SSGYY1436 pKa = 10.87 DD1437 pKa = 2.95 FGFASTADD1445 pKa = 3.62 LSITKK1450 pKa = 9.8 VALDD1454 pKa = 3.81 TNGVPLTEE1462 pKa = 4.19 AQEE1465 pKa = 4.16 GTEE1468 pKa = 3.61 IDD1470 pKa = 3.87 FNLTVTNDD1478 pKa = 3.02 GTNAVTGVKK1487 pKa = 10.11 VRR1489 pKa = 11.84 DD1490 pKa = 3.58 LLPNGFSYY1498 pKa = 11.35 VSDD1501 pKa = 3.47 TAAASGDD1508 pKa = 3.89 TYY1510 pKa = 11.82 DD1511 pKa = 4.97 LGTGVWDD1518 pKa = 4.2 IGDD1521 pKa = 4.24 LAAGSSISLTIRR1533 pKa = 11.84 ATMNATGEE1541 pKa = 4.27 HH1542 pKa = 5.75 TNVAEE1547 pKa = 5.17 IIASSLNDD1555 pKa = 4.07 PDD1557 pKa = 4.17 SDD1559 pKa = 4.11 PAVGPLTDD1567 pKa = 4.88 DD1568 pKa = 4.2 LSDD1571 pKa = 4.94 GIADD1575 pKa = 4.12 DD1576 pKa = 5.53 DD1577 pKa = 4.26 EE1578 pKa = 7.05 ASVTVTFVGGGSVLSGTVFQDD1599 pKa = 2.92 NGAGSGTAFDD1609 pKa = 4.47 GVQNGLEE1616 pKa = 4.2 VGTPAAKK1623 pKa = 10.53 VEE1625 pKa = 4.22 VFNGAGTLIDD1635 pKa = 4.15 SPEE1638 pKa = 3.95 VAADD1642 pKa = 3.97 GTWSLTLPDD1651 pKa = 4.23 SFAGPVTLQVTAAATHH1667 pKa = 5.96 LVISEE1672 pKa = 4.35 TPAALPFGVNTDD1684 pKa = 3.28 PRR1686 pKa = 11.84 DD1687 pKa = 3.5 GRR1689 pKa = 11.84 FTFTPDD1695 pKa = 3.42 PDD1697 pKa = 3.32 EE1698 pKa = 5.9 DD1699 pKa = 4.32 YY1700 pKa = 11.54 AGLDD1704 pKa = 3.36 VGVLKK1709 pKa = 10.21 QARR1712 pKa = 11.84 LRR1714 pKa = 11.84 EE1715 pKa = 4.29 DD1716 pKa = 3.05 QSGVIGAGQVAEE1728 pKa = 4.57 LRR1730 pKa = 11.84 HH1731 pKa = 5.57 EE1732 pKa = 4.91 FIVDD1736 pKa = 3.48 AEE1738 pKa = 4.42 GSVAFSVTPLSEE1750 pKa = 4.37 TPAGGYY1756 pKa = 8.93 SHH1758 pKa = 7.33 ALFEE1762 pKa = 4.93 DD1763 pKa = 4.45 TNCDD1767 pKa = 3.0 GTADD1771 pKa = 4.0 TVISGALTTSPDD1783 pKa = 3.46 QLICLVLRR1791 pKa = 11.84 VSASSSIGPNASYY1804 pKa = 10.83 VVQLDD1809 pKa = 4.4 AITTYY1814 pKa = 11.0 GATGVSEE1821 pKa = 4.9 LDD1823 pKa = 3.5 RR1824 pKa = 11.84 NTDD1827 pKa = 3.23 RR1828 pKa = 11.84 LTTEE1832 pKa = 4.08 ASTGALRR1839 pKa = 11.84 LYY1841 pKa = 9.55 KK1842 pKa = 9.35 TVRR1845 pKa = 11.84 NITQGGAEE1853 pKa = 4.17 GVRR1856 pKa = 11.84 NGGAVGDD1863 pKa = 3.85 VLEE1866 pKa = 4.26 YY1867 pKa = 10.76 SITVEE1872 pKa = 4.23 NPSAAPASEE1881 pKa = 3.37 ITIYY1885 pKa = 11.04 DD1886 pKa = 3.28 RR1887 pKa = 11.84 TPPYY1891 pKa = 10.21 TEE1893 pKa = 4.24 LFAPIVSPSALGDD1906 pKa = 3.82 MICTLITPSGNSAGYY1921 pKa = 10.27 AGNLEE1926 pKa = 4.3 WDD1928 pKa = 3.97 CVGAFLPGSRR1938 pKa = 11.84 GNVAFQVQIAPP1949 pKa = 3.76
Molecular weight: 202.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.605
IPC_protein 3.656
Toseland 3.427
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.592
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.617
Nozaki 3.757
DTASelect 4.037
Thurlkill 3.465
EMBOSS 3.605
Sillero 3.783
Patrickios 1.1
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A3N4U8V9|A0A3N4U8V9_9RHOB Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pacificibacter maritimus OX=762213 GN=accA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.06 NLSAA44 pKa = 4.62
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2932
0
2932
919199
30
2595
313.5
34.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.319 ± 0.056
0.917 ± 0.015
6.401 ± 0.044
5.633 ± 0.046
3.947 ± 0.031
8.067 ± 0.049
2.018 ± 0.021
5.773 ± 0.03
4.11 ± 0.038
9.759 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.852 ± 0.021
3.032 ± 0.026
4.617 ± 0.031
3.516 ± 0.027
5.626 ± 0.04
5.827 ± 0.035
5.841 ± 0.04
7.19 ± 0.032
1.258 ± 0.02
2.298 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here