Salmonella phage vB_SenS_SE1
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A481W586|A0A481W586_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_SE1 OX=2530161 PE=4 SV=1
MM1 pKa = 6.5 VARR4 pKa = 11.84 YY5 pKa = 9.06 RR6 pKa = 11.84 FNYY9 pKa = 7.46 VQEE12 pKa = 4.15 MFEE15 pKa = 4.17 TDD17 pKa = 3.69 AGEE20 pKa = 4.25 QYY22 pKa = 11.04 CEE24 pKa = 4.14 TEE26 pKa = 3.48 RR27 pKa = 11.84 DD28 pKa = 2.94 EE29 pKa = 4.13 WVRR32 pKa = 11.84 YY33 pKa = 8.68 EE34 pKa = 5.5 DD35 pKa = 3.76 YY36 pKa = 11.67 AEE38 pKa = 4.33 LKK40 pKa = 10.48 EE41 pKa = 4.67 LLDD44 pKa = 3.9 ALAYY48 pKa = 10.45 SVLNYY53 pKa = 9.63 PLSPDD58 pKa = 3.48 TEE60 pKa = 4.14 EE61 pKa = 3.98 LAKK64 pKa = 10.76 GVLGVV69 pKa = 3.67
Molecular weight: 8.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.884
IPC2_protein 4.151
IPC_protein 4.012
Toseland 3.859
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.126
Wikipedia 3.834
Rodwell 3.859
Grimsley 3.77
Solomon 3.948
Lehninger 3.897
Nozaki 4.088
DTASelect 4.19
Thurlkill 3.884
EMBOSS 3.859
Sillero 4.126
Patrickios 0.846
IPC_peptide 3.961
IPC2_peptide 4.113
IPC2.peptide.svr19 4.016
Protein with the highest isoelectric point:
>tr|A0A481W6Z8|A0A481W6Z8_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_SE1 OX=2530161 PE=4 SV=1
MM1 pKa = 7.52 SLATDD6 pKa = 3.38 ILKK9 pKa = 10.37 RR10 pKa = 11.84 ANSDD14 pKa = 3.34 LSPPSKK20 pKa = 10.44 SIRR23 pKa = 11.84 ADD25 pKa = 3.31 YY26 pKa = 9.1 MGPAIRR32 pKa = 11.84 KK33 pKa = 6.74 KK34 pKa = 10.59 AKK36 pKa = 9.47 RR37 pKa = 11.84 AAPKK41 pKa = 10.45 GPRR44 pKa = 11.84 LNRR47 pKa = 11.84 KK48 pKa = 4.78 TVNYY52 pKa = 8.44 VAPKK56 pKa = 8.35 HH57 pKa = 4.95 EE58 pKa = 4.5 HH59 pKa = 4.99 VAGVAFNSRR68 pKa = 11.84 NNTWDD73 pKa = 3.12 AYY75 pKa = 10.49 FYY77 pKa = 11.23 NGVKK81 pKa = 9.82 TIRR84 pKa = 11.84 IGMFHH89 pKa = 5.71 TQARR93 pKa = 11.84 ALIARR98 pKa = 11.84 RR99 pKa = 11.84 IYY101 pKa = 9.73 MYY103 pKa = 9.49 WRR105 pKa = 11.84 KK106 pKa = 10.11 CGFDD110 pKa = 3.89 NIPNKK115 pKa = 9.56 PEE117 pKa = 3.47 RR118 pKa = 11.84 RR119 pKa = 11.84 LYY121 pKa = 10.15 TMRR124 pKa = 11.84 NYY126 pKa = 10.69 SDD128 pKa = 3.46 KK129 pKa = 11.39 SS130 pKa = 3.41
Molecular weight: 15.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 10.072
IPC_protein 10.833
Toseland 10.833
ProMoST 10.54
Dawson 10.935
Bjellqvist 10.657
Wikipedia 11.155
Rodwell 11.242
Grimsley 10.994
Solomon 11.052
Lehninger 11.008
Nozaki 10.804
DTASelect 10.657
Thurlkill 10.833
EMBOSS 11.228
Sillero 10.862
Patrickios 10.95
IPC_peptide 11.052
IPC2_peptide 9.56
IPC2.peptide.svr19 8.194
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
63
0
63
12358
38
852
196.2
21.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.293 ± 0.589
1.084 ± 0.121
6.441 ± 0.252
6.441 ± 0.369
3.423 ± 0.16
7.566 ± 0.339
1.626 ± 0.191
5.09 ± 0.203
5.697 ± 0.355
7.606 ± 0.282
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.492 ± 0.111
4.758 ± 0.253
3.941 ± 0.255
4.256 ± 0.334
5.268 ± 0.209
5.931 ± 0.288
6.247 ± 0.372
7.04 ± 0.332
1.497 ± 0.171
3.302 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here