Wallemia ichthyophaga
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4149 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V4M5V1|A0A4V4M5V1_WALIC Pentafunctional AROM polypeptide OS=Wallemia ichthyophaga OX=245174 GN=E3P86_01585 PE=3 SV=1
MM1 pKa = 7.3 VLLKK5 pKa = 9.84 TATTLLAATAAYY17 pKa = 9.34 AWNHH21 pKa = 5.09 TASDD25 pKa = 4.22 VIATGTMGTTNPPEE39 pKa = 3.92 ATMGTEE45 pKa = 4.46 LNQTSVARR53 pKa = 11.84 LVSINSIDD61 pKa = 3.76 DD62 pKa = 3.49 FCFFGPKK69 pKa = 8.57 EE70 pKa = 4.15 ANVTIGDD77 pKa = 3.93 TEE79 pKa = 4.47 GEE81 pKa = 4.17 QVAWCTKK88 pKa = 8.17 PRR90 pKa = 11.84 NNARR94 pKa = 11.84 VIPDD98 pKa = 3.27 GTITSTHH105 pKa = 5.23 FVKK108 pKa = 10.2 TPAYY112 pKa = 8.72 VQVSGFGDD120 pKa = 3.53 LTKK123 pKa = 10.41 IGVKK127 pKa = 10.51 DD128 pKa = 3.4 GDD130 pKa = 3.85 YY131 pKa = 11.16 GGEE134 pKa = 4.16 LDD136 pKa = 3.7 PHH138 pKa = 6.59 GATGDD143 pKa = 3.82 GNPVGGNVTSDD154 pKa = 2.95 IVNGTSTSYY163 pKa = 11.27 AEE165 pKa = 3.67 WMEE168 pKa = 4.58 FISYY172 pKa = 9.35 EE173 pKa = 3.97 QFCIRR178 pKa = 11.84 ICTASNDD185 pKa = 3.28 TWGAPVMCEE194 pKa = 3.73 HH195 pKa = 7.14 KK196 pKa = 10.3 LDD198 pKa = 3.69 EE199 pKa = 4.55 MGCGFVMPDD208 pKa = 3.64 AGDD211 pKa = 3.39 SDD213 pKa = 4.49 VFEE216 pKa = 4.6 TCDD219 pKa = 3.95 ADD221 pKa = 3.74 VAYY224 pKa = 9.41 PPGVYY229 pKa = 9.95 PIDD232 pKa = 3.88 GSYY235 pKa = 9.18 STFAQRR241 pKa = 11.84 YY242 pKa = 4.82 TGSYY246 pKa = 10.64 SSGTTGAMVGYY257 pKa = 7.88 TVGVTDD263 pKa = 4.48 TPTAAQMTPSSSNCVTQSSLAGNNVDD289 pKa = 3.67 VSATGVIEE297 pKa = 4.95 DD298 pKa = 4.72 NRR300 pKa = 11.84 DD301 pKa = 3.36 DD302 pKa = 5.91 DD303 pKa = 4.89 SDD305 pKa = 4.76 SNSDD309 pKa = 3.64 PDD311 pKa = 4.24 SNEE314 pKa = 3.81 SDD316 pKa = 3.42 KK317 pKa = 11.83 SNPDD321 pKa = 3.15 GNGGGSEE328 pKa = 4.13 PTDD331 pKa = 3.29 NGQTSANAGDD341 pKa = 4.41 GDD343 pKa = 4.17 SSATTFTGFATVSAIALAVLAIALL367 pKa = 4.06
Molecular weight: 37.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.77
IPC_protein 3.795
Toseland 3.567
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.478
Solomon 3.795
Lehninger 3.745
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.923
Patrickios 0.68
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.807
Protein with the highest isoelectric point:
>tr|A0A4T0J315|A0A4T0J315_WALIC Uncharacterized protein OS=Wallemia ichthyophaga OX=245174 GN=E3P86_02399 PE=4 SV=1
MM1 pKa = 7.43 PRR3 pKa = 11.84 KK4 pKa = 9.67 IPLFPTFTTLLNRR17 pKa = 11.84 RR18 pKa = 11.84 PTLPAISAIAANGLRR33 pKa = 11.84 FGSRR37 pKa = 11.84 GTDD40 pKa = 3.46 YY41 pKa = 11.15 QPSTRR46 pKa = 11.84 KK47 pKa = 9.59 RR48 pKa = 11.84 KK49 pKa = 8.51 RR50 pKa = 11.84 TFGFLARR57 pKa = 11.84 IRR59 pKa = 11.84 SRR61 pKa = 11.84 TGRR64 pKa = 11.84 KK65 pKa = 8.96 IISRR69 pKa = 11.84 RR70 pKa = 11.84 LKK72 pKa = 10.29 KK73 pKa = 10.25 GKK75 pKa = 10.45 KK76 pKa = 9.46 NMSHH80 pKa = 6.78
Molecular weight: 9.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.394
IPC2_protein 10.979
IPC_protein 12.413
Toseland 12.574
ProMoST 13.071
Dawson 12.574
Bjellqvist 12.574
Wikipedia 13.056
Rodwell 12.296
Grimsley 12.618
Solomon 13.071
Lehninger 12.969
Nozaki 12.574
DTASelect 12.574
Thurlkill 12.574
EMBOSS 13.071
Sillero 12.574
Patrickios 12.018
IPC_peptide 13.071
IPC2_peptide 12.062
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4149
0
4149
2250108
53
4950
542.3
60.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.524 ± 0.033
1.058 ± 0.011
6.198 ± 0.026
6.125 ± 0.035
3.637 ± 0.022
5.871 ± 0.033
2.743 ± 0.019
5.39 ± 0.025
5.815 ± 0.033
9.224 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.057 ± 0.011
4.778 ± 0.024
5.149 ± 0.029
4.463 ± 0.03
5.389 ± 0.023
8.932 ± 0.038
5.758 ± 0.018
5.931 ± 0.024
1.102 ± 0.009
2.854 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here