Cetacean poxvirus 1
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B9R4L0|A0A6B9R4L0_9POXV DNA topoisomerase I OS=Cetacean poxvirus 1 OX=319748 PE=3 SV=1
MM1 pKa = 7.3 GVNHH5 pKa = 6.66 EE6 pKa = 4.85 LDD8 pKa = 3.78 VIINNVTFKK17 pKa = 11.14 NVGLIKK23 pKa = 10.38 GDD25 pKa = 3.45 SYY27 pKa = 11.75 GCHH30 pKa = 6.8 IDD32 pKa = 5.78 LISTDD37 pKa = 2.72 IDD39 pKa = 4.16 YY40 pKa = 10.69 IVCLTVILRR49 pKa = 11.84 PDD51 pKa = 4.33 WINVNKK57 pKa = 10.01 VKK59 pKa = 10.62 HH60 pKa = 5.92 IRR62 pKa = 11.84 LKK64 pKa = 9.29 VNSYY68 pKa = 9.06 EE69 pKa = 4.79 LEE71 pKa = 4.23 LNVKK75 pKa = 10.2 KK76 pKa = 9.21 EE77 pKa = 4.33 TNEE80 pKa = 3.68 EE81 pKa = 4.48 VIWEE85 pKa = 4.14 TDD87 pKa = 3.77 FVGHH91 pKa = 6.57 NVTTLKK97 pKa = 10.85 LYY99 pKa = 10.22 TDD101 pKa = 4.22 EE102 pKa = 4.91 NDD104 pKa = 3.11 EE105 pKa = 4.17 SKK107 pKa = 11.44 YY108 pKa = 10.63 PVLQYY113 pKa = 10.14 NTFRR117 pKa = 11.84 RR118 pKa = 11.84 HH119 pKa = 4.63 AAVMYY124 pKa = 9.83 EE125 pKa = 4.16 GYY127 pKa = 9.26 TYY129 pKa = 9.89 GICIKK134 pKa = 10.67 YY135 pKa = 10.25 DD136 pKa = 3.44 DD137 pKa = 5.2 NYY139 pKa = 11.17 DD140 pKa = 4.16 DD141 pKa = 6.03 NYY143 pKa = 11.65 DD144 pKa = 5.34 DD145 pKa = 6.73 DD146 pKa = 7.31 DD147 pKa = 7.56 DD148 pKa = 7.6 DD149 pKa = 7.66 DD150 pKa = 7.53 DD151 pKa = 7.65 DD152 pKa = 7.65 DD153 pKa = 7.65 DD154 pKa = 7.65 DD155 pKa = 7.65 DD156 pKa = 7.58 DD157 pKa = 7.56 DD158 pKa = 7.49 DD159 pKa = 7.11 DD160 pKa = 7.31 DD161 pKa = 5.13 DD162 pKa = 6.35 VIVIDD167 pKa = 4.08
Molecular weight: 19.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.846
IPC_protein 3.884
Toseland 3.643
ProMoST 4.037
Dawson 3.897
Bjellqvist 4.062
Wikipedia 3.846
Rodwell 3.706
Grimsley 3.554
Solomon 3.884
Lehninger 3.846
Nozaki 3.999
DTASelect 4.304
Thurlkill 3.706
EMBOSS 3.859
Sillero 4.012
Patrickios 1.926
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.901
Protein with the highest isoelectric point:
>tr|A0A6B9R5X2|A0A6B9R5X2_9POXV EEv membrane phosphoglycoprotein OS=Cetacean poxvirus 1 OX=319748 PE=4 SV=1
MM1 pKa = 7.07 YY2 pKa = 9.06 WYY4 pKa = 10.78 SNYY7 pKa = 10.54 NIVLTPPRR15 pKa = 11.84 RR16 pKa = 11.84 CTKK19 pKa = 10.36 CAANLTAMLIEE30 pKa = 4.82 DD31 pKa = 4.08 KK32 pKa = 11.45 QNIKK36 pKa = 10.57 YY37 pKa = 10.59 LLYY40 pKa = 10.6 SQPNKK45 pKa = 10.59 LKK47 pKa = 10.94 LILDD51 pKa = 4.51 FLTVSRR57 pKa = 11.84 NKK59 pKa = 10.5 SFNLKK64 pKa = 10.0 ILDD67 pKa = 3.72 KK68 pKa = 10.86 EE69 pKa = 4.39 IIRR72 pKa = 11.84 VLTT75 pKa = 3.69
Molecular weight: 8.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.167
IPC2_protein 9.194
IPC_protein 9.136
Toseland 9.721
ProMoST 9.487
Dawson 9.984
Bjellqvist 9.692
Wikipedia 10.16
Rodwell 10.335
Grimsley 10.058
Solomon 10.028
Lehninger 9.984
Nozaki 9.794
DTASelect 9.663
Thurlkill 9.823
EMBOSS 10.145
Sillero 9.911
Patrickios 7.527
IPC_peptide 10.014
IPC2_peptide 8.448
IPC2.peptide.svr19 8.091
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
115
0
115
36852
54
1288
320.5
37.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.909 ± 0.126
2.42 ± 0.133
5.894 ± 0.142
5.397 ± 0.156
4.827 ± 0.136
3.275 ± 0.141
2.019 ± 0.084
10.827 ± 0.189
8.171 ± 0.145
9.37 ± 0.191
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.41 ± 0.087
8.13 ± 0.176
2.781 ± 0.112
2.385 ± 0.088
3.468 ± 0.12
7.956 ± 0.147
5.785 ± 0.143
5.886 ± 0.112
0.559 ± 0.037
5.53 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here