Joostella marina DSM 19592

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Joostella; Joostella marina

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3783 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3CB81|I3CB81_9FLAO Uncharacterized protein OS=Joostella marina DSM 19592 OX=926559 GN=JoomaDRAFT_3950 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.09VNKK5 pKa = 10.02IIGLWGSVLILLFSACQPIEE25 pKa = 4.0DD26 pKa = 4.41RR27 pKa = 11.84EE28 pKa = 4.14EE29 pKa = 4.43LEE31 pKa = 4.18NTTDD35 pKa = 3.59ADD37 pKa = 4.07GVKK40 pKa = 10.21LVATQATAGGNLVEE54 pKa = 5.84LSMEE58 pKa = 4.23TLGVTGYY65 pKa = 10.46WDD67 pKa = 3.78FNLGKK72 pKa = 10.45GLTDD76 pKa = 4.63RR77 pKa = 11.84IEE79 pKa = 3.91IVYY82 pKa = 7.46PIPGATTFTYY92 pKa = 10.66VGTLGGEE99 pKa = 4.51FFSKK103 pKa = 9.95TIDD106 pKa = 3.65VQIDD110 pKa = 3.84TLDD113 pKa = 3.51HH114 pKa = 6.95ALNQDD119 pKa = 2.87WYY121 pKa = 10.14DD122 pKa = 3.72LVSEE126 pKa = 4.3EE127 pKa = 4.28TSVGKK132 pKa = 8.33TWVFAGGPGADD143 pKa = 3.67GGKK146 pKa = 7.22WWYY149 pKa = 9.04MSPPDD154 pKa = 5.72DD155 pKa = 3.96PASWEE160 pKa = 4.28TAWWNAAGDD169 pKa = 4.11CCPPGDD175 pKa = 4.49AAGKK179 pKa = 8.18MHH181 pKa = 7.38FDD183 pKa = 3.81LDD185 pKa = 3.77GAGNYY190 pKa = 7.98TYY192 pKa = 11.28YY193 pKa = 11.04SDD195 pKa = 3.57TDD197 pKa = 3.72VEE199 pKa = 4.66GEE201 pKa = 4.15TGSFVLDD208 pKa = 3.49VANQTLQVNGSNILGAEE225 pKa = 4.02QGNPAGLYY233 pKa = 9.2TIISLTEE240 pKa = 4.21DD241 pKa = 3.4EE242 pKa = 5.55LILYY246 pKa = 7.55LANNAGGTGWTWVFKK261 pKa = 11.03PEE263 pKa = 3.77

Molecular weight: 28.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3C581|I3C581_9FLAO Bacterial cell division membrane protein OS=Joostella marina DSM 19592 OX=926559 GN=JoomaDRAFT_1767 PE=4 SV=1
MM1 pKa = 7.26LRR3 pKa = 11.84WTIIFLIVAIVAAVFGFGGIAGAAAGIAKK32 pKa = 9.99ILFFVFLVLFVISLISRR49 pKa = 11.84MTRR52 pKa = 11.84RR53 pKa = 3.46

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3783

0

3783

1268527

29

4610

335.3

37.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.37 ± 0.039

0.699 ± 0.014

5.478 ± 0.034

6.98 ± 0.041

5.057 ± 0.029

6.353 ± 0.039

1.713 ± 0.017

8.034 ± 0.041

8.026 ± 0.053

9.139 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.182 ± 0.018

6.283 ± 0.038

3.347 ± 0.022

3.298 ± 0.024

3.359 ± 0.027

6.45 ± 0.031

5.758 ± 0.041

6.148 ± 0.028

1.084 ± 0.015

4.24 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski