Aspergillus mulundensis
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11569 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3D8R417|A0A3D8R417_9EURO Cupin_2 domain-containing protein OS=Aspergillus mulundensis OX=1810919 GN=DSM5745_08562 PE=4 SV=1
MM1 pKa = 7.31 YY2 pKa = 9.98 YY3 pKa = 11.09 ALGSLALVAFATEE16 pKa = 4.54 VLATPHH22 pKa = 6.18 PMITASPTLAKK33 pKa = 9.93 RR34 pKa = 11.84 DD35 pKa = 3.41 SCTFSGSDD43 pKa = 3.46 GAASASKK50 pKa = 10.4 SQADD54 pKa = 4.03 CATITLSDD62 pKa = 3.27 ITVPSGTTLDD72 pKa = 4.41 LSDD75 pKa = 5.7 LEE77 pKa = 6.28 DD78 pKa = 3.8 DD79 pKa = 3.79 TTVVFEE85 pKa = 4.8 GTTSWEE91 pKa = 4.01 YY92 pKa = 11.19 EE93 pKa = 4.1 EE94 pKa = 4.73 WDD96 pKa = 4.42 GPLLQIKK103 pKa = 8.39 GTGITIKK110 pKa = 10.69 GADD113 pKa = 3.79 GAKK116 pKa = 10.27 LNPDD120 pKa = 3.7 GSRR123 pKa = 11.84 WWDD126 pKa = 3.4 GEE128 pKa = 3.98 GSNGGVTKK136 pKa = 10.54 PKK138 pKa = 10.33 FFYY141 pKa = 10.56 AHH143 pKa = 6.09 SLKK146 pKa = 10.95 DD147 pKa = 3.43 STIEE151 pKa = 3.61 NLYY154 pKa = 10.36 IEE156 pKa = 4.64 NTPVQAVSINSCDD169 pKa = 3.44 GLTITDD175 pKa = 3.54 MTIDD179 pKa = 3.6 NSAGDD184 pKa = 3.91 DD185 pKa = 3.66 AGGHH189 pKa = 5.07 NTDD192 pKa = 4.03 GFDD195 pKa = 3.12 IGEE198 pKa = 4.42 SNNVVITGAKK208 pKa = 9.79 VYY210 pKa = 10.84 NQDD213 pKa = 3.04 DD214 pKa = 4.2 CVAVNSGTEE223 pKa = 3.75 ITFSGGTCSGGHH235 pKa = 5.98 GLSIGSVGGRR245 pKa = 11.84 DD246 pKa = 4.03 DD247 pKa = 3.81 NTVDD251 pKa = 3.1 TVTFKK256 pKa = 11.29 DD257 pKa = 3.66 STVSNSVNGIRR268 pKa = 11.84 IKK270 pKa = 10.87 AKK272 pKa = 9.98 SDD274 pKa = 3.02 EE275 pKa = 4.2 TGEE278 pKa = 4.34 IKK280 pKa = 10.66 GVTYY284 pKa = 10.78 SGITLDD290 pKa = 4.48 SISKK294 pKa = 9.82 YY295 pKa = 10.83 GILIEE300 pKa = 3.98 QNYY303 pKa = 10.68 DD304 pKa = 3.16 GGDD307 pKa = 3.43 LHH309 pKa = 7.46 GDD311 pKa = 3.57 PTSGIPITDD320 pKa = 3.6 LTIEE324 pKa = 5.08 DD325 pKa = 3.77 ISGSGAVDD333 pKa = 3.11 SDD335 pKa = 3.93 GYY337 pKa = 11.22 NIVVVCGDD345 pKa = 4.74 DD346 pKa = 4.43 GCSDD350 pKa = 3.61 WTWSGVEE357 pKa = 3.83 VSGGEE362 pKa = 4.52 DD363 pKa = 3.46 YY364 pKa = 11.38 SDD366 pKa = 4.34 CEE368 pKa = 4.3 NVPSVASCSTT378 pKa = 3.26
Molecular weight: 39.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.808
IPC_protein 3.834
Toseland 3.605
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.516
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.215
Thurlkill 3.656
EMBOSS 3.795
Sillero 3.961
Patrickios 1.341
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A3D8QJG5|A0A3D8QJG5_9EURO Ankyrin repeat protein OS=Aspergillus mulundensis OX=1810919 GN=DSM5745_10492 PE=4 SV=1
MM1 pKa = 7.68 SIGAYY6 pKa = 8.58 EE7 pKa = 4.02 VVMCGEE13 pKa = 4.77 DD14 pKa = 3.6 EE15 pKa = 4.07 QSEE18 pKa = 4.28 ALISPSHH25 pKa = 6.61 RR26 pKa = 11.84 NGFKK30 pKa = 10.05 PAPMCKK36 pKa = 9.44 DD37 pKa = 3.72 DD38 pKa = 3.61 NHH40 pKa = 6.71 GATEE44 pKa = 4.02 YY45 pKa = 10.03 WISEE49 pKa = 4.27 SEE51 pKa = 4.27 RR52 pKa = 11.84 PHH54 pKa = 5.8 PRR56 pKa = 11.84 AVRR59 pKa = 11.84 SSTNEE64 pKa = 3.54 PAHH67 pKa = 6.51 PRR69 pKa = 11.84 TPSPPKK75 pKa = 10.36 HH76 pKa = 6.22 RR77 pKa = 11.84 VTTCSQTVRR86 pKa = 11.84 RR87 pKa = 11.84 CQTHH91 pKa = 6.27 IGIHH95 pKa = 5.33 HH96 pKa = 7.22 RR97 pKa = 11.84 NNKK100 pKa = 8.28 GAPAHH105 pKa = 5.76 QNPDD109 pKa = 3.03 VAPRR113 pKa = 11.84 KK114 pKa = 9.31 ARR116 pKa = 11.84 HH117 pKa = 5.93 APFPRR122 pKa = 11.84 PPHH125 pKa = 5.63 PLRR128 pKa = 11.84 RR129 pKa = 11.84 RR130 pKa = 11.84 LQCIAHH136 pKa = 6.51 AFGYY140 pKa = 8.24 TPHH143 pKa = 7.21 EE144 pKa = 4.57 IIGIVFDD151 pKa = 4.83 RR152 pKa = 11.84 PVLAPNMAPANEE164 pKa = 4.04 ALKK167 pKa = 10.2 EE168 pKa = 3.88 ARR170 pKa = 11.84 IYY172 pKa = 10.36 NHH174 pKa = 6.35 NEE176 pKa = 3.62 SPRR179 pKa = 11.84 PARR182 pKa = 11.84 AGVYY186 pKa = 10.48 LLLLHH191 pKa = 7.13 RR192 pKa = 11.84 APATGGEE199 pKa = 4.27 TTIPSSLEE207 pKa = 3.41 LFRR210 pKa = 11.84 RR211 pKa = 11.84 ARR213 pKa = 11.84 AEE215 pKa = 3.93 MPTSLTCWRR224 pKa = 11.84 RR225 pKa = 11.84 GHH227 pKa = 6.6 HH228 pKa = 6.22 EE229 pKa = 4.19 PGHH232 pKa = 5.45 VYY234 pKa = 10.27 GRR236 pKa = 11.84 AAVRR240 pKa = 11.84 GRR242 pKa = 11.84 VDD244 pKa = 4.02 DD245 pKa = 3.72 EE246 pKa = 4.3 TGVRR250 pKa = 11.84 KK251 pKa = 9.77 EE252 pKa = 4.55 FADD255 pKa = 3.74 TDD257 pKa = 3.77 DD258 pKa = 6.06 DD259 pKa = 3.7 EE260 pKa = 4.69 TKK262 pKa = 10.25 RR263 pKa = 11.84 RR264 pKa = 11.84 KK265 pKa = 9.91 VEE267 pKa = 3.82 AQIARR272 pKa = 11.84 YY273 pKa = 9.53 GRR275 pKa = 11.84 GRR277 pKa = 11.84 HH278 pKa = 4.95 TTSEE282 pKa = 3.82 WTDD285 pKa = 3.35 DD286 pKa = 3.55 DD287 pKa = 4.64 RR288 pKa = 11.84 EE289 pKa = 4.02 RR290 pKa = 11.84 LQLVLTHH297 pKa = 6.6 HH298 pKa = 7.23 LPRR301 pKa = 11.84 VPSARR306 pKa = 11.84 NPEE309 pKa = 4.05 RR310 pKa = 11.84 TFPLPTPFRR319 pKa = 11.84 ASGVPQEE326 pKa = 4.53 RR327 pKa = 11.84 DD328 pKa = 2.78 RR329 pKa = 11.84 SQEE332 pKa = 3.89 GKK334 pKa = 9.83 SRR336 pKa = 11.84 RR337 pKa = 11.84 APGQEE342 pKa = 3.32 RR343 pKa = 11.84 RR344 pKa = 11.84 ASGVWRR350 pKa = 11.84 RR351 pKa = 11.84 HH352 pKa = 5.2 ADD354 pKa = 3.16 SGGGPGGAGAHH365 pKa = 6.71 HH366 pKa = 6.53 GRR368 pKa = 11.84 DD369 pKa = 3.52 PRR371 pKa = 11.84 ATSMAGGRR379 pKa = 11.84 RR380 pKa = 11.84 AGLRR384 pKa = 11.84 QCRR387 pKa = 11.84 CAAWAGAVGRR397 pKa = 11.84 GAGGSRR403 pKa = 11.84 CSGG406 pKa = 3.18
Molecular weight: 44.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.399
IPC_protein 10.365
Toseland 10.482
ProMoST 10.292
Dawson 10.599
Bjellqvist 10.379
Wikipedia 10.847
Rodwell 10.613
Grimsley 10.657
Solomon 10.745
Lehninger 10.701
Nozaki 10.526
DTASelect 10.365
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.555
Patrickios 10.262
IPC_peptide 10.745
IPC2_peptide 9.721
IPC2.peptide.svr19 8.585
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11569
0
11569
5851458
50
7188
505.8
55.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.791 ± 0.019
1.249 ± 0.008
5.743 ± 0.014
6.22 ± 0.02
3.677 ± 0.012
6.917 ± 0.021
2.426 ± 0.009
4.855 ± 0.014
4.411 ± 0.021
9.158 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.06 ± 0.009
3.584 ± 0.011
6.112 ± 0.022
3.918 ± 0.016
6.225 ± 0.021
8.171 ± 0.024
5.979 ± 0.016
6.199 ± 0.015
1.487 ± 0.008
2.818 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here