Sulfolobus filamentous virus 1
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346LUA3|A0A346LUA3_9VIRU Uncharacterized protein OS=Sulfolobus filamentous virus 1 OX=2304198 GN=SFV1gp64 PE=4 SV=1
MM1 pKa = 7.48 NKK3 pKa = 10.07 DD4 pKa = 2.96 DD5 pKa = 4.4 WLEE8 pKa = 4.18 KK9 pKa = 10.02 IYY11 pKa = 9.59 TAISYY16 pKa = 9.41 YY17 pKa = 10.19 IGDD20 pKa = 3.88 IASDD24 pKa = 3.81 DD25 pKa = 3.96 DD26 pKa = 4.46 LSEE29 pKa = 4.42 KK30 pKa = 10.68 EE31 pKa = 4.51 KK32 pKa = 10.97 EE33 pKa = 3.98 QLKK36 pKa = 10.9 NMLYY40 pKa = 10.67 DD41 pKa = 3.48 FLGAIEE47 pKa = 5.35 NFDD50 pKa = 3.57 EE51 pKa = 4.51 YY52 pKa = 10.96 EE53 pKa = 4.15 QEE55 pKa = 4.09 EE56 pKa = 4.51 EE57 pKa = 4.07
Molecular weight: 6.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.034
IPC2_protein 3.821
IPC_protein 3.732
Toseland 3.554
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.935
Wikipedia 3.63
Rodwell 3.579
Grimsley 3.465
Solomon 3.694
Lehninger 3.643
Nozaki 3.846
DTASelect 3.999
Thurlkill 3.605
EMBOSS 3.643
Sillero 3.859
Patrickios 2.943
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A346LU41|A0A346LU41_9VIRU Putative superfamily 2 helicase OS=Sulfolobus filamentous virus 1 OX=2304198 GN=SFV1gp02 PE=4 SV=1
MM1 pKa = 7.97 EE2 pKa = 5.47 DD3 pKa = 2.67 TGGWYY8 pKa = 10.23 RR9 pKa = 11.84 KK10 pKa = 9.79 NKK12 pKa = 9.38 GGNSRR17 pKa = 11.84 EE18 pKa = 4.18 KK19 pKa = 10.13 NVGWRR24 pKa = 11.84 KK25 pKa = 8.39 MVAKK29 pKa = 7.96 NTCVKK34 pKa = 9.51 VKK36 pKa = 10.41 KK37 pKa = 9.75 MYY39 pKa = 10.16 IGLGFALLGRR49 pKa = 11.84 MINRR53 pKa = 11.84 KK54 pKa = 8.8
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.274
IPC2_protein 9.897
IPC_protein 10.277
Toseland 10.921
ProMoST 10.511
Dawson 10.994
Bjellqvist 10.613
Wikipedia 11.125
Rodwell 11.506
Grimsley 11.008
Solomon 11.067
Lehninger 11.038
Nozaki 10.891
DTASelect 10.599
Thurlkill 10.891
EMBOSS 11.301
Sillero 10.906
Patrickios 11.257
IPC_peptide 11.067
IPC2_peptide 9.311
IPC2.peptide.svr19 8.563
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
65
0
65
11860
35
617
182.5
20.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.486 ± 0.252
1.206 ± 0.119
4.907 ± 0.331
5.818 ± 0.442
4.899 ± 0.261
4.874 ± 0.321
1.366 ± 0.139
9.334 ± 0.315
7.31 ± 0.496
9.486 ± 0.212
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.403 ± 0.155
6.29 ± 0.275
3.415 ± 0.285
3.558 ± 0.252
3.845 ± 0.304
5.97 ± 0.343
6.83 ± 0.436
7.411 ± 0.26
0.776 ± 0.081
5.818 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here