Ralstonia phage Dimitile

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Cimandefvirus; unclassified Cimandefvirus

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5B995|A0A7G5B995_9CAUD Peptidase S74 domain-containing protein OS=Ralstonia phage Dimitile OX=2759723 GN=D1_00042 PE=4 SV=1
MM1 pKa = 7.53LGIVQKK7 pKa = 9.44TATEE11 pKa = 3.89QLDD14 pKa = 3.56YY15 pKa = 11.26DD16 pKa = 3.64IDD18 pKa = 3.85FARR21 pKa = 11.84WMPDD25 pKa = 2.55GDD27 pKa = 4.34LLQSAGVVITPDD39 pKa = 4.17DD40 pKa = 3.68GTLASPAYY48 pKa = 9.25EE49 pKa = 3.65IDD51 pKa = 3.57GTVVKK56 pKa = 10.55VWLAGGTAGASYY68 pKa = 11.0NVDD71 pKa = 3.27VTVATAAGRR80 pKa = 11.84IKK82 pKa = 9.03EE83 pKa = 4.4TCFKK87 pKa = 10.58IRR89 pKa = 11.84VRR91 pKa = 11.84SCC93 pKa = 2.82

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5B958|A0A7G5B958_9CAUD Uncharacterized protein OS=Ralstonia phage Dimitile OX=2759723 GN=D1_00005 PE=4 SV=1
MM1 pKa = 7.58SLTQEE6 pKa = 4.11FLKK9 pKa = 11.02SVLTGIFVWVGKK21 pKa = 9.76PNGRR25 pKa = 11.84VEE27 pKa = 4.2AQAIAGSLHH36 pKa = 5.71SSGYY40 pKa = 8.77IHH42 pKa = 7.26IGIQRR47 pKa = 11.84RR48 pKa = 11.84VYY50 pKa = 9.87KK51 pKa = 10.39AHH53 pKa = 6.85RR54 pKa = 11.84LAWLYY59 pKa = 7.37MTGEE63 pKa = 4.78FPSSMLDD70 pKa = 3.18HH71 pKa = 7.01KK72 pKa = 11.47NGDD75 pKa = 3.35RR76 pKa = 11.84SDD78 pKa = 3.9NRR80 pKa = 11.84WANIRR85 pKa = 11.84QADD88 pKa = 3.87STKK91 pKa = 10.24NCMNRR96 pKa = 11.84ACRR99 pKa = 11.84SDD101 pKa = 2.98SRR103 pKa = 11.84TGMKK107 pKa = 9.76GVRR110 pKa = 11.84YY111 pKa = 9.76DD112 pKa = 3.38GRR114 pKa = 11.84DD115 pKa = 3.15GRR117 pKa = 11.84FQAVIMISGKK127 pKa = 10.08SFSLGRR133 pKa = 11.84FASAEE138 pKa = 4.11DD139 pKa = 3.18AHH141 pKa = 6.22AAYY144 pKa = 8.99MKK146 pKa = 10.47AANDD150 pKa = 3.32NFGEE154 pKa = 4.19FRR156 pKa = 11.84VQPFRR161 pKa = 11.84ARR163 pKa = 11.84SGKK166 pKa = 8.4KK167 pKa = 5.3TTPAVV172 pKa = 3.3

Molecular weight: 19.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

17721

31

4545

276.9

30.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.934 ± 0.604

0.762 ± 0.204

6.021 ± 0.21

6.292 ± 0.46

3.149 ± 0.169

8.724 ± 0.38

1.794 ± 0.126

4.385 ± 0.254

4.904 ± 0.316

6.93 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.118

3.73 ± 0.239

4.706 ± 0.207

4.323 ± 0.254

7.031 ± 0.311

5.654 ± 0.269

5.479 ± 0.319

6.495 ± 0.218

1.315 ± 0.148

2.68 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski