Acidianus filamentous virus 9

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Lipothrixviridae; Betalipothrixvirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2CRM5|B2CRM5_9VIRU Uncharacterized protein OS=Acidianus filamentous virus 9 OX=512792 PE=4 SV=1
MM1 pKa = 7.37SLCEE5 pKa = 3.9SWVQNVLPTGTVYY18 pKa = 10.83KK19 pKa = 10.44IATAYY24 pKa = 10.54FMYY27 pKa = 10.45NSTVVGQGSVTVSISYY43 pKa = 10.74DD44 pKa = 3.01KK45 pKa = 11.11ASAMFILTLTITDD58 pKa = 3.81NSTNTYY64 pKa = 8.39TYY66 pKa = 11.15NAILVKK72 pKa = 10.6DD73 pKa = 3.84SNGNNVVLFQYY84 pKa = 10.17SQAYY88 pKa = 9.34NKK90 pKa = 8.98GTGSLTVTEE99 pKa = 4.56YY100 pKa = 10.49IYY102 pKa = 10.81INDD105 pKa = 3.73VPPITEE111 pKa = 3.65ITSQFTASGDD121 pKa = 3.1WSEE124 pKa = 5.01IIANALVNGGAVYY137 pKa = 9.56MPNSVFVFDD146 pKa = 6.07DD147 pKa = 3.71NYY149 pKa = 11.13QAITQYY155 pKa = 11.29NFTPTTSIQGTSYY168 pKa = 10.76IEE170 pKa = 3.88QEE172 pKa = 4.42QISCTPCSSISGLYY186 pKa = 9.5VVLAYY191 pKa = 10.61YY192 pKa = 10.3DD193 pKa = 3.58QTSGTFIWVNYY204 pKa = 9.4QYY206 pKa = 10.47IASSFSVMNIIWSLSINPNCC226 pKa = 5.14

Molecular weight: 24.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2CRJ8|B2CRJ8_9VIRU Putative helicase OS=Acidianus filamentous virus 9 OX=512792 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.25KK3 pKa = 9.39ICLYY7 pKa = 10.25KK8 pKa = 10.84EE9 pKa = 4.01NVFTLSKK16 pKa = 10.51KK17 pKa = 9.09RR18 pKa = 11.84TIKK21 pKa = 10.7SIIHH25 pKa = 5.91YY26 pKa = 8.89IIKK29 pKa = 10.63GIILSSLSRR38 pKa = 11.84EE39 pKa = 3.48KK40 pKa = 10.87DD41 pKa = 3.1LYY43 pKa = 11.42KK44 pKa = 9.81MQKK47 pKa = 9.46HH48 pKa = 5.96KK49 pKa = 11.28SRR51 pKa = 11.84TT52 pKa = 3.58

Molecular weight: 6.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

12402

49

1170

169.9

19.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.443 ± 0.296

1.524 ± 0.229

4.62 ± 0.226

7.346 ± 0.388

4.588 ± 0.272

5.177 ± 0.216

1.371 ± 0.138

9.498 ± 0.462

7.837 ± 0.75

8.732 ± 0.479

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.185

5.362 ± 0.216

3.822 ± 0.334

3.217 ± 0.269

3.435 ± 0.352

6.475 ± 0.657

5.733 ± 0.498

7.039 ± 0.427

0.968 ± 0.104

6.322 ± 0.32
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski