Penicillium nalgiovense
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11728 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V6Y7V5|A0A1V6Y7V5_PENNA Uncharacterized protein OS=Penicillium nalgiovense OX=60175 GN=PENNAL_c0032G03885 PE=4 SV=1
MM1 pKa = 7.72 RR2 pKa = 11.84 TQQLLPLYY10 pKa = 9.5 GLLLASVAIASKK22 pKa = 10.51 LDD24 pKa = 3.57 HH25 pKa = 7.65 DD26 pKa = 5.06 DD27 pKa = 3.5 VPNRR31 pKa = 11.84 CWPACGPVVGIANSCDD47 pKa = 3.12 ARR49 pKa = 11.84 HH50 pKa = 5.93 EE51 pKa = 4.21 RR52 pKa = 11.84 DD53 pKa = 3.38 SAEE56 pKa = 3.95 IRR58 pKa = 11.84 CICDD62 pKa = 2.59 WDD64 pKa = 3.79 AAKK67 pKa = 8.95 TQIPLCAACITQYY80 pKa = 9.22 QTDD83 pKa = 3.72 RR84 pKa = 11.84 RR85 pKa = 11.84 NHH87 pKa = 6.42 KK88 pKa = 9.54 IAHH91 pKa = 6.95 HH92 pKa = 6.63 DD93 pKa = 3.77 HH94 pKa = 7.2 DD95 pKa = 5.99 HH96 pKa = 7.99 DD97 pKa = 7.1 DD98 pKa = 4.81 DD99 pKa = 6.82 DD100 pKa = 7.46 DD101 pKa = 6.34 YY102 pKa = 12.12 DD103 pKa = 6.5 DD104 pKa = 6.87 DD105 pKa = 7.28 DD106 pKa = 7.11 DD107 pKa = 7.43 DD108 pKa = 5.27 YY109 pKa = 12.12 DD110 pKa = 4.11 NDD112 pKa = 4.51 NDD114 pKa = 5.48 IDD116 pKa = 5.15 DD117 pKa = 5.56 DD118 pKa = 4.89 DD119 pKa = 6.11 NEE121 pKa = 4.39 ALDD124 pKa = 4.22 LVRR127 pKa = 11.84 SCTLTTTTYY136 pKa = 10.98 NSAAATTASTSTSTAGANTATTTDD160 pKa = 3.19 SSNAATGTDD169 pKa = 3.38 SAGTGGTNSQDD180 pKa = 3.46 STDD183 pKa = 3.73 SAEE186 pKa = 4.48 SAGSASSPTPTPDD199 pKa = 2.9 AAAGILAPGAVSMAGVMGLVALAWLL224 pKa = 4.2
Molecular weight: 23.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.682
IPC2_protein 3.821
IPC_protein 3.872
Toseland 3.63
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.884
Rodwell 3.694
Grimsley 3.528
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.355
Thurlkill 3.706
EMBOSS 3.884
Sillero 4.012
Patrickios 1.265
IPC_peptide 3.884
IPC2_peptide 3.973
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|A0A1V6YNW7|A0A1V6YNW7_PENNA Uncharacterized protein OS=Penicillium nalgiovense OX=60175 GN=PENNAL_c0015G01031 PE=4 SV=1
MM1 pKa = 7.87 PSHH4 pKa = 5.85 KK5 pKa = 9.4 TFRR8 pKa = 11.84 TKK10 pKa = 10.46 QKK12 pKa = 9.65 LAKK15 pKa = 9.74 AQRR18 pKa = 11.84 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TNNTIRR36 pKa = 11.84 YY37 pKa = 5.73 NAKK40 pKa = 8.78 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.52 WRR45 pKa = 11.84 KK46 pKa = 8.43 STLKK50 pKa = 10.46 VV51 pKa = 3.09
Molecular weight: 6.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11728
0
11728
5354899
8
7269
456.6
50.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.372 ± 0.019
1.26 ± 0.009
5.693 ± 0.016
6.145 ± 0.021
3.756 ± 0.014
6.71 ± 0.019
2.463 ± 0.011
5.021 ± 0.017
4.644 ± 0.017
8.929 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.257 ± 0.008
3.744 ± 0.01
6.114 ± 0.021
4.091 ± 0.016
6.058 ± 0.019
8.405 ± 0.025
5.995 ± 0.017
6.107 ± 0.017
1.466 ± 0.008
2.766 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here