Amino acid dipepetide frequency for Klebsiella phage vB_KpnM_BIS47

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.75AlaAla: 5.75 ± 0.502
0.916AlaCys: 0.916 ± 0.148
4.582AlaAsp: 4.582 ± 0.273
5.109AlaGlu: 5.109 ± 0.361
2.955AlaPhe: 2.955 ± 0.241
5.315AlaGly: 5.315 ± 0.413
1.168AlaHis: 1.168 ± 0.185
4.17AlaIle: 4.17 ± 0.292
5.155AlaLys: 5.155 ± 0.386
5.957AlaLeu: 5.957 ± 0.409
2.428AlaMet: 2.428 ± 0.236
3.093AlaAsn: 3.093 ± 0.285
2.337AlaPro: 2.337 ± 0.251
2.497AlaGln: 2.497 ± 0.262
3.757AlaArg: 3.757 ± 0.334
3.918AlaSer: 3.918 ± 0.283
4.33AlaThr: 4.33 ± 0.341
4.697AlaVal: 4.697 ± 0.315
1.214AlaTrp: 1.214 ± 0.168
2.841AlaTyr: 2.841 ± 0.24
0.0AlaXaa: 0.0 ± 0.0
Cys
0.779CysAla: 0.779 ± 0.148
0.229CysCys: 0.229 ± 0.069
0.893CysAsp: 0.893 ± 0.172
0.619CysGlu: 0.619 ± 0.129
0.596CysPhe: 0.596 ± 0.112
1.123CysGly: 1.123 ± 0.164
0.435CysHis: 0.435 ± 0.102
0.687CysIle: 0.687 ± 0.124
0.802CysLys: 0.802 ± 0.141
0.962CysLeu: 0.962 ± 0.139
0.321CysMet: 0.321 ± 0.091
0.481CysAsn: 0.481 ± 0.093
0.527CysPro: 0.527 ± 0.122
0.389CysGln: 0.389 ± 0.089
0.573CysArg: 0.573 ± 0.127
0.825CysSer: 0.825 ± 0.154
0.664CysThr: 0.664 ± 0.111
0.893CysVal: 0.893 ± 0.177
0.275CysTrp: 0.275 ± 0.073
0.687CysTyr: 0.687 ± 0.126
0.0CysXaa: 0.0 ± 0.0
Asp
3.986AspAla: 3.986 ± 0.355
0.802AspCys: 0.802 ± 0.127
4.193AspAsp: 4.193 ± 0.327
4.742AspGlu: 4.742 ± 0.314
2.887AspPhe: 2.887 ± 0.299
5.063AspGly: 5.063 ± 0.348
1.214AspHis: 1.214 ± 0.179
4.032AspIle: 4.032 ± 0.299
4.238AspLys: 4.238 ± 0.319
6.209AspLeu: 6.209 ± 0.364
1.764AspMet: 1.764 ± 0.266
3.184AspAsn: 3.184 ± 0.259
2.772AspPro: 2.772 ± 0.237
1.627AspGln: 1.627 ± 0.21
2.726AspArg: 2.726 ± 0.28
3.437AspSer: 3.437 ± 0.318
3.253AspThr: 3.253 ± 0.28
4.765AspVal: 4.765 ± 0.331
1.375AspTrp: 1.375 ± 0.168
2.68AspTyr: 2.68 ± 0.235
0.0AspXaa: 0.0 ± 0.0
Glu
5.75GluAla: 5.75 ± 0.336
0.733GluCys: 0.733 ± 0.134
3.918GluAsp: 3.918 ± 0.308
5.223GluGlu: 5.223 ± 0.398
2.818GluPhe: 2.818 ± 0.256
3.689GluGly: 3.689 ± 0.279
1.306GluHis: 1.306 ± 0.187
4.651GluIle: 4.651 ± 0.315
5.659GluLys: 5.659 ± 0.403
5.453GluLeu: 5.453 ± 0.327
2.176GluMet: 2.176 ± 0.259
3.414GluAsn: 3.414 ± 0.33
1.672GluPro: 1.672 ± 0.186
1.97GluGln: 1.97 ± 0.228
3.551GluArg: 3.551 ± 0.319
3.849GluSer: 3.849 ± 0.324
3.345GluThr: 3.345 ± 0.312
4.399GluVal: 4.399 ± 0.373
1.168GluTrp: 1.168 ± 0.158
2.795GluTyr: 2.795 ± 0.229
0.0GluXaa: 0.0 ± 0.0
Phe
2.658PheAla: 2.658 ± 0.259
0.71PheCys: 0.71 ± 0.133
3.139PheAsp: 3.139 ± 0.275
2.726PheGlu: 2.726 ± 0.289
1.81PhePhe: 1.81 ± 0.242
3.368PheGly: 3.368 ± 0.272
0.641PheHis: 0.641 ± 0.136
2.383PheIle: 2.383 ± 0.232
2.314PheLys: 2.314 ± 0.23
3.047PheLeu: 3.047 ± 0.284
1.123PheMet: 1.123 ± 0.165
2.428PheAsn: 2.428 ± 0.255
1.466PhePro: 1.466 ± 0.185
1.512PheGln: 1.512 ± 0.199
2.131PheArg: 2.131 ± 0.217
3.024PheSer: 3.024 ± 0.358
2.337PheThr: 2.337 ± 0.237
2.864PheVal: 2.864 ± 0.242
0.687PheTrp: 0.687 ± 0.122
1.604PheTyr: 1.604 ± 0.179
0.0PheXaa: 0.0 ± 0.0
Gly
4.238GlyAla: 4.238 ± 0.363
0.848GlyCys: 0.848 ± 0.133
4.399GlyAsp: 4.399 ± 0.339
4.49GlyGlu: 4.49 ± 0.31
3.322GlyPhe: 3.322 ± 0.304
4.742GlyGly: 4.742 ± 0.383
1.329GlyHis: 1.329 ± 0.164
4.055GlyIle: 4.055 ± 0.298
5.728GlyLys: 5.728 ± 0.361
5.155GlyLeu: 5.155 ± 0.377
2.245GlyMet: 2.245 ± 0.2
3.414GlyAsn: 3.414 ± 0.255
1.466GlyPro: 1.466 ± 0.17
2.176GlyGln: 2.176 ± 0.2
3.116GlyArg: 3.116 ± 0.268
3.872GlySer: 3.872 ± 0.326
3.78GlyThr: 3.78 ± 0.37
5.155GlyVal: 5.155 ± 0.291
1.489GlyTrp: 1.489 ± 0.198
3.322GlyTyr: 3.322 ± 0.273
0.0GlyXaa: 0.0 ± 0.0
His
0.962HisAla: 0.962 ± 0.136
0.298HisCys: 0.298 ± 0.082
1.26HisAsp: 1.26 ± 0.193
1.237HisGlu: 1.237 ± 0.187
1.008HisPhe: 1.008 ± 0.135
1.214HisGly: 1.214 ± 0.235
0.527HisHis: 0.527 ± 0.115
1.237HisIle: 1.237 ± 0.163
1.26HisLys: 1.26 ± 0.148
1.581HisLeu: 1.581 ± 0.199
0.504HisMet: 0.504 ± 0.094
1.008HisAsn: 1.008 ± 0.131
0.848HisPro: 0.848 ± 0.135
0.596HisGln: 0.596 ± 0.11
0.962HisArg: 0.962 ± 0.152
1.26HisSer: 1.26 ± 0.175
1.191HisThr: 1.191 ± 0.185
0.985HisVal: 0.985 ± 0.159
0.229HisTrp: 0.229 ± 0.07
0.893HisTyr: 0.893 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
4.445IleAla: 4.445 ± 0.328
0.779IleCys: 0.779 ± 0.14
3.803IleAsp: 3.803 ± 0.286
3.941IleGlu: 3.941 ± 0.268
2.176IlePhe: 2.176 ± 0.212
3.666IleGly: 3.666 ± 0.305
1.42IleHis: 1.42 ± 0.165
3.528IleIle: 3.528 ± 0.312
4.17IleLys: 4.17 ± 0.281
4.261IleLeu: 4.261 ± 0.315
1.764IleMet: 1.764 ± 0.197
2.474IleAsn: 2.474 ± 0.22
2.612IlePro: 2.612 ± 0.238
1.535IleGln: 1.535 ± 0.183
3.437IleArg: 3.437 ± 0.276
4.33IleSer: 4.33 ± 0.336
3.368IleThr: 3.368 ± 0.269
4.193IleVal: 4.193 ± 0.295
0.779IleTrp: 0.779 ± 0.148
2.474IleTyr: 2.474 ± 0.259
0.0IleXaa: 0.0 ± 0.0
Lys
5.819LysAla: 5.819 ± 0.507
0.641LysCys: 0.641 ± 0.121
4.971LysAsp: 4.971 ± 0.419
5.338LysGlu: 5.338 ± 0.386
2.841LysPhe: 2.841 ± 0.284
4.513LysGly: 4.513 ± 0.292
1.42LysHis: 1.42 ± 0.199
4.238LysIle: 4.238 ± 0.271
5.086LysLys: 5.086 ± 0.44
5.269LysLeu: 5.269 ± 0.334
2.222LysMet: 2.222 ± 0.229
3.116LysAsn: 3.116 ± 0.269
2.108LysPro: 2.108 ± 0.245
2.612LysGln: 2.612 ± 0.285
3.505LysArg: 3.505 ± 0.291
3.62LysSer: 3.62 ± 0.288
4.536LysThr: 4.536 ± 0.336
5.544LysVal: 5.544 ± 0.394
1.191LysTrp: 1.191 ± 0.163
2.91LysTyr: 2.91 ± 0.242
0.0LysXaa: 0.0 ± 0.0
Leu
6.438LeuAla: 6.438 ± 0.407
1.008LeuCys: 1.008 ± 0.162
5.659LeuAsp: 5.659 ± 0.343
5.842LeuGlu: 5.842 ± 0.361
2.726LeuPhe: 2.726 ± 0.273
4.422LeuGly: 4.422 ± 0.375
1.672LeuHis: 1.672 ± 0.223
3.986LeuIle: 3.986 ± 0.283
5.361LeuLys: 5.361 ± 0.369
6.094LeuLeu: 6.094 ± 0.408
2.291LeuMet: 2.291 ± 0.216
3.872LeuAsn: 3.872 ± 0.309
3.414LeuPro: 3.414 ± 0.289
2.451LeuGln: 2.451 ± 0.22
4.536LeuArg: 4.536 ± 0.348
5.659LeuSer: 5.659 ± 0.346
4.742LeuThr: 4.742 ± 0.393
4.811LeuVal: 4.811 ± 0.396
1.237LeuTrp: 1.237 ± 0.175
3.276LeuTyr: 3.276 ± 0.263
0.0LeuXaa: 0.0 ± 0.0
Met
2.497MetAla: 2.497 ± 0.268
0.412MetCys: 0.412 ± 0.126
1.191MetAsp: 1.191 ± 0.177
1.902MetGlu: 1.902 ± 0.22
1.375MetPhe: 1.375 ± 0.15
1.695MetGly: 1.695 ± 0.22
0.412MetHis: 0.412 ± 0.093
1.97MetIle: 1.97 ± 0.214
3.024MetLys: 3.024 ± 0.289
2.199MetLeu: 2.199 ± 0.212
1.1MetMet: 1.1 ± 0.173
1.535MetAsn: 1.535 ± 0.211
1.1MetPro: 1.1 ± 0.153
0.802MetGln: 0.802 ± 0.138
1.26MetArg: 1.26 ± 0.214
2.199MetSer: 2.199 ± 0.255
1.672MetThr: 1.672 ± 0.201
1.993MetVal: 1.993 ± 0.196
0.229MetTrp: 0.229 ± 0.065
0.916MetTyr: 0.916 ± 0.125
0.0MetXaa: 0.0 ± 0.0
Asn
3.368AsnAla: 3.368 ± 0.314
0.71AsnCys: 0.71 ± 0.134
2.68AsnAsp: 2.68 ± 0.278
2.062AsnGlu: 2.062 ± 0.22
2.154AsnPhe: 2.154 ± 0.243
4.147AsnGly: 4.147 ± 0.282
0.779AsnHis: 0.779 ± 0.136
2.978AsnIle: 2.978 ± 0.274
3.391AsnLys: 3.391 ± 0.291
3.574AsnLeu: 3.574 ± 0.279
1.489AsnMet: 1.489 ± 0.192
2.406AsnAsn: 2.406 ± 0.247
2.085AsnPro: 2.085 ± 0.214
1.466AsnGln: 1.466 ± 0.174
2.612AsnArg: 2.612 ± 0.242
2.91AsnSer: 2.91 ± 0.339
3.047AsnThr: 3.047 ± 0.321
3.139AsnVal: 3.139 ± 0.292
0.802AsnTrp: 0.802 ± 0.122
1.764AsnTyr: 1.764 ± 0.191
0.0AsnXaa: 0.0 ± 0.0
Pro
2.406ProAla: 2.406 ± 0.237
0.344ProCys: 0.344 ± 0.096
2.795ProAsp: 2.795 ± 0.284
3.139ProGlu: 3.139 ± 0.303
1.558ProPhe: 1.558 ± 0.185
2.428ProGly: 2.428 ± 0.246
0.733ProHis: 0.733 ± 0.144
1.489ProIle: 1.489 ± 0.154
2.131ProLys: 2.131 ± 0.232
2.749ProLeu: 2.749 ± 0.228
0.848ProMet: 0.848 ± 0.143
1.581ProAsn: 1.581 ± 0.165
1.054ProPro: 1.054 ± 0.178
1.512ProGln: 1.512 ± 0.158
1.489ProArg: 1.489 ± 0.2
2.062ProSer: 2.062 ± 0.239
2.085ProThr: 2.085 ± 0.239
2.887ProVal: 2.887 ± 0.217
0.527ProTrp: 0.527 ± 0.107
1.81ProTyr: 1.81 ± 0.199
0.0ProXaa: 0.0 ± 0.0
Gln
2.383GlnAla: 2.383 ± 0.201
0.367GlnCys: 0.367 ± 0.1
1.833GlnAsp: 1.833 ± 0.212
2.176GlnGlu: 2.176 ± 0.262
1.237GlnPhe: 1.237 ± 0.187
2.154GlnGly: 2.154 ± 0.181
0.412GlnHis: 0.412 ± 0.092
2.268GlnIle: 2.268 ± 0.253
2.245GlnLys: 2.245 ± 0.246
2.474GlnLeu: 2.474 ± 0.253
0.871GlnMet: 0.871 ± 0.151
1.81GlnAsn: 1.81 ± 0.193
0.893GlnPro: 0.893 ± 0.147
1.581GlnGln: 1.581 ± 0.215
1.375GlnArg: 1.375 ± 0.165
1.741GlnSer: 1.741 ± 0.213
1.902GlnThr: 1.902 ± 0.194
2.314GlnVal: 2.314 ± 0.226
0.596GlnTrp: 0.596 ± 0.119
1.306GlnTyr: 1.306 ± 0.172
0.0GlnXaa: 0.0 ± 0.0
Arg
3.139ArgAla: 3.139 ± 0.259
0.893ArgCys: 0.893 ± 0.15
3.528ArgAsp: 3.528 ± 0.316
3.207ArgGlu: 3.207 ± 0.256
1.718ArgPhe: 1.718 ± 0.205
3.459ArgGly: 3.459 ± 0.274
0.939ArgHis: 0.939 ± 0.153
3.116ArgIle: 3.116 ± 0.281
4.215ArgLys: 4.215 ± 0.349
3.734ArgLeu: 3.734 ± 0.262
1.42ArgMet: 1.42 ± 0.206
2.497ArgAsn: 2.497 ± 0.255
1.833ArgPro: 1.833 ± 0.202
1.947ArgGln: 1.947 ± 0.216
2.749ArgArg: 2.749 ± 0.294
3.001ArgSer: 3.001 ± 0.268
2.36ArgThr: 2.36 ± 0.221
3.276ArgVal: 3.276 ± 0.28
0.596ArgTrp: 0.596 ± 0.119
2.176ArgTyr: 2.176 ± 0.258
0.0ArgXaa: 0.0 ± 0.0
Ser
4.513SerAla: 4.513 ± 0.396
0.802SerCys: 0.802 ± 0.146
3.78SerAsp: 3.78 ± 0.265
3.666SerGlu: 3.666 ± 0.296
2.864SerPhe: 2.864 ± 0.212
4.651SerGly: 4.651 ± 0.39
1.008SerHis: 1.008 ± 0.147
3.482SerIle: 3.482 ± 0.254
3.849SerLys: 3.849 ± 0.308
4.994SerLeu: 4.994 ± 0.371
1.627SerMet: 1.627 ± 0.18
3.024SerAsn: 3.024 ± 0.265
2.131SerPro: 2.131 ± 0.191
1.741SerGln: 1.741 ± 0.188
3.207SerArg: 3.207 ± 0.27
3.849SerSer: 3.849 ± 0.361
3.689SerThr: 3.689 ± 0.371
4.605SerVal: 4.605 ± 0.37
1.146SerTrp: 1.146 ± 0.172
2.131SerTyr: 2.131 ± 0.249
0.0SerXaa: 0.0 ± 0.0
Thr
4.376ThrAla: 4.376 ± 0.346
0.619ThrCys: 0.619 ± 0.115
3.689ThrAsp: 3.689 ± 0.373
3.574ThrGlu: 3.574 ± 0.271
2.52ThrPhe: 2.52 ± 0.222
4.765ThrGly: 4.765 ± 0.398
1.146ThrHis: 1.146 ± 0.151
3.482ThrIle: 3.482 ± 0.285
4.009ThrLys: 4.009 ± 0.313
5.017ThrLeu: 5.017 ± 0.403
1.237ThrMet: 1.237 ± 0.163
2.062ThrAsn: 2.062 ± 0.256
2.978ThrPro: 2.978 ± 0.233
1.581ThrGln: 1.581 ± 0.178
2.406ThrArg: 2.406 ± 0.224
3.07ThrSer: 3.07 ± 0.268
3.643ThrThr: 3.643 ± 0.492
4.857ThrVal: 4.857 ± 0.378
1.123ThrTrp: 1.123 ± 0.173
2.268ThrTyr: 2.268 ± 0.227
0.0ThrXaa: 0.0 ± 0.0
Val
4.971ValAla: 4.971 ± 0.357
0.871ValCys: 0.871 ± 0.166
4.467ValAsp: 4.467 ± 0.331
4.926ValGlu: 4.926 ± 0.406
3.001ValPhe: 3.001 ± 0.236
4.467ValGly: 4.467 ± 0.303
1.054ValHis: 1.054 ± 0.163
4.513ValIle: 4.513 ± 0.305
4.903ValLys: 4.903 ± 0.402
5.361ValLeu: 5.361 ± 0.307
1.924ValMet: 1.924 ± 0.192
3.23ValAsn: 3.23 ± 0.336
2.268ValPro: 2.268 ± 0.269
1.993ValGln: 1.993 ± 0.264
3.459ValArg: 3.459 ± 0.262
4.651ValSer: 4.651 ± 0.393
4.674ValThr: 4.674 ± 0.375
5.934ValVal: 5.934 ± 0.488
1.191ValTrp: 1.191 ± 0.183
2.818ValTyr: 2.818 ± 0.235
0.0ValXaa: 0.0 ± 0.0
Trp
0.985TrpAla: 0.985 ± 0.147
0.229TrpCys: 0.229 ± 0.089
1.146TrpAsp: 1.146 ± 0.191
1.283TrpGlu: 1.283 ± 0.157
0.71TrpPhe: 0.71 ± 0.137
0.893TrpGly: 0.893 ± 0.168
0.275TrpHis: 0.275 ± 0.069
0.848TrpIle: 0.848 ± 0.148
1.146TrpLys: 1.146 ± 0.17
1.787TrpLeu: 1.787 ± 0.201
0.916TrpMet: 0.916 ± 0.149
0.848TrpAsn: 0.848 ± 0.15
0.412TrpPro: 0.412 ± 0.087
0.619TrpGln: 0.619 ± 0.104
0.664TrpArg: 0.664 ± 0.128
0.939TrpSer: 0.939 ± 0.153
0.939TrpThr: 0.939 ± 0.147
0.871TrpVal: 0.871 ± 0.133
0.458TrpTrp: 0.458 ± 0.104
1.008TrpTyr: 1.008 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.726TyrAla: 2.726 ± 0.232
0.55TyrCys: 0.55 ± 0.103
3.024TyrAsp: 3.024 ± 0.28
2.337TyrGlu: 2.337 ± 0.233
1.695TyrPhe: 1.695 ± 0.213
2.566TyrGly: 2.566 ± 0.245
1.168TyrHis: 1.168 ± 0.151
2.039TyrIle: 2.039 ± 0.209
2.841TyrLys: 2.841 ± 0.261
3.62TyrLeu: 3.62 ± 0.291
1.191TyrMet: 1.191 ± 0.192
2.085TyrAsn: 2.085 ± 0.242
1.65TyrPro: 1.65 ± 0.183
1.329TyrGln: 1.329 ± 0.165
2.291TyrArg: 2.291 ± 0.243
2.543TyrSer: 2.543 ± 0.248
2.864TyrThr: 2.864 ± 0.316
2.497TyrVal: 2.497 ± 0.243
0.664TyrTrp: 0.664 ± 0.117
2.108TyrTyr: 2.108 ± 0.246
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 262 proteins (43650 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski