Streptococcus phage P7573
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286QPL1|A0A286QPL1_9CAUD Tape measure protein OS=Streptococcus phage P7573 OX=1971429 GN=P7573_15 PE=4 SV=1
MM1 pKa = 7.12 VNWVDD6 pKa = 4.58 KK7 pKa = 11.1 DD8 pKa = 4.24 GNDD11 pKa = 3.97 LPDD14 pKa = 4.77 GADD17 pKa = 3.18 QDD19 pKa = 4.63 FKK21 pKa = 11.75 AGMFFSFAGDD31 pKa = 3.56 EE32 pKa = 4.15 NNITDD37 pKa = 3.82 TGEE40 pKa = 3.65 GGYY43 pKa = 10.61 YY44 pKa = 9.76 GGYY47 pKa = 8.0 YY48 pKa = 9.41 YY49 pKa = 10.76 RR50 pKa = 11.84 RR51 pKa = 11.84 FEE53 pKa = 4.06 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.567
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.795
Rodwell 3.617
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.656
EMBOSS 3.795
Sillero 3.923
Patrickios 0.477
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|A0A286QPM9|A0A286QPM9_9CAUD Uncharacterized protein OS=Streptococcus phage P7573 OX=1971429 GN=P7573_47 PE=4 SV=1
MM1 pKa = 7.84 ASFRR5 pKa = 11.84 LNSRR9 pKa = 11.84 TVTSTFSRR17 pKa = 11.84 GFLFLQKK24 pKa = 9.04 KK25 pKa = 7.7 TKK27 pKa = 9.94 FLNKK31 pKa = 10.08
Molecular weight: 3.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.284
IPC2_protein 10.628
IPC_protein 12.149
Toseland 12.34
ProMoST 12.808
Dawson 12.34
Bjellqvist 12.31
Wikipedia 12.793
Rodwell 12.281
Grimsley 12.369
Solomon 12.808
Lehninger 12.72
Nozaki 12.325
DTASelect 12.31
Thurlkill 12.325
EMBOSS 12.822
Sillero 12.325
Patrickios 12.047
IPC_peptide 12.822
IPC2_peptide 11.798
IPC2.peptide.svr19 9.016
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
11613
31
1602
241.9
27.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.054 ± 0.502
0.646 ± 0.172
6.467 ± 0.211
6.544 ± 0.442
4.159 ± 0.254
6.872 ± 0.563
1.438 ± 0.105
6.665 ± 0.25
8.542 ± 0.568
7.948 ± 0.409
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.506 ± 0.156
6.381 ± 0.254
3.022 ± 0.222
4.03 ± 0.188
4.4 ± 0.289
6.45 ± 0.361
6.226 ± 0.347
6.166 ± 0.195
1.455 ± 0.172
4.03 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here