Amino acid dipepetide frequency for Picea glauca (White spruce) (Pinus glauca)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.025AlaAla: 3.025 ± 0.107
1.535AlaCys: 1.535 ± 0.061
1.335AlaAsp: 1.335 ± 0.051
1.587AlaGlu: 1.587 ± 0.057
2.876AlaPhe: 2.876 ± 0.08
3.258AlaGly: 3.258 ± 0.096
1.403AlaHis: 1.403 ± 0.057
2.917AlaIle: 2.917 ± 0.089
1.699AlaLys: 1.699 ± 0.062
6.667AlaLeu: 6.667 ± 0.128
2.047AlaMet: 2.047 ± 0.076
1.48AlaAsn: 1.48 ± 0.054
3.144AlaPro: 3.144 ± 0.085
1.661AlaGln: 1.661 ± 0.069
3.622AlaArg: 3.622 ± 0.081
5.35AlaSer: 5.35 ± 0.121
2.787AlaThr: 2.787 ± 0.074
2.191AlaVal: 2.191 ± 0.065
0.878AlaTrp: 0.878 ± 0.04
2.006AlaTyr: 2.006 ± 0.065
0.002AlaXaa: 0.002 ± 0.002
Cys
0.982CysAla: 0.982 ± 0.047
0.911CysCys: 0.911 ± 0.046
0.524CysAsp: 0.524 ± 0.033
0.577CysGlu: 0.577 ± 0.031
1.575CysPhe: 1.575 ± 0.06
1.022CysGly: 1.022 ± 0.047
0.689CysHis: 0.689 ± 0.039
1.305CysIle: 1.305 ± 0.05
0.718CysLys: 0.718 ± 0.042
3.486CysLeu: 3.486 ± 0.093
0.951CysMet: 0.951 ± 0.044
0.746CysAsn: 0.746 ± 0.041
1.518CysPro: 1.518 ± 0.064
0.594CysGln: 0.594 ± 0.032
1.325CysArg: 1.325 ± 0.053
2.451CysSer: 2.451 ± 0.068
1.041CysThr: 1.041 ± 0.043
1.163CysVal: 1.163 ± 0.049
0.591CysTrp: 0.591 ± 0.032
0.963CysTyr: 0.963 ± 0.049
0.002CysXaa: 0.002 ± 0.002
Asp
1.693AspAla: 1.693 ± 0.058
0.624AspCys: 0.624 ± 0.035
1.124AspAsp: 1.124 ± 0.064
1.38AspGlu: 1.38 ± 0.057
1.199AspPhe: 1.199 ± 0.058
2.165AspGly: 2.165 ± 0.08
1.094AspHis: 1.094 ± 0.052
1.837AspIle: 1.837 ± 0.064
1.443AspLys: 1.443 ± 0.057
5.193AspLeu: 5.193 ± 0.13
1.478AspMet: 1.478 ± 0.052
0.933AspAsn: 0.933 ± 0.048
2.364AspPro: 2.364 ± 0.073
1.915AspGln: 1.915 ± 0.065
2.732AspArg: 2.732 ± 0.068
2.722AspSer: 2.722 ± 0.101
1.531AspThr: 1.531 ± 0.058
1.457AspVal: 1.457 ± 0.057
0.671AspTrp: 0.671 ± 0.039
1.035AspTyr: 1.035 ± 0.045
0.0AspXaa: 0.0 ± 0.0
Glu
2.114GluAla: 2.114 ± 0.068
0.693GluCys: 0.693 ± 0.037
1.516GluAsp: 1.516 ± 0.064
2.376GluGlu: 2.376 ± 0.089
1.053GluPhe: 1.053 ± 0.046
3.214GluGly: 3.214 ± 0.083
1.022GluHis: 1.022 ± 0.043
2.25GluIle: 2.25 ± 0.078
1.957GluLys: 1.957 ± 0.08
4.527GluLeu: 4.527 ± 0.107
1.813GluMet: 1.813 ± 0.063
1.327GluAsn: 1.327 ± 0.059
2.248GluPro: 2.248 ± 0.067
1.608GluGln: 1.608 ± 0.055
3.433GluArg: 3.433 ± 0.091
3.027GluSer: 3.027 ± 0.082
1.896GluThr: 1.896 ± 0.071
2.303GluVal: 2.303 ± 0.07
0.709GluTrp: 0.709 ± 0.038
0.961GluTyr: 0.961 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
1.933PheAla: 1.933 ± 0.065
1.012PheCys: 1.012 ± 0.049
1.527PheAsp: 1.527 ± 0.062
1.293PheGlu: 1.293 ± 0.059
2.531PhePhe: 2.531 ± 0.079
2.148PheGly: 2.148 ± 0.069
1.768PheHis: 1.768 ± 0.06
2.96PheIle: 2.96 ± 0.083
1.201PheLys: 1.201 ± 0.052
6.112PheLeu: 6.112 ± 0.106
1.445PheMet: 1.445 ± 0.049
1.295PheAsn: 1.295 ± 0.056
3.525PhePro: 3.525 ± 0.094
1.529PheGln: 1.529 ± 0.055
2.817PheArg: 2.817 ± 0.075
4.191PheSer: 4.191 ± 0.097
2.455PheThr: 2.455 ± 0.071
2.264PheVal: 2.264 ± 0.066
0.569PheTrp: 0.569 ± 0.034
1.661PheTyr: 1.661 ± 0.055
0.002PheXaa: 0.002 ± 0.002
Gly
2.923GlyAla: 2.923 ± 0.08
1.356GlyCys: 1.356 ± 0.057
1.695GlyAsp: 1.695 ± 0.059
2.504GlyGlu: 2.504 ± 0.063
2.606GlyPhe: 2.606 ± 0.08
4.212GlyGly: 4.212 ± 0.09
1.415GlyHis: 1.415 ± 0.051
3.612GlyIle: 3.612 ± 0.096
3.634GlyLys: 3.634 ± 0.093
6.271GlyLeu: 6.271 ± 0.12
2.636GlyMet: 2.636 ± 0.071
2.146GlyAsn: 2.146 ± 0.064
2.766GlyPro: 2.766 ± 0.071
2.163GlyGln: 2.163 ± 0.066
4.878GlyArg: 4.878 ± 0.109
5.571GlySer: 5.571 ± 0.124
3.458GlyThr: 3.458 ± 0.077
2.925GlyVal: 2.925 ± 0.083
1.488GlyTrp: 1.488 ± 0.063
2.307GlyTyr: 2.307 ± 0.065
0.002GlyXaa: 0.002 ± 0.002
His
1.38HisAla: 1.38 ± 0.057
0.62HisCys: 0.62 ± 0.039
0.691HisAsp: 0.691 ± 0.038
0.931HisGlu: 0.931 ± 0.049
1.466HisPhe: 1.466 ± 0.058
1.34HisGly: 1.34 ± 0.054
1.112HisHis: 1.112 ± 0.053
1.646HisIle: 1.646 ± 0.058
1.155HisLys: 1.155 ± 0.047
4.222HisLeu: 4.222 ± 0.095
1.037HisMet: 1.037 ± 0.043
0.921HisAsn: 0.921 ± 0.045
2.299HisPro: 2.299 ± 0.08
1.238HisGln: 1.238 ± 0.056
1.931HisArg: 1.931 ± 0.064
2.998HisSer: 2.998 ± 0.079
1.439HisThr: 1.439 ± 0.06
1.155HisVal: 1.155 ± 0.05
0.494HisTrp: 0.494 ± 0.029
1.083HisTyr: 1.083 ± 0.045
0.0HisXaa: 0.0 ± 0.0
Ile
2.683IleAla: 2.683 ± 0.078
1.394IleCys: 1.394 ± 0.057
2.6IleAsp: 2.6 ± 0.084
2.571IleGlu: 2.571 ± 0.076
2.191IlePhe: 2.191 ± 0.069
3.569IleGly: 3.569 ± 0.095
2.12IleHis: 2.12 ± 0.076
3.496IleIle: 3.496 ± 0.1
2.433IleLys: 2.433 ± 0.088
6.358IleLeu: 6.358 ± 0.121
1.844IleMet: 1.844 ± 0.059
2.153IleAsn: 2.153 ± 0.065
3.941IlePro: 3.941 ± 0.098
2.226IleGln: 2.226 ± 0.072
4.035IleArg: 4.035 ± 0.108
5.348IleSer: 5.348 ± 0.112
3.053IleThr: 3.053 ± 0.077
2.545IleVal: 2.545 ± 0.082
0.827IleTrp: 0.827 ± 0.042
3.027IleTyr: 3.027 ± 0.085
0.0IleXaa: 0.0 ± 0.0
Lys
2.441LysAla: 2.441 ± 0.078
0.839LysCys: 0.839 ± 0.04
1.624LysAsp: 1.624 ± 0.081
2.795LysGlu: 2.795 ± 0.081
0.955LysPhe: 0.955 ± 0.046
3.037LysGly: 3.037 ± 0.083
1.089LysHis: 1.089 ± 0.043
2.067LysIle: 2.067 ± 0.077
2.577LysLys: 2.577 ± 0.102
4.84LysLeu: 4.84 ± 0.118
1.543LysMet: 1.543 ± 0.057
1.4LysAsn: 1.4 ± 0.057
2.488LysPro: 2.488 ± 0.073
1.827LysGln: 1.827 ± 0.06
3.252LysArg: 3.252 ± 0.094
2.972LysSer: 2.972 ± 0.08
1.909LysThr: 1.909 ± 0.06
2.411LysVal: 2.411 ± 0.074
0.73LysTrp: 0.73 ± 0.039
1.124LysTyr: 1.124 ± 0.048
0.0LysXaa: 0.0 ± 0.0
Leu
7.448LeuAla: 7.448 ± 0.138
2.953LeuCys: 2.953 ± 0.097
5.492LeuAsp: 5.492 ± 0.122
4.773LeuGlu: 4.773 ± 0.098
6.234LeuPhe: 6.234 ± 0.138
7.669LeuGly: 7.669 ± 0.137
3.356LeuHis: 3.356 ± 0.09
6.62LeuIle: 6.62 ± 0.13
4.266LeuLys: 4.266 ± 0.1
21.145LeuLeu: 21.145 ± 0.362
4.801LeuMet: 4.801 ± 0.105
4.293LeuAsn: 4.293 ± 0.088
9.702LeuPro: 9.702 ± 0.188
3.586LeuGln: 3.586 ± 0.087
7.055LeuArg: 7.055 ± 0.129
11.137LeuSer: 11.137 ± 0.154
6.043LeuThr: 6.043 ± 0.112
8.07LeuVal: 8.07 ± 0.133
1.815LeuTrp: 1.815 ± 0.069
4.598LeuTyr: 4.598 ± 0.098
0.004LeuXaa: 0.004 ± 0.005
Met
2.161MetAla: 2.161 ± 0.057
0.646MetCys: 0.646 ± 0.036
2.232MetAsp: 2.232 ± 0.075
1.964MetGlu: 1.964 ± 0.062
1.1MetPhe: 1.1 ± 0.044
2.695MetGly: 2.695 ± 0.063
1.242MetHis: 1.242 ± 0.05
1.874MetIle: 1.874 ± 0.065
1.943MetLys: 1.943 ± 0.062
5.391MetLeu: 5.391 ± 0.13
1.276MetMet: 1.276 ± 0.06
1.791MetAsn: 1.791 ± 0.06
2.476MetPro: 2.476 ± 0.08
1.031MetGln: 1.031 ± 0.05
2.283MetArg: 2.283 ± 0.065
2.242MetSer: 2.242 ± 0.067
1.559MetThr: 1.559 ± 0.063
2.289MetVal: 2.289 ± 0.073
0.317MetTrp: 0.317 ± 0.026
0.953MetTyr: 0.953 ± 0.051
0.002MetXaa: 0.002 ± 0.002
Asn
1.65AsnAla: 1.65 ± 0.067
0.705AsnCys: 0.705 ± 0.041
1.063AsnAsp: 1.063 ± 0.049
1.714AsnGlu: 1.714 ± 0.061
0.892AsnPhe: 0.892 ± 0.046
1.844AsnGly: 1.844 ± 0.062
1.157AsnHis: 1.157 ± 0.05
1.905AsnIle: 1.905 ± 0.066
2.01AsnLys: 2.01 ± 0.064
4.273AsnLeu: 4.273 ± 0.101
1.362AsnMet: 1.362 ± 0.052
1.498AsnAsn: 1.498 ± 0.063
2.441AsnPro: 2.441 ± 0.071
1.888AsnGln: 1.888 ± 0.076
2.588AsnArg: 2.588 ± 0.08
2.799AsnSer: 2.799 ± 0.084
1.699AsnThr: 1.699 ± 0.061
1.376AsnVal: 1.376 ± 0.057
0.589AsnTrp: 0.589 ± 0.037
1.329AsnTyr: 1.329 ± 0.053
0.002AsnXaa: 0.002 ± 0.002
Pro
3.401ProAla: 3.401 ± 0.094
1.602ProCys: 1.602 ± 0.068
1.807ProAsp: 1.807 ± 0.066
2.088ProGlu: 2.088 ± 0.072
3.378ProPhe: 3.378 ± 0.093
3.372ProGly: 3.372 ± 0.086
1.925ProHis: 1.925 ± 0.069
4.134ProIle: 4.134 ± 0.1
2.163ProLys: 2.163 ± 0.055
8.801ProLeu: 8.801 ± 0.177
2.252ProMet: 2.252 ± 0.07
2.163ProAsn: 2.163 ± 0.067
4.655ProPro: 4.655 ± 0.121
1.683ProGln: 1.683 ± 0.064
3.681ProArg: 3.681 ± 0.08
6.854ProSer: 6.854 ± 0.134
3.986ProThr: 3.986 ± 0.107
3.39ProVal: 3.39 ± 0.092
0.839ProTrp: 0.839 ± 0.037
2.543ProTyr: 2.543 ± 0.075
0.002ProXaa: 0.002 ± 0.002
Gln
2.134GlnAla: 2.134 ± 0.071
0.685GlnCys: 0.685 ± 0.042
1.106GlnAsp: 1.106 ± 0.049
1.575GlnGlu: 1.575 ± 0.054
1.205GlnPhe: 1.205 ± 0.058
2.096GlnGly: 2.096 ± 0.072
0.878GlnHis: 0.878 ± 0.043
1.709GlnIle: 1.709 ± 0.066
1.482GlnLys: 1.482 ± 0.06
5.0GlnLeu: 5.0 ± 0.13
1.386GlnMet: 1.386 ± 0.059
1.167GlnAsn: 1.167 ± 0.052
2.421GlnPro: 2.421 ± 0.067
1.701GlnGln: 1.701 ± 0.06
2.392GlnArg: 2.392 ± 0.066
2.882GlnSer: 2.882 ± 0.083
1.579GlnThr: 1.579 ± 0.058
2.035GlnVal: 2.035 ± 0.068
0.539GlnTrp: 0.539 ± 0.033
0.86GlnTyr: 0.86 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
3.827ArgAla: 3.827 ± 0.089
1.677ArgCys: 1.677 ± 0.066
2.445ArgAsp: 2.445 ± 0.082
3.262ArgGlu: 3.262 ± 0.083
2.716ArgPhe: 2.716 ± 0.075
4.289ArgGly: 4.289 ± 0.101
1.449ArgHis: 1.449 ± 0.058
3.986ArgIle: 3.986 ± 0.098
3.754ArgLys: 3.754 ± 0.111
6.765ArgLeu: 6.765 ± 0.129
2.638ArgMet: 2.638 ± 0.077
2.646ArgAsn: 2.646 ± 0.078
3.382ArgPro: 3.382 ± 0.09
2.008ArgGln: 2.008 ± 0.065
4.764ArgArg: 4.764 ± 0.11
6.368ArgSer: 6.368 ± 0.127
3.628ArgThr: 3.628 ± 0.092
3.956ArgVal: 3.956 ± 0.09
1.218ArgTrp: 1.218 ± 0.055
2.256ArgTyr: 2.256 ± 0.061
0.0ArgXaa: 0.0 ± 0.0
Ser
4.077SerAla: 4.077 ± 0.091
2.203SerCys: 2.203 ± 0.071
2.396SerAsp: 2.396 ± 0.077
2.754SerGlu: 2.754 ± 0.078
5.202SerPhe: 5.202 ± 0.112
4.62SerGly: 4.62 ± 0.11
2.567SerHis: 2.567 ± 0.081
6.295SerIle: 6.295 ± 0.125
3.415SerLys: 3.415 ± 0.082
12.155SerLeu: 12.155 ± 0.167
2.986SerMet: 2.986 ± 0.08
3.171SerAsn: 3.171 ± 0.081
5.838SerPro: 5.838 ± 0.1
2.972SerGln: 2.972 ± 0.065
5.645SerArg: 5.645 ± 0.103
9.104SerSer: 9.104 ± 0.158
5.297SerThr: 5.297 ± 0.103
4.384SerVal: 4.384 ± 0.091
1.344SerTrp: 1.344 ± 0.056
3.399SerTyr: 3.399 ± 0.083
0.002SerXaa: 0.002 ± 0.002
Thr
2.555ThrAla: 2.555 ± 0.081
1.272ThrCys: 1.272 ± 0.051
1.616ThrAsp: 1.616 ± 0.062
1.945ThrGlu: 1.945 ± 0.066
2.319ThrPhe: 2.319 ± 0.074
3.338ThrGly: 3.338 ± 0.077
1.848ThrHis: 1.848 ± 0.074
2.947ThrIle: 2.947 ± 0.078
2.116ThrLys: 2.116 ± 0.066
6.086ThrLeu: 6.086 ± 0.11
1.903ThrMet: 1.903 ± 0.064
1.951ThrAsn: 1.951 ± 0.064
2.998ThrPro: 2.998 ± 0.08
1.709ThrGln: 1.709 ± 0.058
3.671ThrArg: 3.671 ± 0.095
4.685ThrSer: 4.685 ± 0.092
3.455ThrThr: 3.455 ± 0.108
1.955ThrVal: 1.955 ± 0.063
0.776ThrTrp: 0.776 ± 0.048
2.496ThrTyr: 2.496 ± 0.079
0.0ThrXaa: 0.0 ± 0.0
Val
2.392ValAla: 2.392 ± 0.074
1.175ValCys: 1.175 ± 0.052
2.016ValAsp: 2.016 ± 0.071
2.094ValGlu: 2.094 ± 0.073
2.175ValPhe: 2.175 ± 0.06
3.616ValGly: 3.616 ± 0.091
1.492ValHis: 1.492 ± 0.059
2.998ValIle: 2.998 ± 0.088
2.0ValLys: 2.0 ± 0.061
6.569ValLeu: 6.569 ± 0.121
1.945ValMet: 1.945 ± 0.063
1.831ValAsn: 1.831 ± 0.069
3.415ValPro: 3.415 ± 0.08
1.955ValGln: 1.955 ± 0.07
3.5ValArg: 3.5 ± 0.094
4.677ValSer: 4.677 ± 0.109
2.478ValThr: 2.478 ± 0.065
3.606ValVal: 3.606 ± 0.101
0.85ValTrp: 0.85 ± 0.04
1.728ValTyr: 1.728 ± 0.067
0.0ValXaa: 0.0 ± 0.0
Trp
0.89TrpAla: 0.89 ± 0.046
0.384TrpCys: 0.384 ± 0.031
0.657TrpAsp: 0.657 ± 0.037
0.734TrpGlu: 0.734 ± 0.039
0.803TrpPhe: 0.803 ± 0.042
1.012TrpGly: 1.012 ± 0.047
0.429TrpHis: 0.429 ± 0.03
1.216TrpIle: 1.216 ± 0.052
0.754TrpLys: 0.754 ± 0.036
2.059TrpLeu: 2.059 ± 0.076
0.77TrpMet: 0.77 ± 0.044
0.644TrpAsn: 0.644 ± 0.04
0.726TrpPro: 0.726 ± 0.04
0.333TrpGln: 0.333 ± 0.026
0.919TrpArg: 0.919 ± 0.041
1.183TrpSer: 1.183 ± 0.048
0.709TrpThr: 0.709 ± 0.044
1.254TrpVal: 1.254 ± 0.049
0.378TrpTrp: 0.378 ± 0.03
0.419TrpTyr: 0.419 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.76TyrAla: 1.76 ± 0.064
0.842TyrCys: 0.842 ± 0.037
1.122TyrAsp: 1.122 ± 0.049
1.132TyrGlu: 1.132 ± 0.048
1.762TyrPhe: 1.762 ± 0.056
2.053TyrGly: 2.053 ± 0.062
1.102TyrHis: 1.102 ± 0.044
2.638TyrIle: 2.638 ± 0.078
1.199TyrLys: 1.199 ± 0.051
5.132TyrLeu: 5.132 ± 0.118
1.35TyrMet: 1.35 ± 0.049
1.226TyrAsn: 1.226 ± 0.045
2.372TyrPro: 2.372 ± 0.072
1.205TyrGln: 1.205 ± 0.043
2.512TyrArg: 2.512 ± 0.071
3.266TyrSer: 3.266 ± 0.078
1.64TyrThr: 1.64 ± 0.055
1.783TyrVal: 1.783 ± 0.061
0.65TyrTrp: 0.65 ± 0.039
1.529TyrTyr: 1.529 ± 0.068
0.002TyrXaa: 0.002 ± 0.002
Xaa
0.0XaaAla: 0.0 ± 0.0
0.006XaaCys: 0.006 ± 0.005
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.008XaaLeu: 0.008 ± 0.007
0.0XaaMet: 0.0 ± 0.0
0.002XaaAsn: 0.002 ± 0.002
0.0XaaPro: 0.0 ± 0.0
0.002XaaGln: 0.002 ± 0.002
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.002XaaVal: 0.002 ± 0.002
0.0XaaTrp: 0.0 ± 0.0
0.002XaaTyr: 0.002 ± 0.002
0.004XaaXaa: 0.004 ± 0.005
Statistics based on 6155 proteins (491974 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski