Amino acid dipepetide frequency for Mycobacterium phage Rando14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.375AlaAla: 20.375 ± 1.544
1.246AlaCys: 1.246 ± 0.259
8.995AlaAsp: 8.995 ± 0.553
7.966AlaGlu: 7.966 ± 0.83
2.872AlaPhe: 2.872 ± 0.439
11.0AlaGly: 11.0 ± 0.92
2.547AlaHis: 2.547 ± 0.384
4.877AlaIle: 4.877 ± 0.423
4.335AlaLys: 4.335 ± 0.598
9.212AlaLeu: 9.212 ± 0.747
2.926AlaMet: 2.926 ± 0.461
2.438AlaAsn: 2.438 ± 0.35
4.985AlaPro: 4.985 ± 0.536
4.064AlaGln: 4.064 ± 0.499
8.562AlaArg: 8.562 ± 0.683
5.419AlaSer: 5.419 ± 0.594
7.912AlaThr: 7.912 ± 0.661
9.375AlaVal: 9.375 ± 0.706
2.276AlaTrp: 2.276 ± 0.359
2.276AlaTyr: 2.276 ± 0.322
0.0AlaXaa: 0.0 ± 0.0
Cys
1.734CysAla: 1.734 ± 0.379
0.217CysCys: 0.217 ± 0.106
0.759CysAsp: 0.759 ± 0.22
0.434CysGlu: 0.434 ± 0.158
0.108CysPhe: 0.108 ± 0.081
1.68CysGly: 1.68 ± 0.41
0.542CysHis: 0.542 ± 0.207
0.542CysIle: 0.542 ± 0.164
0.379CysLys: 0.379 ± 0.16
0.271CysLeu: 0.271 ± 0.118
0.163CysMet: 0.163 ± 0.095
0.488CysAsn: 0.488 ± 0.167
0.65CysPro: 0.65 ± 0.182
0.379CysGln: 0.379 ± 0.144
0.596CysArg: 0.596 ± 0.162
0.488CysSer: 0.488 ± 0.181
0.542CysThr: 0.542 ± 0.185
0.867CysVal: 0.867 ± 0.22
0.379CysTrp: 0.379 ± 0.136
0.163CysTyr: 0.163 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
8.508AspAla: 8.508 ± 0.714
1.03AspCys: 1.03 ± 0.29
5.148AspAsp: 5.148 ± 0.682
4.931AspGlu: 4.931 ± 0.565
1.084AspPhe: 1.084 ± 0.234
6.448AspGly: 6.448 ± 0.522
1.138AspHis: 1.138 ± 0.241
2.384AspIle: 2.384 ± 0.324
1.571AspLys: 1.571 ± 0.262
6.34AspLeu: 6.34 ± 0.489
1.192AspMet: 1.192 ± 0.315
1.68AspAsn: 1.68 ± 0.248
3.902AspPro: 3.902 ± 0.473
1.517AspGln: 1.517 ± 0.273
5.69AspArg: 5.69 ± 0.57
1.951AspSer: 1.951 ± 0.3
3.631AspThr: 3.631 ± 0.349
4.985AspVal: 4.985 ± 0.407
0.921AspTrp: 0.921 ± 0.205
1.463AspTyr: 1.463 ± 0.298
0.0AspXaa: 0.0 ± 0.0
Glu
6.882GluAla: 6.882 ± 0.74
0.704GluCys: 0.704 ± 0.215
2.601GluAsp: 2.601 ± 0.415
2.276GluGlu: 2.276 ± 0.32
1.897GluPhe: 1.897 ± 0.352
3.306GluGly: 3.306 ± 0.47
1.842GluHis: 1.842 ± 0.371
1.409GluIle: 1.409 ± 0.291
1.626GluLys: 1.626 ± 0.294
5.527GluLeu: 5.527 ± 0.475
1.734GluMet: 1.734 ± 0.221
1.138GluAsn: 1.138 ± 0.238
3.197GluPro: 3.197 ± 0.46
2.926GluGln: 2.926 ± 0.534
5.581GluArg: 5.581 ± 0.659
2.818GluSer: 2.818 ± 0.324
2.818GluThr: 2.818 ± 0.392
4.227GluVal: 4.227 ± 0.44
0.65GluTrp: 0.65 ± 0.152
1.68GluTyr: 1.68 ± 0.239
0.0GluXaa: 0.0 ± 0.0
Phe
2.98PheAla: 2.98 ± 0.416
0.217PheCys: 0.217 ± 0.112
2.655PheAsp: 2.655 ± 0.355
1.571PheGlu: 1.571 ± 0.283
0.65PhePhe: 0.65 ± 0.156
3.089PheGly: 3.089 ± 0.425
0.271PheHis: 0.271 ± 0.12
1.192PheIle: 1.192 ± 0.246
1.138PheLys: 1.138 ± 0.285
1.951PheLeu: 1.951 ± 0.349
0.434PheMet: 0.434 ± 0.172
1.03PheAsn: 1.03 ± 0.22
1.355PhePro: 1.355 ± 0.254
0.759PheGln: 0.759 ± 0.179
1.842PheArg: 1.842 ± 0.267
1.409PheSer: 1.409 ± 0.251
1.734PheThr: 1.734 ± 0.25
1.463PheVal: 1.463 ± 0.22
0.217PheTrp: 0.217 ± 0.096
0.488PheTyr: 0.488 ± 0.165
0.0PheXaa: 0.0 ± 0.0
Gly
8.453GlyAla: 8.453 ± 0.836
1.463GlyCys: 1.463 ± 0.327
6.069GlyAsp: 6.069 ± 0.524
4.606GlyGlu: 4.606 ± 0.412
2.601GlyPhe: 2.601 ± 0.362
10.729GlyGly: 10.729 ± 1.67
2.005GlyHis: 2.005 ± 0.433
3.902GlyIle: 3.902 ± 0.466
3.36GlyLys: 3.36 ± 0.416
5.798GlyLeu: 5.798 ± 0.709
1.897GlyMet: 1.897 ± 0.368
3.793GlyAsn: 3.793 ± 0.357
3.793GlyPro: 3.793 ± 0.375
3.035GlyGln: 3.035 ± 0.521
5.961GlyArg: 5.961 ± 0.549
5.094GlySer: 5.094 ± 0.772
6.178GlyThr: 6.178 ± 0.515
6.882GlyVal: 6.882 ± 0.644
1.788GlyTrp: 1.788 ± 0.242
2.384GlyTyr: 2.384 ± 0.365
0.0GlyXaa: 0.0 ± 0.0
His
2.547HisAla: 2.547 ± 0.419
0.488HisCys: 0.488 ± 0.168
1.463HisAsp: 1.463 ± 0.335
1.246HisGlu: 1.246 ± 0.271
0.596HisPhe: 0.596 ± 0.202
2.276HisGly: 2.276 ± 0.417
0.867HisHis: 0.867 ± 0.275
0.704HisIle: 0.704 ± 0.192
0.65HisLys: 0.65 ± 0.228
2.059HisLeu: 2.059 ± 0.321
0.434HisMet: 0.434 ± 0.127
0.596HisAsn: 0.596 ± 0.15
1.192HisPro: 1.192 ± 0.322
0.65HisGln: 0.65 ± 0.208
2.222HisArg: 2.222 ± 0.586
0.813HisSer: 0.813 ± 0.2
1.626HisThr: 1.626 ± 0.309
1.68HisVal: 1.68 ± 0.291
0.379HisTrp: 0.379 ± 0.155
0.542HisTyr: 0.542 ± 0.181
0.0HisXaa: 0.0 ± 0.0
Ile
5.419IleAla: 5.419 ± 0.463
0.217IleCys: 0.217 ± 0.106
3.36IleAsp: 3.36 ± 0.397
2.709IleGlu: 2.709 ± 0.395
0.813IlePhe: 0.813 ± 0.191
4.281IleGly: 4.281 ± 0.61
0.379IleHis: 0.379 ± 0.125
1.301IleIle: 1.301 ± 0.246
1.517IleLys: 1.517 ± 0.309
2.709IleLeu: 2.709 ± 0.36
0.542IleMet: 0.542 ± 0.161
1.842IleAsn: 1.842 ± 0.333
2.926IlePro: 2.926 ± 0.465
1.138IleGln: 1.138 ± 0.234
2.709IleArg: 2.709 ± 0.368
2.168IleSer: 2.168 ± 0.421
3.143IleThr: 3.143 ± 0.445
2.98IleVal: 2.98 ± 0.357
0.596IleTrp: 0.596 ± 0.232
0.759IleTyr: 0.759 ± 0.167
0.0IleXaa: 0.0 ± 0.0
Lys
3.956LysAla: 3.956 ± 0.532
0.271LysCys: 0.271 ± 0.134
0.921LysAsp: 0.921 ± 0.251
0.975LysGlu: 0.975 ± 0.272
1.084LysPhe: 1.084 ± 0.258
2.655LysGly: 2.655 ± 0.356
0.813LysHis: 0.813 ± 0.221
1.246LysIle: 1.246 ± 0.279
0.813LysLys: 0.813 ± 0.198
3.793LysLeu: 3.793 ± 0.43
0.813LysMet: 0.813 ± 0.198
1.246LysAsn: 1.246 ± 0.297
2.547LysPro: 2.547 ± 0.355
1.355LysGln: 1.355 ± 0.304
3.306LysArg: 3.306 ± 0.506
1.626LysSer: 1.626 ± 0.325
1.788LysThr: 1.788 ± 0.33
2.384LysVal: 2.384 ± 0.368
0.867LysTrp: 0.867 ± 0.194
0.975LysTyr: 0.975 ± 0.244
0.0LysXaa: 0.0 ± 0.0
Leu
10.079LeuAla: 10.079 ± 0.749
1.301LeuCys: 1.301 ± 0.276
5.69LeuAsp: 5.69 ± 0.493
3.251LeuGlu: 3.251 ± 0.396
2.276LeuPhe: 2.276 ± 0.319
6.503LeuGly: 6.503 ± 0.517
2.005LeuHis: 2.005 ± 0.403
3.251LeuIle: 3.251 ± 0.432
2.98LeuLys: 2.98 ± 0.391
5.04LeuLeu: 5.04 ± 0.501
1.463LeuMet: 1.463 ± 0.242
2.005LeuAsn: 2.005 ± 0.432
3.739LeuPro: 3.739 ± 0.426
3.143LeuGln: 3.143 ± 0.46
6.774LeuArg: 6.774 ± 0.847
4.443LeuSer: 4.443 ± 0.5
5.527LeuThr: 5.527 ± 0.582
5.419LeuVal: 5.419 ± 0.505
1.734LeuTrp: 1.734 ± 0.285
1.463LeuTyr: 1.463 ± 0.245
0.0LeuXaa: 0.0 ± 0.0
Met
1.951MetAla: 1.951 ± 0.258
0.163MetCys: 0.163 ± 0.085
0.867MetAsp: 0.867 ± 0.199
0.434MetGlu: 0.434 ± 0.129
0.65MetPhe: 0.65 ± 0.184
1.734MetGly: 1.734 ± 0.335
0.325MetHis: 0.325 ± 0.131
1.03MetIle: 1.03 ± 0.239
0.596MetLys: 0.596 ± 0.213
2.059MetLeu: 2.059 ± 0.327
0.542MetMet: 0.542 ± 0.168
0.759MetAsn: 0.759 ± 0.2
1.138MetPro: 1.138 ± 0.196
0.596MetGln: 0.596 ± 0.219
1.842MetArg: 1.842 ± 0.323
2.438MetSer: 2.438 ± 0.454
2.059MetThr: 2.059 ± 0.304
1.788MetVal: 1.788 ± 0.299
0.325MetTrp: 0.325 ± 0.131
0.488MetTyr: 0.488 ± 0.189
0.0MetXaa: 0.0 ± 0.0
Asn
3.522AsnAla: 3.522 ± 0.606
0.217AsnCys: 0.217 ± 0.103
1.897AsnAsp: 1.897 ± 0.316
1.192AsnGlu: 1.192 ± 0.244
0.596AsnPhe: 0.596 ± 0.142
3.522AsnGly: 3.522 ± 0.546
0.704AsnHis: 0.704 ± 0.238
1.355AsnIle: 1.355 ± 0.298
1.138AsnLys: 1.138 ± 0.23
2.005AsnLeu: 2.005 ± 0.335
0.542AsnMet: 0.542 ± 0.155
0.813AsnAsn: 0.813 ± 0.212
3.035AsnPro: 3.035 ± 0.361
0.759AsnGln: 0.759 ± 0.186
1.951AsnArg: 1.951 ± 0.299
1.301AsnSer: 1.301 ± 0.266
1.246AsnThr: 1.246 ± 0.29
2.168AsnVal: 2.168 ± 0.292
0.813AsnTrp: 0.813 ± 0.17
0.542AsnTyr: 0.542 ± 0.167
0.0AsnXaa: 0.0 ± 0.0
Pro
6.719ProAla: 6.719 ± 0.496
0.325ProCys: 0.325 ± 0.205
3.793ProAsp: 3.793 ± 0.487
3.522ProGlu: 3.522 ± 0.383
1.301ProPhe: 1.301 ± 0.259
4.714ProGly: 4.714 ± 0.441
1.463ProHis: 1.463 ± 0.305
2.059ProIle: 2.059 ± 0.288
2.222ProLys: 2.222 ± 0.47
3.522ProLeu: 3.522 ± 0.51
1.355ProMet: 1.355 ± 0.236
1.626ProAsn: 1.626 ± 0.305
3.685ProPro: 3.685 ± 0.518
2.059ProGln: 2.059 ± 0.377
3.468ProArg: 3.468 ± 0.492
3.251ProSer: 3.251 ± 0.432
3.739ProThr: 3.739 ± 0.417
4.01ProVal: 4.01 ± 0.468
0.867ProTrp: 0.867 ± 0.211
1.192ProTyr: 1.192 ± 0.233
0.0ProXaa: 0.0 ± 0.0
Gln
4.931GlnAla: 4.931 ± 0.523
0.379GlnCys: 0.379 ± 0.15
1.409GlnAsp: 1.409 ± 0.198
1.355GlnGlu: 1.355 ± 0.257
1.03GlnPhe: 1.03 ± 0.261
1.626GlnGly: 1.626 ± 0.236
0.813GlnHis: 0.813 ± 0.203
2.113GlnIle: 2.113 ± 0.333
1.084GlnLys: 1.084 ± 0.247
2.709GlnLeu: 2.709 ± 0.462
0.921GlnMet: 0.921 ± 0.212
0.813GlnAsn: 0.813 ± 0.205
2.113GlnPro: 2.113 ± 0.289
1.192GlnGln: 1.192 ± 0.287
3.522GlnArg: 3.522 ± 0.427
1.517GlnSer: 1.517 ± 0.302
1.734GlnThr: 1.734 ± 0.303
2.547GlnVal: 2.547 ± 0.337
1.192GlnTrp: 1.192 ± 0.264
0.921GlnTyr: 0.921 ± 0.204
0.0GlnXaa: 0.0 ± 0.0
Arg
8.074ArgAla: 8.074 ± 0.94
0.975ArgCys: 0.975 ± 0.227
4.714ArgAsp: 4.714 ± 0.398
4.931ArgGlu: 4.931 ± 0.491
1.951ArgPhe: 1.951 ± 0.309
6.232ArgGly: 6.232 ± 0.716
2.168ArgHis: 2.168 ± 0.426
4.443ArgIle: 4.443 ± 0.369
3.251ArgLys: 3.251 ± 0.461
6.178ArgLeu: 6.178 ± 0.576
1.788ArgMet: 1.788 ± 0.255
2.493ArgAsn: 2.493 ± 0.323
3.197ArgPro: 3.197 ± 0.33
3.522ArgGln: 3.522 ± 0.425
8.074ArgArg: 8.074 ± 1.311
3.847ArgSer: 3.847 ± 0.404
3.847ArgThr: 3.847 ± 0.397
5.798ArgVal: 5.798 ± 0.507
1.68ArgTrp: 1.68 ± 0.336
2.384ArgTyr: 2.384 ± 0.406
0.0ArgXaa: 0.0 ± 0.0
Ser
6.232SerAla: 6.232 ± 0.76
0.434SerCys: 0.434 ± 0.167
2.764SerAsp: 2.764 ± 0.348
2.98SerGlu: 2.98 ± 0.423
1.517SerPhe: 1.517 ± 0.205
4.606SerGly: 4.606 ± 0.854
0.704SerHis: 0.704 ± 0.203
2.872SerIle: 2.872 ± 0.31
1.897SerLys: 1.897 ± 0.344
3.089SerLeu: 3.089 ± 0.444
1.788SerMet: 1.788 ± 0.267
1.842SerAsn: 1.842 ± 0.302
3.143SerPro: 3.143 ± 0.443
1.409SerGln: 1.409 ± 0.352
3.089SerArg: 3.089 ± 0.402
2.438SerSer: 2.438 ± 0.422
3.631SerThr: 3.631 ± 0.433
4.443SerVal: 4.443 ± 0.516
1.246SerTrp: 1.246 ± 0.3
1.084SerTyr: 1.084 ± 0.256
0.0SerXaa: 0.0 ± 0.0
Thr
7.803ThrAla: 7.803 ± 0.823
0.542ThrCys: 0.542 ± 0.194
3.468ThrAsp: 3.468 ± 0.508
3.576ThrGlu: 3.576 ± 0.404
2.222ThrPhe: 2.222 ± 0.255
6.286ThrGly: 6.286 ± 0.61
1.246ThrHis: 1.246 ± 0.296
2.601ThrIle: 2.601 ± 0.341
1.788ThrLys: 1.788 ± 0.347
4.714ThrLeu: 4.714 ± 0.521
1.138ThrMet: 1.138 ± 0.238
1.463ThrAsn: 1.463 ± 0.285
3.956ThrPro: 3.956 ± 0.519
1.463ThrGln: 1.463 ± 0.314
4.66ThrArg: 4.66 ± 0.574
3.251ThrSer: 3.251 ± 0.494
3.902ThrThr: 3.902 ± 0.455
6.286ThrVal: 6.286 ± 0.617
1.355ThrTrp: 1.355 ± 0.239
1.301ThrTyr: 1.301 ± 0.258
0.0ThrXaa: 0.0 ± 0.0
Val
8.995ValAla: 8.995 ± 1.02
0.704ValCys: 0.704 ± 0.234
6.503ValAsp: 6.503 ± 0.536
4.443ValGlu: 4.443 ± 0.671
2.059ValPhe: 2.059 ± 0.299
6.123ValGly: 6.123 ± 0.598
2.059ValHis: 2.059 ± 0.346
2.547ValIle: 2.547 ± 0.377
2.33ValLys: 2.33 ± 0.355
6.882ValLeu: 6.882 ± 0.615
1.463ValMet: 1.463 ± 0.225
1.951ValAsn: 1.951 ± 0.223
3.902ValPro: 3.902 ± 0.417
2.33ValGln: 2.33 ± 0.401
5.311ValArg: 5.311 ± 0.517
4.389ValSer: 4.389 ± 0.474
5.148ValThr: 5.148 ± 0.495
5.798ValVal: 5.798 ± 0.651
1.463ValTrp: 1.463 ± 0.231
1.734ValTyr: 1.734 ± 0.33
0.0ValXaa: 0.0 ± 0.0
Trp
1.951TrpAla: 1.951 ± 0.314
0.325TrpCys: 0.325 ± 0.124
1.03TrpAsp: 1.03 ± 0.311
0.867TrpGlu: 0.867 ± 0.232
0.65TrpPhe: 0.65 ± 0.175
0.921TrpGly: 0.921 ± 0.26
0.596TrpHis: 0.596 ± 0.198
0.867TrpIle: 0.867 ± 0.226
0.379TrpLys: 0.379 ± 0.146
2.33TrpLeu: 2.33 ± 0.401
0.271TrpMet: 0.271 ± 0.139
0.65TrpAsn: 0.65 ± 0.183
1.138TrpPro: 1.138 ± 0.265
0.813TrpGln: 0.813 ± 0.219
2.168TrpArg: 2.168 ± 0.317
1.246TrpSer: 1.246 ± 0.244
1.246TrpThr: 1.246 ± 0.226
1.355TrpVal: 1.355 ± 0.243
0.325TrpTrp: 0.325 ± 0.15
0.488TrpTyr: 0.488 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.601TyrAla: 2.601 ± 0.346
0.163TyrCys: 0.163 ± 0.094
1.409TyrAsp: 1.409 ± 0.3
1.571TyrGlu: 1.571 ± 0.271
0.488TyrPhe: 0.488 ± 0.155
2.059TyrGly: 2.059 ± 0.279
0.542TyrHis: 0.542 ± 0.139
0.813TyrIle: 0.813 ± 0.195
0.379TyrLys: 0.379 ± 0.169
1.897TyrLeu: 1.897 ± 0.319
0.325TyrMet: 0.325 ± 0.124
0.65TyrAsn: 0.65 ± 0.19
1.301TyrPro: 1.301 ± 0.271
0.813TyrGln: 0.813 ± 0.215
2.222TyrArg: 2.222 ± 0.328
1.301TyrSer: 1.301 ± 0.219
1.571TyrThr: 1.571 ± 0.325
1.68TyrVal: 1.68 ± 0.364
0.542TyrTrp: 0.542 ± 0.204
0.542TyrTyr: 0.542 ± 0.238
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (18455 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski