gamma proteobacterium HTCC5015

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2449 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5JUG5|B5JUG5_9GAMM Transposase OS=gamma proteobacterium HTCC5015 OX=391615 GN=GP5015_1446 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.49KK3 pKa = 10.01EE4 pKa = 3.88FARR7 pKa = 11.84SGKK10 pKa = 9.57HH11 pKa = 4.98VFMSLLLLLCYY22 pKa = 10.41SQAATANSTGISGYY36 pKa = 10.01SGEE39 pKa = 4.68SGSSCVSCHH48 pKa = 6.08NGNVYY53 pKa = 10.67NYY55 pKa = 9.91NSVISGDD62 pKa = 3.6STLTPNSSSSYY73 pKa = 8.3TFSLNRR79 pKa = 11.84VAGSTAGAAGFNLSADD95 pKa = 3.66NGTLSSGSGSKK106 pKa = 9.85ILNSEE111 pKa = 4.16LTHH114 pKa = 6.12SAKK117 pKa = 9.2KK118 pKa = 9.02TSNFNWSFNWTAPSADD134 pKa = 3.17GVYY137 pKa = 9.87TLYY140 pKa = 11.2ACGNPVDD147 pKa = 3.98NDD149 pKa = 3.82GVGLSDD155 pKa = 4.21TGDD158 pKa = 3.58GPADD162 pKa = 3.43CTEE165 pKa = 3.96FDD167 pKa = 3.6ITVNTPPTANSDD179 pKa = 3.68SSSLSEE185 pKa = 4.83DD186 pKa = 2.94GSYY189 pKa = 11.0DD190 pKa = 3.46YY191 pKa = 11.24VYY193 pKa = 10.5PLSNDD198 pKa = 2.86SSGDD202 pKa = 3.55VGDD205 pKa = 3.93TKK207 pKa = 11.38SLIGVCATSTLDD219 pKa = 3.49YY220 pKa = 10.09YY221 pKa = 11.6CNSTSYY227 pKa = 10.44TPSQGSFYY235 pKa = 11.09VAGSYY240 pKa = 9.2VRR242 pKa = 11.84YY243 pKa = 9.79LPAANYY249 pKa = 10.53NGTFSIKK256 pKa = 10.77YY257 pKa = 9.91KK258 pKa = 9.75MQDD261 pKa = 2.42SYY263 pKa = 12.16GDD265 pKa = 3.25VDD267 pKa = 3.96TANITYY273 pKa = 8.29TVSSVNDD280 pKa = 3.72DD281 pKa = 3.81PVVSGVDD288 pKa = 3.23TTLSYY293 pKa = 11.38TEE295 pKa = 4.11GQGARR300 pKa = 11.84TLEE303 pKa = 4.1SSINLSDD310 pKa = 4.19VDD312 pKa = 3.71HH313 pKa = 6.43TQLNRR318 pKa = 11.84AEE320 pKa = 4.27VEE322 pKa = 3.57ISGNYY327 pKa = 9.28VSGQDD332 pKa = 3.24VLAASNCSGLSCSFNSTNGTLTLFGTASIATYY364 pKa = 10.69QNALEE369 pKa = 4.32SVTYY373 pKa = 10.82ANTSEE378 pKa = 4.53DD379 pKa = 3.69PSTATRR385 pKa = 11.84TVRR388 pKa = 11.84IRR390 pKa = 11.84VRR392 pKa = 11.84DD393 pKa = 3.4TAYY396 pKa = 10.46AYY398 pKa = 11.13SNYY401 pKa = 9.12DD402 pKa = 3.1TMNITVSAEE411 pKa = 3.26NDD413 pKa = 3.4APVVSGFDD421 pKa = 3.07TTLNYY426 pKa = 9.95TEE428 pKa = 4.73NDD430 pKa = 3.53GARR433 pKa = 11.84SLEE436 pKa = 4.24SSVSLNDD443 pKa = 3.39IDD445 pKa = 5.35SSQLNRR451 pKa = 11.84AEE453 pKa = 3.73IQIYY457 pKa = 9.59SGYY460 pKa = 10.44INGEE464 pKa = 4.1DD465 pKa = 3.3VLAAGNCSALSCSFNTGTGTYY486 pKa = 9.46VFSGNASLATYY497 pKa = 8.92EE498 pKa = 4.25AVIEE502 pKa = 4.27SLTYY506 pKa = 9.86TNQSEE511 pKa = 4.55APSTSPRR518 pKa = 11.84AVRR521 pKa = 11.84LRR523 pKa = 11.84VRR525 pKa = 11.84DD526 pKa = 3.51TDD528 pKa = 3.45NDD530 pKa = 3.68YY531 pKa = 11.82SNGDD535 pKa = 3.74TLSIAVTAVEE545 pKa = 5.0DD546 pKa = 3.94PADD549 pKa = 3.96FDD551 pKa = 4.32SPVYY555 pKa = 10.44EE556 pKa = 4.33RR557 pKa = 11.84IGVGTSSSTTQNIAEE572 pKa = 4.53DD573 pKa = 3.85NQLQLQFAANDD584 pKa = 3.91PEE586 pKa = 4.62GVSTEE591 pKa = 4.04FNLVSTNPVLTGPEE605 pKa = 3.94PLISVSSDD613 pKa = 2.65GLLSWTPDD621 pKa = 3.06GTRR624 pKa = 11.84SSVQAVIGVNDD635 pKa = 4.63NIGSSPDD642 pKa = 3.22NTFSITLTVTGSNDD656 pKa = 3.03APVITEE662 pKa = 4.01GASINVNMDD671 pKa = 2.9EE672 pKa = 4.78DD673 pKa = 4.16ASPTAFNLTLNASDD687 pKa = 5.33LEE689 pKa = 4.29NDD691 pKa = 3.44TLTWSIVSQASKK703 pKa = 9.52GTASTSGTGNSKK715 pKa = 10.91AILYY719 pKa = 8.82TPDD722 pKa = 3.46LNEE725 pKa = 4.32NGSDD729 pKa = 3.26AFVVRR734 pKa = 11.84VTDD737 pKa = 4.42GSLSDD742 pKa = 5.46DD743 pKa = 3.0ITVNVNIAPQADD755 pKa = 3.67APVITDD761 pKa = 3.59GKK763 pKa = 10.12AITVAMSEE771 pKa = 4.69DD772 pKa = 3.76GSPTPFEE779 pKa = 4.35LVLNATDD786 pKa = 4.28PDD788 pKa = 4.44AGDD791 pKa = 3.52TLTWRR796 pKa = 11.84ISQAASRR803 pKa = 11.84GATSIPGATRR813 pKa = 11.84AGQPIVYY820 pKa = 9.44KK821 pKa = 10.91PNDD824 pKa = 3.81DD825 pKa = 3.97YY826 pKa = 11.65FGSDD830 pKa = 2.85QFTVSVSDD838 pKa = 3.64GRR840 pKa = 11.84LSDD843 pKa = 4.49SIDD846 pKa = 3.27VTVNIAPVNDD856 pKa = 3.47NPVIQTIGTLPAATEE871 pKa = 3.85DD872 pKa = 3.28QLYY875 pKa = 9.49QFVVAVSDD883 pKa = 3.68VDD885 pKa = 3.65HH886 pKa = 6.82SVPEE890 pKa = 4.14DD891 pKa = 3.68LRR893 pKa = 11.84FSLNNAPDD901 pKa = 3.56GMQINATGQVSWTPSEE917 pKa = 4.23GVSGSGKK924 pKa = 9.27VGVRR928 pKa = 11.84VEE930 pKa = 4.5DD931 pKa = 3.48SAGGIDD937 pKa = 3.2AAMFSVSVSAVNDD950 pKa = 4.22GPSINSTPPNTATEE964 pKa = 4.42GEE966 pKa = 4.4LYY968 pKa = 10.22EE969 pKa = 4.25YY970 pKa = 9.52TLQVSDD976 pKa = 5.14PDD978 pKa = 4.07DD979 pKa = 5.52ANDD982 pKa = 4.29GSGALNFSLSNPPAGMNISNTGEE1005 pKa = 4.13ITWTPVQGDD1014 pKa = 4.05ANGSSQVYY1022 pKa = 10.22NFTASVKK1029 pKa = 10.87DD1030 pKa = 3.56GGEE1033 pKa = 4.33DD1034 pKa = 3.62GATTAVQAIQITVAIPDD1051 pKa = 3.69ADD1053 pKa = 3.86GDD1055 pKa = 4.28TVADD1059 pKa = 4.11YY1060 pKa = 11.22EE1061 pKa = 4.8DD1062 pKa = 4.01NCPAVANTDD1071 pKa = 3.53QANNDD1076 pKa = 3.21ADD1078 pKa = 4.67AEE1080 pKa = 4.4GDD1082 pKa = 3.89LCDD1085 pKa = 6.31DD1086 pKa = 5.43DD1087 pKa = 6.7DD1088 pKa = 6.98DD1089 pKa = 6.01NDD1091 pKa = 3.89GMPDD1095 pKa = 3.72DD1096 pKa = 5.28FEE1098 pKa = 5.53VDD1100 pKa = 3.31NDD1102 pKa = 4.08FDD1104 pKa = 4.95PLDD1107 pKa = 4.17EE1108 pKa = 5.51SDD1110 pKa = 3.84AASDD1114 pKa = 3.7RR1115 pKa = 11.84DD1116 pKa = 3.44GDD1118 pKa = 4.38GISNLDD1124 pKa = 3.53EE1125 pKa = 4.31YY1126 pKa = 11.64LNNTDD1131 pKa = 4.04PSTDD1135 pKa = 2.98SVAPVITLAPEE1146 pKa = 4.0EE1147 pKa = 4.18VSRR1150 pKa = 11.84NAKK1153 pKa = 10.08GLLTPVEE1160 pKa = 4.57HH1161 pKa = 6.53SASAEE1166 pKa = 3.93DD1167 pKa = 5.38AIDD1170 pKa = 4.24GPVAVTADD1178 pKa = 3.22KK1179 pKa = 10.47TGPFASGHH1187 pKa = 6.14HH1188 pKa = 5.26VVTWQASDD1196 pKa = 3.22TSKK1199 pKa = 11.34NLAQSDD1205 pKa = 4.33QIIDD1209 pKa = 3.81IYY1211 pKa = 10.36PRR1213 pKa = 11.84VYY1215 pKa = 10.46VSPSQTVGEE1224 pKa = 4.31GQVVNVPIHH1233 pKa = 5.03ITGNAVSYY1241 pKa = 9.86PVTVYY1246 pKa = 10.58YY1247 pKa = 10.77SIDD1250 pKa = 3.55GATDD1254 pKa = 3.3SSDD1257 pKa = 3.32HH1258 pKa = 6.95DD1259 pKa = 4.35LVAGQIDD1266 pKa = 4.07ITSGHH1271 pKa = 5.2VSEE1274 pKa = 5.16LTFNTLADD1282 pKa = 3.84TLNEE1286 pKa = 4.02GSEE1289 pKa = 4.29SLNIRR1294 pKa = 11.84LTAADD1299 pKa = 4.13GASLSSAVNHH1309 pKa = 6.37QITITDD1315 pKa = 4.2LPAPPRR1321 pKa = 11.84INLLAAQDD1329 pKa = 3.71GHH1331 pKa = 5.06STRR1334 pKa = 11.84TIYY1337 pKa = 10.83LDD1339 pKa = 2.94QGLVHH1344 pKa = 6.63FSANASDD1351 pKa = 4.33PNGDD1355 pKa = 4.0TLSHH1359 pKa = 6.46TWTASISGINTISGGGSNDD1378 pKa = 3.52DD1379 pKa = 3.25HH1380 pKa = 8.15WEE1382 pKa = 3.82IDD1384 pKa = 3.65PQNLVAGQSINIRR1397 pKa = 11.84LSTHH1401 pKa = 6.85DD1402 pKa = 3.58GRR1404 pKa = 11.84HH1405 pKa = 4.89TAVQSLSLGIKK1416 pKa = 10.29DD1417 pKa = 4.25SAPLLDD1423 pKa = 4.57AFEE1426 pKa = 5.91DD1427 pKa = 3.93SDD1429 pKa = 5.18GDD1431 pKa = 4.42GIDD1434 pKa = 4.53DD1435 pKa = 4.65AAEE1438 pKa = 4.15GWGDD1442 pKa = 3.62SDD1444 pKa = 4.61GDD1446 pKa = 4.16GIANYY1451 pKa = 10.41LDD1453 pKa = 3.73STPLSSALQTSTGDD1467 pKa = 3.69FEE1469 pKa = 5.32TSLQQAIQTDD1479 pKa = 3.61PGLTLKK1485 pKa = 10.83LGSHH1489 pKa = 7.13ASAQGASKK1497 pKa = 10.94ASIAANSLLQTGNEE1511 pKa = 4.24DD1512 pKa = 3.45YY1513 pKa = 11.26QLIGQAFDD1521 pKa = 4.21FEE1523 pKa = 4.53IHH1525 pKa = 6.73GLDD1528 pKa = 3.28TSLTQAHH1535 pKa = 5.5VVIPLAVRR1543 pKa = 11.84IPSDD1547 pKa = 3.41DD1548 pKa = 3.46AVVRR1552 pKa = 11.84KK1553 pKa = 7.31YY1554 pKa = 10.56HH1555 pKa = 6.88PEE1557 pKa = 3.97HH1558 pKa = 6.47GWRR1561 pKa = 11.84AFNSSEE1567 pKa = 3.57GDD1569 pKa = 3.67TIHH1572 pKa = 7.23SLRR1575 pKa = 11.84SPDD1578 pKa = 3.54GTCPEE1583 pKa = 4.97PGSEE1587 pKa = 4.05QYY1589 pKa = 10.47QEE1591 pKa = 4.0GLAIFANCLQLTLTDD1606 pKa = 4.57GGPNDD1611 pKa = 3.71TDD1613 pKa = 3.7GVRR1616 pKa = 11.84NGIIVDD1622 pKa = 3.99PSAIAVPKK1630 pKa = 10.33QSNSSNDD1637 pKa = 3.21SSNEE1641 pKa = 3.79SAPRR1645 pKa = 11.84AGGSSGLSPLFFLWLLLISFAAARR1669 pKa = 11.84RR1670 pKa = 11.84RR1671 pKa = 11.84KK1672 pKa = 10.02ASIQITPSHH1681 pKa = 6.34

Molecular weight: 175.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5JT67|B5JT67_9GAMM General secretion pathway protein F OS=gamma proteobacterium HTCC5015 OX=391615 GN=GP5015_2504 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.2RR14 pKa = 11.84THH16 pKa = 6.01GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.28GRR39 pKa = 11.84HH40 pKa = 5.14SLSAA44 pKa = 3.67

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2449

0

2449

755730

30

2515

308.6

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.811 ± 0.065

0.971 ± 0.018

5.958 ± 0.041

6.696 ± 0.054

3.687 ± 0.032

7.434 ± 0.051

2.426 ± 0.03

5.247 ± 0.04

4.215 ± 0.048

10.33 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.024

3.367 ± 0.034

4.3 ± 0.028

4.618 ± 0.04

6.053 ± 0.05

6.297 ± 0.043

4.866 ± 0.041

7.044 ± 0.045

1.354 ± 0.023

2.832 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski