Enterobacteria phage Hgal1

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Leviviricetes; Norzivirales; Fiersviridae; Hagavirus; Hagavirus psychrophilum

Average proteome isoelectric point is 8.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6XDB9|I6XDB9_9VIRU Putative maturation/attachment protein OS=Enterobacteria phage Hgal1 OX=1206300 PE=4 SV=1
MM1 pKa = 7.6PQLQNLVLKK10 pKa = 10.77DD11 pKa = 3.51RR12 pKa = 11.84AATPKK17 pKa = 10.32SHH19 pKa = 6.75TFTPRR24 pKa = 11.84NVEE27 pKa = 4.12QNVGTVVEE35 pKa = 4.6TTGVPVGEE43 pKa = 4.2PRR45 pKa = 11.84FSISLRR51 pKa = 11.84QTADD55 pKa = 2.63NYY57 pKa = 10.46KK58 pKa = 10.53AEE60 pKa = 4.47LRR62 pKa = 11.84LMVPVVQDD70 pKa = 3.17QIINNVSSPVAVRR83 pKa = 11.84QAIASATFTFAKK95 pKa = 9.7TSTEE99 pKa = 3.75AEE101 pKa = 4.08RR102 pKa = 11.84NDD104 pKa = 3.51IVGMFADD111 pKa = 5.21ALATDD116 pKa = 3.6KK117 pKa = 11.08TLVNDD122 pKa = 4.16CVVKK126 pKa = 10.9LGGIYY131 pKa = 10.56GG132 pKa = 3.65

Molecular weight: 14.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6X3K8|I6X3K8_9VIRU Coat protein OS=Enterobacteria phage Hgal1 OX=1206300 PE=4 SV=1
MM1 pKa = 7.7ANPRR5 pKa = 11.84RR6 pKa = 11.84EE7 pKa = 4.08TLSVHH12 pKa = 6.26RR13 pKa = 11.84FIVTKK18 pKa = 10.51SQAQFSIRR26 pKa = 11.84IIRR29 pKa = 11.84CIRR32 pKa = 11.84NLLAIAGMLFLLSPIYY48 pKa = 10.29LDD50 pKa = 4.55KK51 pKa = 11.02FIQIYY56 pKa = 10.22LNAYY60 pKa = 7.19VVPMTT65 pKa = 4.57

Molecular weight: 7.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1127

65

523

281.8

31.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.897 ± 0.439

1.154 ± 0.339

4.969 ± 0.631

4.17 ± 0.361

4.614 ± 0.601

6.122 ± 0.592

1.508 ± 0.32

5.679 ± 0.961

5.59 ± 0.605

9.406 ± 0.471

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.686 ± 0.383

5.235 ± 0.487

4.614 ± 0.214

3.549 ± 0.329

6.921 ± 0.363

8.429 ± 0.887

6.477 ± 1.384

7.276 ± 0.907

1.863 ± 0.616

2.839 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski