Xanthobacter tagetidis
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4442 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3L7A1J7|A0A3L7A1J7_9RHIZ Urease subunit gamma OS=Xanthobacter tagetidis OX=60216 GN=D9R14_19810 PE=3 SV=1
TT1 pKa = 6.13 TAEE4 pKa = 4.26 DD5 pKa = 4.01 TPLVIAAADD14 pKa = 4.02 LLANDD19 pKa = 4.79 ADD21 pKa = 4.04 IDD23 pKa = 4.24 GDD25 pKa = 4.12 TLSVTGVGGAVGGTVALDD43 pKa = 3.73 DD44 pKa = 4.5 GEE46 pKa = 4.52 ITFTPDD52 pKa = 3.01 ADD54 pKa = 4.24 FNGDD58 pKa = 3.07 ASFTYY63 pKa = 9.55 TVSDD67 pKa = 3.78 GKK69 pKa = 11.16 GGTDD73 pKa = 2.94 TATVNVTVTPVNDD86 pKa = 3.85 APVAAADD93 pKa = 3.79 TATTAEE99 pKa = 4.31 DD100 pKa = 4.04 TPLVIAAADD109 pKa = 4.02 LLANDD114 pKa = 4.79 ADD116 pKa = 4.04 IDD118 pKa = 4.24 GDD120 pKa = 4.12 TLSVTGVGGAVGGTVALDD138 pKa = 3.83 DD139 pKa = 4.66 GEE141 pKa = 4.76 VTFTPDD147 pKa = 3.42 ANFNGDD153 pKa = 3.04 ASFTYY158 pKa = 9.55 TVSDD162 pKa = 3.78 GKK164 pKa = 11.16 GGTDD168 pKa = 2.94 TATVNVTVTPVNDD181 pKa = 3.85 APVAAADD188 pKa = 3.79 TATTAEE194 pKa = 4.31 DD195 pKa = 4.04 TPLVIAAADD204 pKa = 4.02 LLANDD209 pKa = 4.79 ADD211 pKa = 4.04 IDD213 pKa = 4.24 GDD215 pKa = 4.12 TLSVTGVGGAVGGTVALDD233 pKa = 3.83 DD234 pKa = 4.66 GEE236 pKa = 4.76 VTFTPDD242 pKa = 3.42 ANFNGDD248 pKa = 3.04 ASFTYY253 pKa = 9.55 TVSDD257 pKa = 3.78 GKK259 pKa = 11.16 GGTDD263 pKa = 2.94 TATVNVTVTPVNDD276 pKa = 3.74 APIATADD283 pKa = 3.57 TATTAEE289 pKa = 4.31 DD290 pKa = 4.14 TPLVIAVADD299 pKa = 4.29 LLANDD304 pKa = 4.6 SDD306 pKa = 5.16 LDD308 pKa = 4.06 GDD310 pKa = 4.22 TLSVTGVGGAVGGTVALDD328 pKa = 3.73 DD329 pKa = 4.5 GEE331 pKa = 4.52 ITFTPDD337 pKa = 3.01 ADD339 pKa = 4.24 FNGDD343 pKa = 3.01 ASFSYY348 pKa = 9.9 TVSDD352 pKa = 3.88 GKK354 pKa = 11.18 GGTDD358 pKa = 3.11 TATVGVTVTPVNDD371 pKa = 3.73 APVVASGATATFAEE385 pKa = 4.62 NGTGAVYY392 pKa = 10.33 QIAASDD398 pKa = 4.07 AEE400 pKa = 4.67 DD401 pKa = 5.25 DD402 pKa = 4.09 GLTYY406 pKa = 11.06 ALSGADD412 pKa = 3.29 ADD414 pKa = 4.86 LFTVDD419 pKa = 4.79 ADD421 pKa = 3.69 GKK423 pKa = 9.95 VAFKK427 pKa = 10.52 AAPDD431 pKa = 3.63 VEE433 pKa = 4.77 APADD437 pKa = 3.64 AGGDD441 pKa = 3.45 NVYY444 pKa = 11.09 DD445 pKa = 3.74 LVVEE449 pKa = 4.43 VSDD452 pKa = 4.56 GTDD455 pKa = 3.16 TTSKK459 pKa = 10.78 AVAITVTDD467 pKa = 3.61 VAEE470 pKa = 4.31 NSAPRR475 pKa = 11.84 LVSAPVLGGITAEE488 pKa = 4.02 AAAVGNGFSSSARR501 pKa = 11.84 FSPNGTKK508 pKa = 10.34 VLFQSTASNLVEE520 pKa = 4.24 GDD522 pKa = 3.52 ANGTEE527 pKa = 4.31 DD528 pKa = 4.81 VFVKK532 pKa = 10.6 DD533 pKa = 4.38 LEE535 pKa = 4.63 TGAVTLVSSSSSGTPGNGWSNGHH558 pKa = 5.75 QFSADD563 pKa = 3.1 GTKK566 pKa = 10.55 VLFYY570 pKa = 10.68 SYY572 pKa = 11.27 ASNLVAGDD580 pKa = 3.5 INGAADD586 pKa = 3.86 VFVKK590 pKa = 10.61 DD591 pKa = 4.39 LEE593 pKa = 4.63 TGAVTLVSSSSSGTPGNGEE612 pKa = 3.66 SSGYY616 pKa = 9.64 QFSADD621 pKa = 3.2 GTKK624 pKa = 10.55 VLFYY628 pKa = 10.83 SYY630 pKa = 11.43 ANNLVAGDD638 pKa = 3.84 INGVADD644 pKa = 3.75 VFVKK648 pKa = 10.69 NLEE651 pKa = 4.25 TGAVTLVSSSSSGTPGNGTSSGYY674 pKa = 9.85 QFSADD679 pKa = 3.2 GTKK682 pKa = 10.55 VLFYY686 pKa = 10.83 SYY688 pKa = 11.43 ANNLVAGDD696 pKa = 3.84 INGVADD702 pKa = 3.75 VFVKK706 pKa = 10.69 NLEE709 pKa = 4.25 TGAVTLVSSSSSGTPGNGTSYY730 pKa = 10.69 GHH732 pKa = 6.03 QFSADD737 pKa = 3.18 GTKK740 pKa = 10.55 VLFYY744 pKa = 10.83 SYY746 pKa = 11.43 ANNLVAGDD754 pKa = 3.84 INGVADD760 pKa = 3.75 VFVKK764 pKa = 10.69 NLEE767 pKa = 4.25 TGAVTLVSSSSSGTPGNGTSYY788 pKa = 10.7 GYY790 pKa = 10.37 QYY792 pKa = 11.09 SADD795 pKa = 3.58 GTKK798 pKa = 10.58 VLFQSDD804 pKa = 3.69 ASNLVAGDD812 pKa = 3.5 INGAADD818 pKa = 3.69 IFVKK822 pKa = 10.61 DD823 pKa = 4.06 LEE825 pKa = 4.72 TGVVTLVSSSSSGTPGNQTSYY846 pKa = 10.27 SHH848 pKa = 6.19 QFSADD853 pKa = 3.09 GTKK856 pKa = 10.47 VLFYY860 pKa = 10.89 SSASNLVAGDD870 pKa = 3.5 INGAADD876 pKa = 3.86 VFVKK880 pKa = 10.53 DD881 pKa = 4.33 LEE883 pKa = 4.74 SGAVTLVSSSSSGTPGNGTSYY904 pKa = 10.69 GHH906 pKa = 6.21 QFSADD911 pKa = 3.35 CAKK914 pKa = 10.82 VLFRR918 pKa = 11.84 SYY920 pKa = 11.39 ASNLVAGDD928 pKa = 3.6 TNGTYY933 pKa = 10.75 DD934 pKa = 4.52 VFMKK938 pKa = 10.72 DD939 pKa = 3.12 LATGEE944 pKa = 4.33 VTLLSARR951 pKa = 11.84 SLAATPLAKK960 pKa = 10.31 SGAFTFDD967 pKa = 3.91 DD968 pKa = 4.07 ADD970 pKa = 4.01 GGDD973 pKa = 3.38 THH975 pKa = 6.5 TASVLPAAGTLGTLQADD992 pKa = 3.52 IVVVADD998 pKa = 3.94 GPDD1001 pKa = 3.29 RR1002 pKa = 11.84 VEE1004 pKa = 3.59 WSYY1007 pKa = 11.72 SANRR1011 pKa = 11.84 TLFAALAEE1019 pKa = 4.48 GEE1021 pKa = 4.69 SAFEE1025 pKa = 4.2 TFTVTVDD1032 pKa = 3.64 DD1033 pKa = 3.96 GHH1035 pKa = 6.68 GGSFSHH1041 pKa = 7.17 DD1042 pKa = 3.24 VVIEE1046 pKa = 4.03 VQGVNDD1052 pKa = 3.68 VPVAVADD1059 pKa = 3.98 SLSTAEE1065 pKa = 4.48 DD1066 pKa = 4.0 TPLVIAAADD1075 pKa = 3.93 LLANDD1080 pKa = 4.58 SDD1082 pKa = 4.26 VDD1084 pKa = 3.91 GDD1086 pKa = 4.03 TLTLVSVGGAVGGSVSLDD1104 pKa = 2.86 AGQITFTPYY1113 pKa = 10.44 TDD1115 pKa = 3.93 FNGAASFAYY1124 pKa = 9.48 MIADD1128 pKa = 3.85 GKK1130 pKa = 11.29 GGFDD1134 pKa = 3.35 SATVAVTFTPVNDD1147 pKa = 3.67 APVVGGQTSGAVVEE1161 pKa = 4.93 DD1162 pKa = 3.94 RR1163 pKa = 11.84 TTSVSGALTIADD1175 pKa = 4.34 LDD1177 pKa = 4.05 AGEE1180 pKa = 5.02 SAFQAQSGIAGTYY1193 pKa = 10.47 GMLNINEE1200 pKa = 4.2 QGQWTYY1206 pKa = 11.02 FLSNASQAVQMLRR1219 pKa = 11.84 GDD1221 pKa = 4.46 GYY1223 pKa = 9.43 PWSDD1227 pKa = 3.05 NFTVLTADD1235 pKa = 3.57 GTEE1238 pKa = 3.91 QTISISVLGADD1249 pKa = 3.9 DD1250 pKa = 4.3 TVTATAPTTYY1260 pKa = 9.48 TASTFTTLPVYY1271 pKa = 10.85 ASGITVDD1278 pKa = 3.9 SEE1280 pKa = 4.05 TGYY1283 pKa = 11.4 VYY1285 pKa = 10.66 VAEE1288 pKa = 4.48 KK1289 pKa = 10.98 APVSKK1294 pKa = 10.41 LYY1296 pKa = 10.56 KK1297 pKa = 9.2 VAPNGAVTQIASDD1310 pKa = 3.57 FTGYY1314 pKa = 9.19 TSGMGGYY1321 pKa = 10.3 CYY1323 pKa = 10.05 GTGDD1327 pKa = 3.27 IEE1329 pKa = 4.75 YY1330 pKa = 9.73 YY1331 pKa = 10.25 DD1332 pKa = 3.35 GKK1334 pKa = 11.28 VYY1336 pKa = 10.99 VVTDD1340 pKa = 3.56 AGKK1343 pKa = 10.47 LVGIDD1348 pKa = 3.29 VATGARR1354 pKa = 11.84 SVLFTLPGGAGGPVLDD1370 pKa = 4.95 AGIAITPDD1378 pKa = 3.14 GKK1380 pKa = 11.17 VLMSNGQSAPAGVWSYY1396 pKa = 11.36 DD1397 pKa = 3.09 IEE1399 pKa = 4.46 TGASGFVASTPGATFEE1415 pKa = 4.72 VDD1417 pKa = 5.02 FDD1419 pKa = 4.04 PATNKK1424 pKa = 9.23 TVVGWPQNAVLTVNYY1439 pKa = 10.59 AEE1441 pKa = 5.6 DD1442 pKa = 3.29 STGDD1446 pKa = 3.45 YY1447 pKa = 10.4 RR1448 pKa = 11.84 GVGTPFYY1455 pKa = 10.99 NFALSPGGDD1464 pKa = 3.51 EE1465 pKa = 4.06 IYY1467 pKa = 10.78 LRR1469 pKa = 11.84 SGSSLVQVNLGTGSSALVGSGLFNDD1494 pKa = 4.36 SSQDD1498 pKa = 3.62 LEE1500 pKa = 4.57 FGPSSSGGGYY1510 pKa = 9.71 SLYY1513 pKa = 10.47 VVNVSTILEE1522 pKa = 4.32 FKK1524 pKa = 11.03 GFTTEE1529 pKa = 4.01 WAQLPQDD1536 pKa = 4.52 PDD1538 pKa = 4.77 PIVLDD1543 pKa = 3.83 LDD1545 pKa = 3.86 GDD1547 pKa = 4.02 GTYY1550 pKa = 10.55 FSAPGTGVSFDD1561 pKa = 4.18 LDD1563 pKa = 3.5 GDD1565 pKa = 4.05 GTAEE1569 pKa = 4.05 LLGWASPADD1578 pKa = 3.63 GVLVMDD1584 pKa = 5.51 LDD1586 pKa = 4.22 GSGAIEE1592 pKa = 4.46 NGTEE1596 pKa = 4.19 VVSEE1600 pKa = 4.05 AFKK1603 pKa = 11.3 GFGFGSSVDD1612 pKa = 3.56 ALRR1615 pKa = 11.84 SLDD1618 pKa = 3.75 TNLDD1622 pKa = 3.44 GMLDD1626 pKa = 3.5 AGDD1629 pKa = 3.96 AAFADD1634 pKa = 3.72 LRR1636 pKa = 11.84 LWRR1639 pKa = 11.84 DD1640 pKa = 3.22 GNGDD1644 pKa = 3.55 GGSQGDD1650 pKa = 4.11 EE1651 pKa = 4.19 LLTLANLGITAISVEE1666 pKa = 3.95 AQARR1670 pKa = 11.84 DD1671 pKa = 3.65 EE1672 pKa = 4.5 TVDD1675 pKa = 3.34 GHH1677 pKa = 6.36 HH1678 pKa = 6.94 VYY1680 pKa = 10.6 AAGSFSYY1687 pKa = 11.05 ASGATGSYY1695 pKa = 9.55 VGARR1699 pKa = 11.84 FAPASAGATDD1709 pKa = 4.97 LEE1711 pKa = 4.9 SGDD1714 pKa = 3.75 EE1715 pKa = 4.37 TEE1717 pKa = 5.45 VIAEE1721 pKa = 4.52 LFSAEE1726 pKa = 4.14 DD1727 pKa = 3.89 PEE1729 pKa = 4.42 MEE1731 pKa = 4.51 AALASALGASAPSGLRR1747 pKa = 11.84 STRR1750 pKa = 11.84 ADD1752 pKa = 3.36 ALLALQDD1759 pKa = 5.82 FFDD1762 pKa = 4.84 DD1763 pKa = 3.63 WQSFPGTSPAPADD1776 pKa = 3.38 LRR1778 pKa = 11.84 AAIGQWIGEE1787 pKa = 4.43 VAFGDD1792 pKa = 3.76 HH1793 pKa = 6.73 SPTVDD1798 pKa = 3.83 DD1799 pKa = 4.03 LLHH1802 pKa = 7.05 RR1803 pKa = 11.84 FGHH1806 pKa = 6.8 DD1807 pKa = 3.09 PAFGNLLQGAHH1818 pKa = 6.43 ASVNDD1823 pKa = 3.58 MFIVV1827 pKa = 3.58
Molecular weight: 183.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.49
IPC_protein 3.554
Toseland 3.312
ProMoST 3.732
Dawson 3.567
Bjellqvist 3.719
Wikipedia 3.528
Rodwell 3.376
Grimsley 3.21
Solomon 3.567
Lehninger 3.528
Nozaki 3.668
DTASelect 3.986
Thurlkill 3.363
EMBOSS 3.528
Sillero 3.681
Patrickios 1.621
IPC_peptide 3.554
IPC2_peptide 3.656
IPC2.peptide.svr19 3.689
Protein with the highest isoelectric point:
>tr|A0A3L7ANG0|A0A3L7ANG0_9RHIZ Cobalt-precorrin-5B C(1)-methyltransferase OS=Xanthobacter tagetidis OX=60216 GN=cbiD PE=3 SV=1
MM1 pKa = 7.48 PRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 SRR7 pKa = 11.84 SRR9 pKa = 11.84 RR10 pKa = 11.84 SPWPAPRR17 pKa = 11.84 RR18 pKa = 11.84 SPTRR22 pKa = 11.84 PRR24 pKa = 11.84 PTSARR29 pKa = 11.84 PASMRR34 pKa = 11.84 RR35 pKa = 11.84 PRR37 pKa = 11.84 PFTRR41 pKa = 11.84 PPHH44 pKa = 5.94 LASPPRR50 pKa = 11.84 RR51 pKa = 11.84 TSAASSPTRR60 pKa = 11.84 CAGWW64 pKa = 3.2
Molecular weight: 7.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.501
IPC2_protein 11.125
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.378
Rodwell 12.398
Grimsley 12.939
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.135
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.181
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4442
0
4442
1423151
29
3175
320.4
34.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.58 ± 0.064
0.862 ± 0.012
5.376 ± 0.031
5.413 ± 0.033
3.694 ± 0.025
9.091 ± 0.06
1.919 ± 0.019
4.565 ± 0.029
2.944 ± 0.029
10.358 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.373 ± 0.016
2.089 ± 0.022
5.797 ± 0.038
2.707 ± 0.019
7.327 ± 0.043
4.79 ± 0.03
4.954 ± 0.029
7.919 ± 0.028
1.203 ± 0.014
2.039 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here