Streptomyces phage Madamato

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rimavirus; unclassified Rimavirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CTE6|A0A411CTE6_9CAUD Uncharacterized protein OS=Streptomyces phage Madamato OX=2510517 GN=4 PE=4 SV=1
MM1 pKa = 7.24TQLTWDD7 pKa = 3.45KK8 pKa = 11.14SGEE11 pKa = 4.07RR12 pKa = 11.84YY13 pKa = 9.81YY14 pKa = 10.45EE15 pKa = 3.85TGVDD19 pKa = 3.65HH20 pKa = 6.89GVLYY24 pKa = 10.48IPNSQGDD31 pKa = 4.11YY32 pKa = 9.8VSGYY36 pKa = 8.68SWNGLTTVTEE46 pKa = 4.44SPSGAEE52 pKa = 4.33SNPQYY57 pKa = 11.32ADD59 pKa = 3.31NIKK62 pKa = 10.1YY63 pKa = 10.68LNLISAEE70 pKa = 4.08EE71 pKa = 3.89FGGTIEE77 pKa = 4.71AFTYY81 pKa = 10.2PDD83 pKa = 3.98EE84 pKa = 5.39FGQCDD89 pKa = 3.72GTAAPTPGVLLGQQSRR105 pKa = 11.84KK106 pKa = 7.81TFGLSYY112 pKa = 9.42RR113 pKa = 11.84TKK115 pKa = 10.7LGNDD119 pKa = 3.95LDD121 pKa = 4.55GQDD124 pKa = 4.02HH125 pKa = 7.09GYY127 pKa = 10.58KK128 pKa = 10.3LHH130 pKa = 6.48LVYY133 pKa = 10.45GALAAPSEE141 pKa = 4.28KK142 pKa = 10.51AYY144 pKa = 9.79ATVNDD149 pKa = 4.08SPEE152 pKa = 4.78AITFSWEE159 pKa = 3.78FTTTPVEE166 pKa = 4.21VGQIAGVDD174 pKa = 3.7YY175 pKa = 10.95KK176 pKa = 10.33PTATLTIDD184 pKa = 3.51STKK187 pKa = 10.49VDD189 pKa = 3.76AGALDD194 pKa = 3.67TLEE197 pKa = 4.08EE198 pKa = 4.13FLYY201 pKa = 9.09GTEE204 pKa = 4.23GTDD207 pKa = 3.99PSLPDD212 pKa = 4.04PATVIAMFSGTVLEE226 pKa = 4.45ATPTEE231 pKa = 4.15PTFDD235 pKa = 4.12AGTDD239 pKa = 3.74TITIPAITGVEE250 pKa = 4.23YY251 pKa = 11.06YY252 pKa = 9.96IGGVLQPAGPVIISQDD268 pKa = 3.79TIVTARR274 pKa = 11.84PAKK277 pKa = 10.16GYY279 pKa = 10.19KK280 pKa = 10.01FPANVDD286 pKa = 3.58NDD288 pKa = 3.73WLIVYY293 pKa = 8.81SS294 pKa = 4.02

Molecular weight: 31.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CTI3|A0A411CTI3_9CAUD Uncharacterized protein OS=Streptomyces phage Madamato OX=2510517 GN=41 PE=4 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84YY3 pKa = 9.49YY4 pKa = 10.89GKK6 pKa = 9.64NWSVSNHH13 pKa = 6.32KK14 pKa = 10.0IGNGSKK20 pKa = 10.16QVGLAIWWNLARR32 pKa = 11.84VGFQINAGIRR42 pKa = 11.84EE43 pKa = 4.01IQIMRR48 pKa = 11.84EE49 pKa = 3.96RR50 pKa = 11.84KK51 pKa = 9.57LKK53 pKa = 10.63

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

17232

33

1829

202.7

22.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.036 ± 0.539

0.528 ± 0.109

6.041 ± 0.352

7.399 ± 0.426

3.557 ± 0.224

7.608 ± 0.466

1.822 ± 0.205

5.374 ± 0.181

6.888 ± 0.42

7.979 ± 0.444

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.143

3.975 ± 0.163

4.051 ± 0.342

3.308 ± 0.185

5.02 ± 0.431

6.378 ± 0.273

6.708 ± 0.305

6.9 ± 0.212

1.37 ± 0.128

3.418 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski