Streptococcus satellite phage Javan431
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZSV8|A0A4D5ZSV8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan431 OX=2558700 GN=JavanS431_0008 PE=4 SV=1
MM1 pKa = 7.61 NNTQVYY7 pKa = 8.59 QAQTTNHH14 pKa = 6.71 GIARR18 pKa = 11.84 ALGEE22 pKa = 4.87 EE23 pKa = 3.69 IRR25 pKa = 11.84 MITDD29 pKa = 3.35 VEE31 pKa = 4.1 EE32 pKa = 4.15 TNYY35 pKa = 10.66 NGHH38 pKa = 6.24 GEE40 pKa = 4.07 ITVEE44 pKa = 4.03 YY45 pKa = 8.99 TNDD48 pKa = 3.01 VALTKK53 pKa = 10.8 LFDD56 pKa = 5.08 RR57 pKa = 11.84 IKK59 pKa = 10.53 SLYY62 pKa = 9.62 EE63 pKa = 3.72 KK64 pKa = 10.85 GEE66 pKa = 4.17 SYY68 pKa = 7.74 EE69 pKa = 4.29 TKK71 pKa = 10.25 PFLHH75 pKa = 6.71 FKK77 pKa = 10.38 ILEE80 pKa = 4.7 DD81 pKa = 4.67 DD82 pKa = 4.84 LEE84 pKa = 4.48 TKK86 pKa = 10.51 PGVFTAKK93 pKa = 9.88 EE94 pKa = 3.88 VADD97 pKa = 4.06 FLGVV101 pKa = 3.18
Molecular weight: 11.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.741
IPC2_protein 4.787
IPC_protein 4.647
Toseland 4.533
ProMoST 4.749
Dawson 4.596
Bjellqvist 4.736
Wikipedia 4.444
Rodwell 4.507
Grimsley 4.444
Solomon 4.584
Lehninger 4.546
Nozaki 4.711
DTASelect 4.813
Thurlkill 4.52
EMBOSS 4.469
Sillero 4.774
Patrickios 3.859
IPC_peptide 4.596
IPC2_peptide 4.774
IPC2.peptide.svr19 4.707
Protein with the highest isoelectric point:
>tr|A0A4D5ZN39|A0A4D5ZN39_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan431 OX=2558700 GN=JavanS431_0007 PE=4 SV=1
MM1 pKa = 7.37 TNYY4 pKa = 10.27 DD5 pKa = 3.45 RR6 pKa = 11.84 AKK8 pKa = 10.18 RR9 pKa = 11.84 FYY11 pKa = 11.32 SRR13 pKa = 11.84 ATYY16 pKa = 10.67 ALSDD20 pKa = 3.97 FLHH23 pKa = 6.24 GRR25 pKa = 11.84 IDD27 pKa = 3.42 EE28 pKa = 4.27 QEE30 pKa = 4.16 FRR32 pKa = 11.84 KK33 pKa = 9.32 QWQRR37 pKa = 11.84 LADD40 pKa = 3.62 KK41 pKa = 10.58 AVNEE45 pKa = 4.21 MKK47 pKa = 10.81 KK48 pKa = 10.42 NN49 pKa = 3.49
Molecular weight: 6.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.069
IPC2_protein 9.224
IPC_protein 9.326
Toseland 9.882
ProMoST 9.589
Dawson 10.101
Bjellqvist 9.765
Wikipedia 10.277
Rodwell 10.452
Grimsley 10.189
Solomon 10.16
Lehninger 10.131
Nozaki 9.838
DTASelect 9.78
Thurlkill 9.94
EMBOSS 10.292
Sillero 10.014
Patrickios 10.16
IPC_peptide 10.16
IPC2_peptide 8.317
IPC2.peptide.svr19 8.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
2702
48
484
158.9
18.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.477 ± 0.581
0.444 ± 0.126
5.922 ± 0.523
9.03 ± 0.946
5.107 ± 0.514
4.774 ± 0.516
1.702 ± 0.155
6.44 ± 0.26
10.215 ± 0.491
10.585 ± 0.792
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.11 ± 0.229
5.403 ± 0.381
2.48 ± 0.287
4.293 ± 0.476
4.737 ± 0.571
4.811 ± 0.383
5.699 ± 0.59
5.292 ± 0.343
0.888 ± 0.128
4.589 ± 0.435
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here