Capybara microvirus Cap1_SP_230

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W5H5|A0A4P8W5H5_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_230 OX=2585416 PE=4 SV=1
MM1 pKa = 7.9DD2 pKa = 4.59FSTRR6 pKa = 11.84YY7 pKa = 9.64RR8 pKa = 11.84PFKK11 pKa = 10.78AKK13 pKa = 10.2PCDD16 pKa = 3.61FFGDD20 pKa = 3.46EE21 pKa = 4.77RR22 pKa = 11.84ILVDD26 pKa = 3.73SSHH29 pKa = 7.16RR30 pKa = 11.84DD31 pKa = 3.23QVDD34 pKa = 2.77INNILKK40 pKa = 10.25RR41 pKa = 11.84YY42 pKa = 7.82RR43 pKa = 11.84QTGVLPPSLAQAVFADD59 pKa = 3.81TSIFTDD65 pKa = 3.61YY66 pKa = 10.71KK67 pKa = 10.88AALATIRR74 pKa = 11.84EE75 pKa = 4.38AEE77 pKa = 4.75DD78 pKa = 3.14IFASMPAEE86 pKa = 3.61IRR88 pKa = 11.84EE89 pKa = 4.32SCGYY93 pKa = 9.99EE94 pKa = 3.91PSEE97 pKa = 4.18LLAFMSRR104 pKa = 11.84PDD106 pKa = 4.2SKK108 pKa = 11.15PLIQKK113 pKa = 9.25YY114 pKa = 9.96FGGGVLPNQVAEE126 pKa = 4.49ANPAGISSPSPTKK139 pKa = 10.67KK140 pKa = 9.94EE141 pKa = 3.79

Molecular weight: 15.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W878|A0A4P8W878_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_230 OX=2585416 PE=4 SV=1
MM1 pKa = 7.44ICEE4 pKa = 4.31SPLQAFCLDD13 pKa = 3.83PSAQKK18 pKa = 10.62VCGIVDD24 pKa = 4.05FSKK27 pKa = 10.95LVDD30 pKa = 3.62GLTPRR35 pKa = 11.84IIFNSWLVNGAFPKK49 pKa = 10.45LCSLMEE55 pKa = 3.9QRR57 pKa = 11.84LCRR60 pKa = 11.84PVMLPCGKK68 pKa = 10.05CLACRR73 pKa = 11.84NRR75 pKa = 11.84HH76 pKa = 6.16LIDD79 pKa = 3.12VSLRR83 pKa = 11.84MQHH86 pKa = 6.08EE87 pKa = 5.55LITTVQPSTFLTLTYY102 pKa = 10.95NEE104 pKa = 4.08AHH106 pKa = 6.42SCNTLDD112 pKa = 4.11YY113 pKa = 11.56GDD115 pKa = 3.72IQSFLKK121 pKa = 10.54RR122 pKa = 11.84LRR124 pKa = 11.84TKK126 pKa = 10.44ALSPFRR132 pKa = 11.84FSCCGEE138 pKa = 3.71YY139 pKa = 10.95GKK141 pKa = 10.4LHH143 pKa = 6.24NRR145 pKa = 11.84PHH147 pKa = 5.49WHH149 pKa = 6.45FILYY153 pKa = 9.95GFYY156 pKa = 10.21PSDD159 pKa = 5.56GILQSQTRR167 pKa = 11.84GGNYY171 pKa = 9.29YY172 pKa = 10.75VSDD175 pKa = 4.8TISKK179 pKa = 8.12IWPFGFHH186 pKa = 6.73LFAPASAGACSYY198 pKa = 9.67LAKK201 pKa = 10.78YY202 pKa = 9.67LVKK205 pKa = 10.62QEE207 pKa = 4.66DD208 pKa = 4.82GIFKK212 pKa = 10.3QSLRR216 pKa = 11.84PGVGFEE222 pKa = 4.26YY223 pKa = 9.93YY224 pKa = 9.93RR225 pKa = 11.84KK226 pKa = 9.22YY227 pKa = 10.47HH228 pKa = 5.54VEE230 pKa = 4.44FYY232 pKa = 11.29SRR234 pKa = 11.84GFCVMNGFKK243 pKa = 10.78LPIPDD248 pKa = 3.54YY249 pKa = 10.53YY250 pKa = 11.43DD251 pKa = 5.21RR252 pKa = 11.84ILEE255 pKa = 4.29RR256 pKa = 11.84EE257 pKa = 4.18DD258 pKa = 2.96KK259 pKa = 11.0KK260 pKa = 10.93MYY262 pKa = 10.83EE263 pKa = 4.23EE264 pKa = 4.37VCKK267 pKa = 10.65KK268 pKa = 10.3RR269 pKa = 11.84LPCYY273 pKa = 10.35NIYY276 pKa = 10.62DD277 pKa = 3.76LSEE280 pKa = 3.97FLDD283 pKa = 4.02FHH285 pKa = 7.56RR286 pKa = 11.84HH287 pKa = 5.14DD288 pKa = 4.73AYY290 pKa = 10.72HH291 pKa = 6.67GKK293 pKa = 9.8ARR295 pKa = 11.84CLKK298 pKa = 10.42ISTQPEE304 pKa = 4.11KK305 pKa = 11.11GVLL308 pKa = 3.49

Molecular weight: 35.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1283

141

553

320.8

36.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.638 ± 2.093

1.637 ± 1.06

5.456 ± 0.429

6.002 ± 1.048

6.08 ± 0.965

6.859 ± 0.475

2.338 ± 0.371

3.663 ± 0.718

5.612 ± 1.34

9.119 ± 0.594

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.338 ± 0.343

3.897 ± 0.44

5.066 ± 0.85

4.521 ± 0.622

5.3 ± 0.497

8.496 ± 0.903

4.91 ± 0.92

5.846 ± 0.975

1.091 ± 0.177

4.131 ± 0.594
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski