Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Leptothrix; Leptothrix cholodnii

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4343 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B1Y8I5|RL23_LEPCP 50S ribosomal protein L23 OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) OX=395495 GN=rplW PE=3 SV=1
MM1 pKa = 7.59CLICGWIYY9 pKa = 11.2DD10 pKa = 4.23EE11 pKa = 5.21AAGDD15 pKa = 4.24PDD17 pKa = 4.79HH18 pKa = 7.53DD19 pKa = 4.54LAPGTRR25 pKa = 11.84WADD28 pKa = 3.22VPMNWVCPEE37 pKa = 3.74CGARR41 pKa = 11.84KK42 pKa = 9.54EE43 pKa = 4.09DD44 pKa = 3.83FEE46 pKa = 4.5MVQVV50 pKa = 3.71

Molecular weight: 5.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1Y0L6|B1Y0L6_LEPCP Peptidase_S15 domain-containing protein OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) OX=395495 GN=Lcho_0722 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.87VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 10.51SRR25 pKa = 11.84GGRR28 pKa = 11.84SVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4343

0

4343

1495830

31

5613

344.4

37.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.544 ± 0.057

0.939 ± 0.013

5.53 ± 0.032

5.067 ± 0.032

3.197 ± 0.021

8.425 ± 0.033

2.378 ± 0.022

4.175 ± 0.025

2.711 ± 0.033

11.052 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.018

2.374 ± 0.024

5.366 ± 0.035

4.029 ± 0.022

7.391 ± 0.044

5.31 ± 0.033

5.101 ± 0.043

7.636 ± 0.031

1.49 ± 0.016

1.997 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski