Human immunodeficiency virus type 1 group M subtype D (isolate ELI) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 unknown group

Average proteome isoelectric point is 7.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P05956|VPR_HV1EL Protein Vpr OS=Human immunodeficiency virus type 1 group M subtype D (isolate ELI) OX=11689 GN=vpr PE=3 SV=1
MM1 pKa = 7.14QPLGIIAIAALVVAIILAIVVWTIVFIEE29 pKa = 3.8YY30 pKa = 10.32RR31 pKa = 11.84RR32 pKa = 11.84IKK34 pKa = 8.69KK35 pKa = 8.34QRR37 pKa = 11.84RR38 pKa = 11.84IDD40 pKa = 3.74CLLDD44 pKa = 4.71RR45 pKa = 11.84ITEE48 pKa = 4.09RR49 pKa = 11.84AEE51 pKa = 4.09DD52 pKa = 3.66SGNEE56 pKa = 4.05SEE58 pKa = 4.91GDD60 pKa = 3.42RR61 pKa = 11.84EE62 pKa = 4.18KK63 pKa = 11.08LSKK66 pKa = 10.24LVEE69 pKa = 4.43MGHH72 pKa = 6.07HH73 pKa = 6.53APWDD77 pKa = 3.51IDD79 pKa = 3.74DD80 pKa = 4.17LL81 pKa = 5.12

Molecular weight: 9.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P04604|NEF_HV1EL Protein Nef OS=Human immunodeficiency virus type 1 group M subtype D (isolate ELI) OX=11689 GN=nef PE=3 SV=3
MM1 pKa = 7.3EE2 pKa = 5.21NRR4 pKa = 11.84WQVMIVWQVDD14 pKa = 3.01RR15 pKa = 11.84MRR17 pKa = 11.84IKK19 pKa = 8.93TWKK22 pKa = 10.27SLVKK26 pKa = 9.92HH27 pKa = 5.93HH28 pKa = 6.62MYY30 pKa = 10.3VSKK33 pKa = 10.69KK34 pKa = 9.11ANRR37 pKa = 11.84WFYY40 pKa = 8.75RR41 pKa = 11.84HH42 pKa = 6.55HH43 pKa = 6.98YY44 pKa = 8.54EE45 pKa = 4.5SPHH48 pKa = 5.99PKK50 pKa = 9.39ISSEE54 pKa = 3.83VHH56 pKa = 5.47IPLGEE61 pKa = 3.87ARR63 pKa = 11.84LVIKK67 pKa = 7.97TYY69 pKa = 9.61WGLHH73 pKa = 4.19TGEE76 pKa = 4.9RR77 pKa = 11.84EE78 pKa = 3.63WHH80 pKa = 6.26LGQGVSIEE88 pKa = 3.9WRR90 pKa = 11.84KK91 pKa = 9.69RR92 pKa = 11.84RR93 pKa = 11.84YY94 pKa = 8.15STQVDD99 pKa = 3.64PGLADD104 pKa = 3.49QLIHH108 pKa = 6.69MYY110 pKa = 10.84YY111 pKa = 10.01FDD113 pKa = 5.16CFSEE117 pKa = 4.31SAIRR121 pKa = 11.84KK122 pKa = 9.37AILGDD127 pKa = 3.44IVSPRR132 pKa = 11.84CEE134 pKa = 3.92YY135 pKa = 10.43QAGHH139 pKa = 6.19NKK141 pKa = 9.66VGSLQYY147 pKa = 10.82LALTALIAPKK157 pKa = 9.93QIKK160 pKa = 9.61PPLPSVRR167 pKa = 11.84KK168 pKa = 8.46LTEE171 pKa = 4.09DD172 pKa = 2.93RR173 pKa = 11.84WNKK176 pKa = 8.03PQQTRR181 pKa = 11.84GHH183 pKa = 6.48RR184 pKa = 11.84GSHH187 pKa = 4.85TMNGHH192 pKa = 6.05

Molecular weight: 22.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3652

72

1435

365.2

41.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.38 ± 0.467

2.136 ± 0.455

3.779 ± 0.351

7.065 ± 0.473

2.3 ± 0.155

7.229 ± 0.261

2.245 ± 0.519

6.955 ± 0.632

6.982 ± 0.983

8.215 ± 0.564

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.136 ± 0.211

4.737 ± 0.699

5.75 ± 0.913

5.969 ± 0.397

6.38 ± 0.843

5.367 ± 0.562

5.531 ± 0.504

5.559 ± 0.429

2.683 ± 0.287

2.601 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski