Legionella tucsonensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2936 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W0ZVW2|A0A0W0ZVW2_9GAMM Uncharacterized protein OS=Legionella tucsonensis OX=40335 GN=Ltuc_0807 PE=4 SV=1
MM1 pKa = 7.74IFDD4 pKa = 4.38AALSGLQAATSNLDD18 pKa = 3.51VIGNNIANSSTVGFKK33 pKa = 10.62GSRR36 pKa = 11.84ANFGDD41 pKa = 3.58IYY43 pKa = 11.32AFGGYY48 pKa = 10.18GSFGAGSTSIGSGVMLTQVQQSFASGNLSSSTNSLDD84 pKa = 3.65LAVNGSGFFILNNPGGGAVYY104 pKa = 9.6TRR106 pKa = 11.84AGQFNLNNQNYY117 pKa = 6.59ITNANGQYY125 pKa = 9.31LTGLLSDD132 pKa = 4.04GKK134 pKa = 11.41GNITGTSGKK143 pKa = 9.95LQINTEE149 pKa = 4.26NITPQASTTVTTGVNLNSQSTPPAIDD175 pKa = 3.15WVGGAAPLSSSYY187 pKa = 11.57NNPTSLTIYY196 pKa = 10.51DD197 pKa = 3.93SLGNSHH203 pKa = 6.36VLSMYY208 pKa = 10.4FIKK211 pKa = 10.61ADD213 pKa = 3.32SAAAAGQPNASTPPGTEE230 pKa = 3.85NQWYY234 pKa = 9.58VAFQIDD240 pKa = 4.25NQNVPANAGTDD251 pKa = 3.11NTTNLFEE258 pKa = 5.22VNFNSDD264 pKa = 2.82GSFVGVASPGGAPIGDD280 pKa = 3.71NLIPLSLNLNNGANPLNLNIDD301 pKa = 4.53LSNSTQFGSPFGVQSTSSNGYY322 pKa = 4.73TTGSLASLSIDD333 pKa = 3.31DD334 pKa = 4.18TGLILGIYY342 pKa = 9.84TNGQSMAMGQIQLANFADD360 pKa = 4.04PTGLQNMGNVSWAEE374 pKa = 4.04TSASGQPLIGTAASGGFGAIKK395 pKa = 10.31SGMLEE400 pKa = 3.86QSNVDD405 pKa = 3.72LTSEE409 pKa = 4.03LVDD412 pKa = 5.38LISAQRR418 pKa = 11.84DD419 pKa = 3.74FQANAQSIRR428 pKa = 11.84AGDD431 pKa = 4.32TITQTIINMRR441 pKa = 3.79

Molecular weight: 45.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W0ZQJ4|A0A0W0ZQJ4_9GAMM Histidine ammonia lyase OS=Legionella tucsonensis OX=40335 GN=Ltuc_2647 PE=4 SV=1
MM1 pKa = 7.67AEE3 pKa = 3.9VKK5 pKa = 10.18QYY7 pKa = 11.26YY8 pKa = 7.84GTGRR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 10.28SSTARR19 pKa = 11.84VFLRR23 pKa = 11.84PGKK26 pKa = 10.95GEE28 pKa = 3.68IKK30 pKa = 10.85VNGRR34 pKa = 11.84TLQEE38 pKa = 4.03YY39 pKa = 9.14FCRR42 pKa = 11.84EE43 pKa = 4.03TSCMVVMQPLEE54 pKa = 4.23TVDD57 pKa = 4.21LVNKK61 pKa = 9.67FDD63 pKa = 3.85VYY65 pKa = 10.13VTVSGGGISGQAGAVRR81 pKa = 11.84LGIARR86 pKa = 11.84ALVAYY91 pKa = 10.12DD92 pKa = 3.55EE93 pKa = 4.77TGLAEE98 pKa = 4.51DD99 pKa = 5.2AEE101 pKa = 4.64PNPNSVRR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84LRR112 pKa = 11.84ARR114 pKa = 11.84GLLTRR119 pKa = 11.84DD120 pKa = 2.92SRR122 pKa = 11.84RR123 pKa = 11.84VEE125 pKa = 3.91RR126 pKa = 11.84KK127 pKa = 9.53KK128 pKa = 11.16VGLHH132 pKa = 5.33KK133 pKa = 10.57ARR135 pKa = 11.84RR136 pKa = 11.84ATQYY140 pKa = 10.97SKK142 pKa = 11.25RR143 pKa = 3.79

Molecular weight: 16.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2936

0

2936

972462

50

4865

331.2

37.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.449 ± 0.047

1.186 ± 0.016

4.712 ± 0.032

6.09 ± 0.05

4.602 ± 0.036

5.937 ± 0.045

2.578 ± 0.022

7.587 ± 0.041

6.46 ± 0.051

10.987 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.02

4.884 ± 0.031

4.09 ± 0.029

4.546 ± 0.033

4.119 ± 0.03

6.552 ± 0.031

5.273 ± 0.029

5.917 ± 0.04

1.129 ± 0.02

3.498 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski