Arthrobacter phage Elesar

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CQJ8|A0A411CQJ8_9CAUD Uncharacterized protein OS=Arthrobacter phage Elesar OX=2510522 GN=61 PE=4 SV=1
MM1 pKa = 6.99TVEE4 pKa = 4.67ALVFPDD10 pKa = 3.33TRR12 pKa = 11.84AALFDD17 pKa = 5.61LIDD20 pKa = 4.07GTTHH24 pKa = 6.78LDD26 pKa = 3.12EE27 pKa = 4.62SVRR30 pKa = 11.84VVYY33 pKa = 10.47RR34 pKa = 11.84LQADD38 pKa = 3.94EE39 pKa = 4.2EE40 pKa = 4.69TGGIKK45 pKa = 10.63GPFPVVHH52 pKa = 6.36VYY54 pKa = 7.96VTGGTSGYY62 pKa = 9.5IDD64 pKa = 3.21RR65 pKa = 11.84VDD67 pKa = 3.33RR68 pKa = 11.84ATIDD72 pKa = 3.32VYY74 pKa = 11.61AEE76 pKa = 4.03GEE78 pKa = 4.03QAVNVLEE85 pKa = 4.84SISASILGSDD95 pKa = 3.28IEE97 pKa = 4.63TPSGYY102 pKa = 10.3LDD104 pKa = 5.51RR105 pKa = 11.84IDD107 pKa = 5.65SDD109 pKa = 3.89VTPADD114 pKa = 3.28VPYY117 pKa = 10.9QSDD120 pKa = 3.56TLNRR124 pKa = 11.84ATATFLVTSRR134 pKa = 11.84PILL137 pKa = 3.72

Molecular weight: 14.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CQL8|A0A411CQL8_9CAUD Uncharacterized protein OS=Arthrobacter phage Elesar OX=2510522 GN=45 PE=4 SV=1
MM1 pKa = 6.32NQEE4 pKa = 4.41RR5 pKa = 11.84ITRR8 pKa = 11.84DD9 pKa = 3.59CEE11 pKa = 4.1CPRR14 pKa = 11.84ANHH17 pKa = 5.2VHH19 pKa = 5.9GTPTAYY25 pKa = 10.72VIDD28 pKa = 3.89KK29 pKa = 9.64CRR31 pKa = 11.84CIPCTEE37 pKa = 4.3ANLKK41 pKa = 10.4RR42 pKa = 11.84EE43 pKa = 4.12QDD45 pKa = 2.8RR46 pKa = 11.84RR47 pKa = 11.84AAKK50 pKa = 10.24KK51 pKa = 10.31AGNYY55 pKa = 8.69DD56 pKa = 3.35AGRR59 pKa = 11.84VDD61 pKa = 4.03AEE63 pKa = 4.29PVRR66 pKa = 11.84HH67 pKa = 6.12HH68 pKa = 6.8IEE70 pKa = 3.68RR71 pKa = 11.84LRR73 pKa = 11.84ARR75 pKa = 11.84GYY77 pKa = 10.39GIKK80 pKa = 10.19QIAKK84 pKa = 9.3LARR87 pKa = 11.84VSNSTLGKK95 pKa = 9.48IVYY98 pKa = 9.89GDD100 pKa = 3.94PSRR103 pKa = 11.84NMPPRR108 pKa = 11.84ARR110 pKa = 11.84VEE112 pKa = 3.74RR113 pKa = 11.84HH114 pKa = 3.76VADD117 pKa = 2.77RR118 pKa = 11.84VMAVRR123 pKa = 11.84PSLSTVGQTTHH134 pKa = 5.96VKK136 pKa = 10.52AGPTQARR143 pKa = 11.84IQSLVCLGYY152 pKa = 10.65SIGWQAIRR160 pKa = 11.84LGKK163 pKa = 10.48VNGNFARR170 pKa = 11.84VLEE173 pKa = 4.33HH174 pKa = 7.62DD175 pKa = 3.85NVNAKK180 pKa = 7.22TARR183 pKa = 11.84EE184 pKa = 3.95VRR186 pKa = 11.84DD187 pKa = 3.99LYY189 pKa = 11.58LLLWDD194 pKa = 5.21KK195 pKa = 10.67PRR197 pKa = 11.84QAANRR202 pKa = 11.84HH203 pKa = 4.37EE204 pKa = 4.27AAAITRR210 pKa = 11.84AIRR213 pKa = 11.84YY214 pKa = 9.37AEE216 pKa = 3.8ARR218 pKa = 11.84GWTEE222 pKa = 3.96LPPPHH227 pKa = 6.9LSGSEE232 pKa = 4.01APVNPDD238 pKa = 2.61KK239 pKa = 11.31GINAFLEE246 pKa = 4.08QRR248 pKa = 11.84LNRR251 pKa = 11.84QTKK254 pKa = 8.15RR255 pKa = 11.84VSRR258 pKa = 11.84EE259 pKa = 3.78SEE261 pKa = 3.87RR262 pKa = 11.84TPWSTTITATNTTSISAA279 pKa = 3.66

Molecular weight: 31.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

13611

53

1607

223.1

24.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.203 ± 0.571

0.808 ± 0.144

5.76 ± 0.265

6.047 ± 0.473

2.792 ± 0.208

8.574 ± 0.525

1.873 ± 0.175

4.32 ± 0.197

3.747 ± 0.234

8.258 ± 0.335

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.954 ± 0.11

3.122 ± 0.318

5.459 ± 0.352

3.798 ± 0.275

6.429 ± 0.335

5.349 ± 0.214

7.016 ± 0.425

7.538 ± 0.25

1.822 ± 0.14

2.131 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski