Streptococcus phage Javan493
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AEF9|A0A4D6AEF9_9CAUD Uncharacterized protein OS=Streptococcus phage Javan493 OX=2548208 GN=Javan493_0044 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.51 NEE4 pKa = 3.97 NFLGYY9 pKa = 10.58 DD10 pKa = 3.33 LAEE13 pKa = 4.29 LLEE16 pKa = 4.39 EE17 pKa = 4.12 KK18 pKa = 10.8 LVGKK22 pKa = 9.58 RR23 pKa = 11.84 LKK25 pKa = 10.98 SIDD28 pKa = 3.63 DD29 pKa = 3.92 EE30 pKa = 5.24 KK31 pKa = 11.12 IVLADD36 pKa = 3.28 GTVIKK41 pKa = 10.6 IEE43 pKa = 4.11 LNEE46 pKa = 4.36 GCGGCGNGWSEE57 pKa = 5.25 LNITTEE63 pKa = 4.07 NPNLEE68 pKa = 4.3 SAVMGVEE75 pKa = 3.92 YY76 pKa = 10.18 TEE78 pKa = 5.91 KK79 pKa = 10.47 YY80 pKa = 10.58 SEE82 pKa = 3.91 WDD84 pKa = 3.42 DD85 pKa = 3.44 EE86 pKa = 4.73 FKK88 pKa = 10.89 IFVYY92 pKa = 8.07 MTDD95 pKa = 2.96 NSVIEE100 pKa = 4.1 IYY102 pKa = 10.77 GYY104 pKa = 10.9 DD105 pKa = 3.49 GVGNGYY111 pKa = 9.81 YY112 pKa = 10.61 GYY114 pKa = 10.67 GFWVTVKK121 pKa = 10.85 NPGDD125 pKa = 4.03 LIEE128 pKa = 5.88 SEE130 pKa = 5.33 LEE132 pKa = 4.05 DD133 pKa = 3.53
Molecular weight: 14.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.812
IPC2_protein 4.024
IPC_protein 3.948
Toseland 3.77
ProMoST 4.024
Dawson 3.897
Bjellqvist 4.088
Wikipedia 3.783
Rodwell 3.783
Grimsley 3.681
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.151
Thurlkill 3.795
EMBOSS 3.795
Sillero 4.062
Patrickios 1.888
IPC_peptide 3.897
IPC2_peptide 4.05
IPC2.peptide.svr19 3.972
Protein with the highest isoelectric point:
>tr|A0A4D6AE31|A0A4D6AE31_9CAUD Uncharacterized protein OS=Streptococcus phage Javan493 OX=2548208 GN=Javan493_0038 PE=4 SV=1
MM1 pKa = 7.15 MVTVSYY7 pKa = 10.18 FSYY10 pKa = 10.7 RR11 pKa = 11.84 DD12 pKa = 3.09 ARR14 pKa = 11.84 QYY16 pKa = 11.27 YY17 pKa = 8.36 EE18 pKa = 4.03 SQITGLRR25 pKa = 11.84 TQLSRR30 pKa = 11.84 TQKK33 pKa = 8.22 QLKK36 pKa = 9.29 RR37 pKa = 11.84 ASEE40 pKa = 3.82 DD41 pKa = 2.95 RR42 pKa = 11.84 ARR44 pKa = 11.84 QTKK47 pKa = 10.1 RR48 pKa = 11.84 IAEE51 pKa = 4.04 MTGNGGG57 pKa = 3.3
Molecular weight: 6.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.882
IPC_protein 10.672
Toseland 10.394
ProMoST 10.204
Dawson 10.599
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 10.76
Grimsley 10.687
Solomon 10.672
Lehninger 10.628
Nozaki 10.35
DTASelect 10.35
Thurlkill 10.452
EMBOSS 10.804
Sillero 10.511
Patrickios 10.496
IPC_peptide 10.672
IPC2_peptide 9.253
IPC2.peptide.svr19 8.415
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
59
0
59
11885
42
1307
201.4
22.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.958 ± 0.652
0.597 ± 0.095
6.714 ± 0.277
7.421 ± 0.319
3.879 ± 0.197
6.487 ± 0.263
1.279 ± 0.115
7.11 ± 0.299
9.028 ± 0.353
8.742 ± 0.265
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.39 ± 0.153
5.646 ± 0.188
2.617 ± 0.18
3.988 ± 0.195
4.047 ± 0.193
6.134 ± 0.305
6.058 ± 0.2
5.982 ± 0.233
1.069 ± 0.125
3.854 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here