Streptococcus phage Javan493

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D6AEF9|A0A4D6AEF9_9CAUD Uncharacterized protein OS=Streptococcus phage Javan493 OX=2548208 GN=Javan493_0044 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.51NEE4 pKa = 3.97NFLGYY9 pKa = 10.58DD10 pKa = 3.33LAEE13 pKa = 4.29LLEE16 pKa = 4.39EE17 pKa = 4.12KK18 pKa = 10.8LVGKK22 pKa = 9.58RR23 pKa = 11.84LKK25 pKa = 10.98SIDD28 pKa = 3.63DD29 pKa = 3.92EE30 pKa = 5.24KK31 pKa = 11.12IVLADD36 pKa = 3.28GTVIKK41 pKa = 10.6IEE43 pKa = 4.11LNEE46 pKa = 4.36GCGGCGNGWSEE57 pKa = 5.25LNITTEE63 pKa = 4.07NPNLEE68 pKa = 4.3SAVMGVEE75 pKa = 3.92YY76 pKa = 10.18TEE78 pKa = 5.91KK79 pKa = 10.47YY80 pKa = 10.58SEE82 pKa = 3.91WDD84 pKa = 3.42DD85 pKa = 3.44EE86 pKa = 4.73FKK88 pKa = 10.89IFVYY92 pKa = 8.07MTDD95 pKa = 2.96NSVIEE100 pKa = 4.1IYY102 pKa = 10.77GYY104 pKa = 10.9DD105 pKa = 3.49GVGNGYY111 pKa = 9.81YY112 pKa = 10.61GYY114 pKa = 10.67GFWVTVKK121 pKa = 10.85NPGDD125 pKa = 4.03LIEE128 pKa = 5.88SEE130 pKa = 5.33LEE132 pKa = 4.05DD133 pKa = 3.53

Molecular weight:
14.98 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D6AE31|A0A4D6AE31_9CAUD Uncharacterized protein OS=Streptococcus phage Javan493 OX=2548208 GN=Javan493_0038 PE=4 SV=1
MM1 pKa = 7.15MVTVSYY7 pKa = 10.18FSYY10 pKa = 10.7RR11 pKa = 11.84DD12 pKa = 3.09ARR14 pKa = 11.84QYY16 pKa = 11.27YY17 pKa = 8.36EE18 pKa = 4.03SQITGLRR25 pKa = 11.84TQLSRR30 pKa = 11.84TQKK33 pKa = 8.22QLKK36 pKa = 9.29RR37 pKa = 11.84ASEE40 pKa = 3.82DD41 pKa = 2.95RR42 pKa = 11.84ARR44 pKa = 11.84QTKK47 pKa = 10.1RR48 pKa = 11.84IAEE51 pKa = 4.04MTGNGGG57 pKa = 3.3

Molecular weight:
6.66 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11885

42

1307

201.4

22.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.958 ± 0.652

0.597 ± 0.095

6.714 ± 0.277

7.421 ± 0.319

3.879 ± 0.197

6.487 ± 0.263

1.279 ± 0.115

7.11 ± 0.299

9.028 ± 0.353

8.742 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.39 ± 0.153

5.646 ± 0.188

2.617 ± 0.18

3.988 ± 0.195

4.047 ± 0.193

6.134 ± 0.305

6.058 ± 0.2

5.982 ± 0.233

1.069 ± 0.125

3.854 ± 0.317

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski