Nocardioides sp. JQ2195

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3770 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H1A056|A0A6H1A056_9ACTN Phospho-sugar mutase OS=Nocardioides sp. JQ2195 OX=2592334 GN=ncot_04225 PE=3 SV=1
MM1 pKa = 7.18HH2 pKa = 6.77SAYY5 pKa = 10.14RR6 pKa = 11.84RR7 pKa = 11.84ASSFRR12 pKa = 11.84QLLARR17 pKa = 11.84MAAVLLISLAAVAGLAGPAAADD39 pKa = 4.5GEE41 pKa = 4.43WSPPDD46 pKa = 3.53GTTSASPSAPADD58 pKa = 3.71DD59 pKa = 5.1AVPAPDD65 pKa = 3.87TPAEE69 pKa = 4.54PEE71 pKa = 4.18TQAPTSAEE79 pKa = 3.95PAAEE83 pKa = 4.03PAEE86 pKa = 4.49EE87 pKa = 4.19PAEE90 pKa = 3.99EE91 pKa = 4.47APTPEE96 pKa = 4.27EE97 pKa = 4.09PKK99 pKa = 10.49AAEE102 pKa = 4.23PKK104 pKa = 10.37AGEE107 pKa = 4.32PADD110 pKa = 3.94EE111 pKa = 4.39QPTPEE116 pKa = 4.66TKK118 pKa = 10.0PAPAKK123 pKa = 9.62STSTTEE129 pKa = 3.49AALAPTTDD137 pKa = 3.73GPEE140 pKa = 4.03SKK142 pKa = 10.58VWICKK147 pKa = 9.9YY148 pKa = 10.83SSTPEE153 pKa = 4.18DD154 pKa = 3.34NEE156 pKa = 3.91KK157 pKa = 10.8AQTVNSVAYY166 pKa = 9.51NSNRR170 pKa = 11.84IPGTWGFEE178 pKa = 4.04DD179 pKa = 4.04AQGLTYY185 pKa = 10.49VYY187 pKa = 10.99SFDD190 pKa = 3.32TGQASKK196 pKa = 10.34PSKK199 pKa = 10.33DD200 pKa = 3.66DD201 pKa = 3.7CPVLIDD207 pKa = 4.48PPAPPTWTEE216 pKa = 3.93PTCADD221 pKa = 3.9PLGSYY226 pKa = 8.3QLPAYY231 pKa = 8.66PADD234 pKa = 4.0RR235 pKa = 11.84ATVSTSGSLEE245 pKa = 3.9PGGTFTVTWTAVSPYY260 pKa = 10.36RR261 pKa = 11.84FLNGSGAPTEE271 pKa = 4.56TISFSHH277 pKa = 6.67TFSQLSSCPVVIDD290 pKa = 4.89DD291 pKa = 4.83LPTVQVSDD299 pKa = 3.74PCGTADD305 pKa = 4.65DD306 pKa = 4.73SVTLSHH312 pKa = 6.84SSQYY316 pKa = 10.76TGVDD320 pKa = 3.3NGDD323 pKa = 3.25GTATFTAVPPNVFPGNEE340 pKa = 3.42ATYY343 pKa = 9.53TVTYY347 pKa = 10.69VPTTDD352 pKa = 3.38VPCIVTVDD360 pKa = 4.11LAAPTITPSTACDD373 pKa = 4.01TPWSIAIGDD382 pKa = 4.0TDD384 pKa = 3.85HH385 pKa = 6.83VSYY388 pKa = 10.6AISPAASGTEE398 pKa = 3.85ATTVTITATPDD409 pKa = 2.9VFFAFSPAPGWTIHH423 pKa = 7.15PDD425 pKa = 2.96GTATFEE431 pKa = 4.3HH432 pKa = 6.73SFAAATTCLDD442 pKa = 3.57VPAEE446 pKa = 4.53PIVTAPTCYY455 pKa = 10.3DD456 pKa = 3.36DD457 pKa = 5.99GYY459 pKa = 9.56LTVEE463 pKa = 4.25IRR465 pKa = 11.84DD466 pKa = 3.79HH467 pKa = 6.21ATVTVNGTTITSATMFGPGSYY488 pKa = 10.48DD489 pKa = 3.25VVYY492 pKa = 9.09TADD495 pKa = 3.64AGHH498 pKa = 6.36VFDD501 pKa = 6.16DD502 pKa = 4.71AGTEE506 pKa = 4.39STTTTYY512 pKa = 11.72DD513 pKa = 4.0DD514 pKa = 3.87IVVEE518 pKa = 4.54AATGDD523 pKa = 4.03CVIDD527 pKa = 4.37VPAQPDD533 pKa = 3.63STDD536 pKa = 2.81TCNPDD541 pKa = 3.27VVVDD545 pKa = 3.93NVEE548 pKa = 3.97WAPFPTLDD556 pKa = 3.94NIVWSVDD563 pKa = 2.83ASTGEE568 pKa = 4.55AIATAINGMSFSNGVTEE585 pKa = 5.05KK586 pKa = 10.47RR587 pKa = 11.84YY588 pKa = 10.45SLPADD593 pKa = 3.51NGIKK597 pKa = 10.09CAAPVDD603 pKa = 4.02PVVTQSSACDD613 pKa = 3.44VQGTLTVPDD622 pKa = 4.09TEE624 pKa = 5.09GITYY628 pKa = 10.69ALDD631 pKa = 4.12GDD633 pKa = 4.73TIAPGTHH640 pKa = 7.26DD641 pKa = 4.43GPLSGTLTATALPGYY656 pKa = 9.81EE657 pKa = 4.56DD658 pKa = 4.42TDD660 pKa = 4.42ADD662 pKa = 3.52WSFHH666 pKa = 5.92VDD668 pKa = 2.81IAAATTCLDD677 pKa = 3.79VPAEE681 pKa = 4.09PAPTPPTCFDD691 pKa = 4.52DD692 pKa = 4.13GTLTVTPAAHH702 pKa = 6.1ATVTVNGDD710 pKa = 3.95PIVDD714 pKa = 3.63EE715 pKa = 4.38TVYY718 pKa = 11.18GPGSYY723 pKa = 10.62DD724 pKa = 3.34VVYY727 pKa = 9.09TADD730 pKa = 3.64AGHH733 pKa = 6.36VFDD736 pKa = 6.16DD737 pKa = 4.71AGTEE741 pKa = 4.39STTTTYY747 pKa = 11.72DD748 pKa = 4.0DD749 pKa = 3.87IVVEE753 pKa = 4.54AATGDD758 pKa = 3.93CVIALEE764 pKa = 4.62AEE766 pKa = 4.06PTEE769 pKa = 4.3IDD771 pKa = 3.8PCNTDD776 pKa = 2.82ATSDD780 pKa = 3.78NIRR783 pKa = 11.84WADD786 pKa = 3.83FPVQDD791 pKa = 5.0DD792 pKa = 4.57IEE794 pKa = 4.4WSVDD798 pKa = 3.12PSTGEE803 pKa = 3.96AVATATNGMFFGPGVTEE820 pKa = 3.74QRR822 pKa = 11.84FAPSPDD828 pKa = 3.47AGLDD832 pKa = 3.55CSLLPGSISSVCVGDD847 pKa = 4.28IPYY850 pKa = 10.55LSYY853 pKa = 11.82AMTLPDD859 pKa = 5.21DD860 pKa = 4.11YY861 pKa = 11.71VGPTDD866 pKa = 3.48MTITFVNPDD875 pKa = 2.74GRR877 pKa = 11.84DD878 pKa = 3.56YY879 pKa = 11.67VVTGQPLAGEE889 pKa = 4.45LLWPGASVDD898 pKa = 3.71PAGWPGWVRR907 pKa = 11.84NPDD910 pKa = 3.09GTYY913 pKa = 10.37SEE915 pKa = 4.61TTGNYY920 pKa = 7.58AWTRR924 pKa = 11.84EE925 pKa = 4.0GVEE928 pKa = 3.77VRR930 pKa = 11.84FQVNPEE936 pKa = 3.78FTTTVAYY943 pKa = 8.65PQATSACAGPAPADD957 pKa = 3.89DD958 pKa = 4.71PGEE961 pKa = 4.35GPEE964 pKa = 4.73EE965 pKa = 4.47GPKK968 pKa = 9.99PQAKK972 pKa = 9.77PSTPDD977 pKa = 3.73AILPNTGAPSGSGMYY992 pKa = 10.21AVLGGLYY999 pKa = 9.12LTFGAWLMFRR1009 pKa = 11.84NRR1011 pKa = 11.84RR1012 pKa = 11.84RR1013 pKa = 11.84RR1014 pKa = 11.84AA1015 pKa = 3.04

Molecular weight: 105.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H1A5L6|A0A6H1A5L6_9ACTN Helix-turn-helix transcriptional regulator OS=Nocardioides sp. JQ2195 OX=2592334 GN=ncot_17925 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.24TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3770

0

3770

1216581

33

2276

322.7

34.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.251 ± 0.048

0.75 ± 0.01

6.687 ± 0.035

6.039 ± 0.038

2.96 ± 0.023

9.017 ± 0.036

2.325 ± 0.02

3.83 ± 0.025

2.393 ± 0.031

9.965 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.026 ± 0.015

2.052 ± 0.022

5.366 ± 0.028

2.849 ± 0.021

7.304 ± 0.043

5.669 ± 0.029

6.044 ± 0.035

9.108 ± 0.034

1.514 ± 0.016

1.852 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski