Mycobacterium phage Patience

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Patiencevirus; Mycobacterium virus Patience

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 110 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1JWH6|G1JWH6_9CAUD Uncharacterized protein OS=Mycobacterium phage Patience OX=1074308 GN=65 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 9.95PIFEE6 pKa = 4.94IIGLIAMAAWLAFCVYY22 pKa = 9.33WGVVLEE28 pKa = 4.39RR29 pKa = 11.84NYY31 pKa = 10.55PVNQKK36 pKa = 9.86EE37 pKa = 4.55DD38 pKa = 3.56NDD40 pKa = 3.84NDD42 pKa = 3.71SEE44 pKa = 4.54YY45 pKa = 11.64

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1JWB2|G1JWB2_9CAUD Uncharacterized protein OS=Mycobacterium phage Patience OX=1074308 GN=1 PE=4 SV=1
MM1 pKa = 7.32ARR3 pKa = 11.84IGRR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.12RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84FSVMTKK17 pKa = 10.32FVSTKK22 pKa = 9.93YY23 pKa = 9.63GSKK26 pKa = 10.27VKK28 pKa = 10.6LVIPGLRR35 pKa = 11.84KK36 pKa = 9.51RR37 pKa = 4.05

Molecular weight: 4.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

110

0

110

22294

31

2215

202.7

22.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.751 ± 0.417

0.875 ± 0.14

6.374 ± 0.196

6.908 ± 0.295

3.701 ± 0.175

7.984 ± 0.318

1.853 ± 0.154

5.822 ± 0.195

6.055 ± 0.424

7.208 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.121

4.571 ± 0.199

4.813 ± 0.175

3.647 ± 0.215

6.311 ± 0.33

5.759 ± 0.176

5.764 ± 0.202

6.486 ± 0.233

1.835 ± 0.132

2.916 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski