Human immunodeficiency virus type 1 group M subtype C (isolate ETH2220) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group M; HIV-1 M:C

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q75009|NEF_HV1ET Protein Nef OS=Human immunodeficiency virus type 1 group M subtype C (isolate ETH2220) OX=388796 GN=nef PE=3 SV=3
MM1 pKa = 7.62GGTMSKK7 pKa = 9.86CSPVGWPAIRR17 pKa = 11.84EE18 pKa = 4.26RR19 pKa = 11.84IRR21 pKa = 11.84RR22 pKa = 11.84AAPAAEE28 pKa = 4.14GVGAASRR35 pKa = 11.84DD36 pKa = 3.4LDD38 pKa = 3.67KK39 pKa = 11.74YY40 pKa = 11.05GALTSSNTPANNPDD54 pKa = 4.12CAWLEE59 pKa = 3.91AQEE62 pKa = 4.4EE63 pKa = 4.48EE64 pKa = 4.71EE65 pKa = 4.28EE66 pKa = 4.33VGFPVRR72 pKa = 11.84PQVPLRR78 pKa = 11.84PMTYY82 pKa = 9.93KK83 pKa = 10.8AAFDD87 pKa = 3.84LSLFLKK93 pKa = 10.48EE94 pKa = 4.21KK95 pKa = 10.76GGLEE99 pKa = 3.66GLIYY103 pKa = 10.31SKK105 pKa = 10.57KK106 pKa = 9.41RR107 pKa = 11.84QEE109 pKa = 5.1ILDD112 pKa = 3.47LWVYY116 pKa = 7.69NTQGFFPDD124 pKa = 3.4WQNYY128 pKa = 5.91TPGPGVRR135 pKa = 11.84YY136 pKa = 8.59PLTFGWCFKK145 pKa = 10.33LVPVDD150 pKa = 3.57PSEE153 pKa = 4.13VEE155 pKa = 4.22EE156 pKa = 4.3INEE159 pKa = 4.26GEE161 pKa = 4.35NNCLLHH167 pKa = 6.33PASLHH172 pKa = 5.46GMEE175 pKa = 5.41DD176 pKa = 3.29EE177 pKa = 4.82DD178 pKa = 6.22RR179 pKa = 11.84EE180 pKa = 4.27VLKK183 pKa = 10.68WKK185 pKa = 10.26FDD187 pKa = 3.23SHH189 pKa = 6.4LARR192 pKa = 11.84RR193 pKa = 11.84HH194 pKa = 4.64MARR197 pKa = 11.84EE198 pKa = 3.9LHH200 pKa = 6.01PEE202 pKa = 4.04YY203 pKa = 11.23YY204 pKa = 10.12KK205 pKa = 11.05DD206 pKa = 3.59CC207 pKa = 4.69

Molecular weight: 23.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q75007|VPU_HV1ET Protein Vpu OS=Human immunodeficiency virus type 1 group M subtype C (isolate ETH2220) OX=388796 GN=vpu PE=3 SV=1
MM1 pKa = 7.87AGRR4 pKa = 11.84SGDD7 pKa = 3.63SDD9 pKa = 4.15EE10 pKa = 4.95EE11 pKa = 3.93LLKK14 pKa = 10.7AVRR17 pKa = 11.84IIKK20 pKa = 9.77ILYY23 pKa = 8.82QSNPYY28 pKa = 7.2PTPEE32 pKa = 3.82GTRR35 pKa = 11.84QARR38 pKa = 11.84RR39 pKa = 11.84NRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84WRR46 pKa = 11.84ARR48 pKa = 11.84QRR50 pKa = 11.84QIHH53 pKa = 5.3TLSEE57 pKa = 4.7RR58 pKa = 11.84ILSNFLGRR66 pKa = 11.84PAEE69 pKa = 4.09PVPLQLPPLEE79 pKa = 4.91RR80 pKa = 11.84LNLDD84 pKa = 3.43CSEE87 pKa = 4.98DD88 pKa = 3.62SGTSGTQQSQGTTEE102 pKa = 4.47GVGNPP107 pKa = 3.79

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

3524

42

1439

391.6

44.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.64 ± 0.556

1.958 ± 0.371

4.37 ± 0.254

6.867 ± 0.591

2.866 ± 0.278

7.123 ± 0.195

2.185 ± 0.723

6.782 ± 0.957

6.952 ± 0.894

8.74 ± 0.656

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.261

4.966 ± 0.608

5.533 ± 0.687

5.817 ± 0.653

5.817 ± 0.942

4.739 ± 0.446

5.704 ± 0.514

5.533 ± 0.425

2.639 ± 0.276

2.44 ± 0.368
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski