Amino acid dipepetide frequency for Nocardiopsis sp. NRRL B-16309

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.738AlaAla: 19.738 ± 0.169
1.021AlaCys: 1.021 ± 0.026
9.0AlaAsp: 9.0 ± 0.08
9.362AlaGlu: 9.362 ± 0.091
3.535AlaPhe: 3.535 ± 0.043
11.712AlaGly: 11.712 ± 0.102
3.042AlaHis: 3.042 ± 0.048
3.562AlaIle: 3.562 ± 0.05
1.982AlaLys: 1.982 ± 0.039
14.494AlaLeu: 14.494 ± 0.13
2.611AlaMet: 2.611 ± 0.043
1.817AlaAsn: 1.817 ± 0.034
7.226AlaPro: 7.226 ± 0.095
3.39AlaGln: 3.39 ± 0.048
10.539AlaArg: 10.539 ± 0.109
5.788AlaSer: 5.788 ± 0.067
6.516AlaThr: 6.516 ± 0.06
12.107AlaVal: 12.107 ± 0.111
1.867AlaTrp: 1.867 ± 0.038
2.421AlaTyr: 2.421 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.039CysAla: 1.039 ± 0.027
0.087CysCys: 0.087 ± 0.009
0.467CysAsp: 0.467 ± 0.015
0.429CysGlu: 0.429 ± 0.015
0.207CysPhe: 0.207 ± 0.011
0.866CysGly: 0.866 ± 0.025
0.192CysHis: 0.192 ± 0.012
0.12CysIle: 0.12 ± 0.008
0.07CysLys: 0.07 ± 0.007
0.683CysLeu: 0.683 ± 0.021
0.111CysMet: 0.111 ± 0.008
0.104CysAsn: 0.104 ± 0.007
0.465CysPro: 0.465 ± 0.018
0.149CysGln: 0.149 ± 0.009
0.533CysArg: 0.533 ± 0.018
0.405CysSer: 0.405 ± 0.015
0.421CysThr: 0.421 ± 0.013
0.682CysVal: 0.682 ± 0.021
0.089CysTrp: 0.089 ± 0.008
0.142CysTyr: 0.142 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.925AspAla: 7.925 ± 0.081
0.387AspCys: 0.387 ± 0.017
4.195AspAsp: 4.195 ± 0.062
4.457AspGlu: 4.457 ± 0.058
1.667AspPhe: 1.667 ± 0.032
6.508AspGly: 6.508 ± 0.086
1.791AspHis: 1.791 ± 0.039
1.906AspIle: 1.906 ± 0.037
0.829AspLys: 0.829 ± 0.025
7.177AspLeu: 7.177 ± 0.082
1.042AspMet: 1.042 ± 0.023
0.924AspAsn: 0.924 ± 0.023
5.105AspPro: 5.105 ± 0.073
1.766AspGln: 1.766 ± 0.03
5.434AspArg: 5.434 ± 0.065
2.753AspSer: 2.753 ± 0.05
3.837AspThr: 3.837 ± 0.064
5.09AspVal: 5.09 ± 0.06
0.974AspTrp: 0.974 ± 0.027
1.293AspTyr: 1.293 ± 0.026
0.0AspXaa: 0.0 ± 0.0
Glu
7.864GluAla: 7.864 ± 0.076
0.381GluCys: 0.381 ± 0.016
3.706GluAsp: 3.706 ± 0.055
4.805GluGlu: 4.805 ± 0.068
1.705GluPhe: 1.705 ± 0.035
5.382GluGly: 5.382 ± 0.057
1.945GluHis: 1.945 ± 0.036
2.428GluIle: 2.428 ± 0.041
1.004GluLys: 1.004 ± 0.029
6.588GluLeu: 6.588 ± 0.071
1.103GluMet: 1.103 ± 0.024
1.154GluAsn: 1.154 ± 0.029
3.858GluPro: 3.858 ± 0.059
2.209GluGln: 2.209 ± 0.044
6.44GluArg: 6.44 ± 0.067
3.113GluSer: 3.113 ± 0.05
2.987GluThr: 2.987 ± 0.047
5.09GluVal: 5.09 ± 0.054
0.89GluTrp: 0.89 ± 0.027
1.254GluTyr: 1.254 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
3.688PheAla: 3.688 ± 0.056
0.243PheCys: 0.243 ± 0.012
2.176PheAsp: 2.176 ± 0.037
1.504PheGlu: 1.504 ± 0.032
0.827PhePhe: 0.827 ± 0.023
3.122PheGly: 3.122 ± 0.044
0.666PheHis: 0.666 ± 0.02
0.655PheIle: 0.655 ± 0.022
0.36PheLys: 0.36 ± 0.015
2.713PheLeu: 2.713 ± 0.047
0.407PheMet: 0.407 ± 0.016
0.537PheAsn: 0.537 ± 0.017
1.418PhePro: 1.418 ± 0.025
0.679PheGln: 0.679 ± 0.019
1.647PheArg: 1.647 ± 0.033
1.486PheSer: 1.486 ± 0.027
2.028PheThr: 2.028 ± 0.033
2.468PheVal: 2.468 ± 0.043
0.418PheTrp: 0.418 ± 0.016
0.561PheTyr: 0.561 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
11.391GlyAla: 11.391 ± 0.103
0.745GlyCys: 0.745 ± 0.024
5.683GlyAsp: 5.683 ± 0.075
5.739GlyGlu: 5.739 ± 0.061
2.837GlyPhe: 2.837 ± 0.047
9.072GlyGly: 9.072 ± 0.11
2.4GlyHis: 2.4 ± 0.045
3.108GlyIle: 3.108 ± 0.043
1.56GlyLys: 1.56 ± 0.038
9.498GlyLeu: 9.498 ± 0.082
2.034GlyMet: 2.034 ± 0.035
1.517GlyAsn: 1.517 ± 0.036
5.402GlyPro: 5.402 ± 0.071
2.486GlyGln: 2.486 ± 0.042
7.81GlyArg: 7.81 ± 0.081
5.198GlySer: 5.198 ± 0.069
6.204GlyThr: 6.204 ± 0.074
8.094GlyVal: 8.094 ± 0.077
1.61GlyTrp: 1.61 ± 0.038
2.256GlyTyr: 2.256 ± 0.043
0.0GlyXaa: 0.0 ± 0.0
His
2.678HisAla: 2.678 ± 0.044
0.173HisCys: 0.173 ± 0.01
1.437HisAsp: 1.437 ± 0.031
1.346HisGlu: 1.346 ± 0.031
0.638HisPhe: 0.638 ± 0.019
2.545HisGly: 2.545 ± 0.04
0.716HisHis: 0.716 ± 0.024
0.638HisIle: 0.638 ± 0.02
0.296HisLys: 0.296 ± 0.013
2.538HisLeu: 2.538 ± 0.045
0.399HisMet: 0.399 ± 0.016
0.399HisAsn: 0.399 ± 0.015
1.754HisPro: 1.754 ± 0.036
0.682HisGln: 0.682 ± 0.02
2.115HisArg: 2.115 ± 0.04
1.05HisSer: 1.05 ± 0.029
1.436HisThr: 1.436 ± 0.03
1.959HisVal: 1.959 ± 0.037
0.377HisTrp: 0.377 ± 0.015
0.49HisTyr: 0.49 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
4.656IleAla: 4.656 ± 0.053
0.227IleCys: 0.227 ± 0.011
2.411IleAsp: 2.411 ± 0.038
2.025IleGlu: 2.025 ± 0.043
0.627IlePhe: 0.627 ± 0.02
3.416IleGly: 3.416 ± 0.055
0.568IleHis: 0.568 ± 0.018
1.019IleIle: 1.019 ± 0.028
0.493IleLys: 0.493 ± 0.018
2.448IleLeu: 2.448 ± 0.05
0.521IleMet: 0.521 ± 0.018
0.692IleAsn: 0.692 ± 0.021
1.668IlePro: 1.668 ± 0.035
0.692IleGln: 0.692 ± 0.021
2.143IleArg: 2.143 ± 0.035
1.598IleSer: 1.598 ± 0.035
2.237IleThr: 2.237 ± 0.034
2.814IleVal: 2.814 ± 0.048
0.318IleTrp: 0.318 ± 0.014
0.485IleTyr: 0.485 ± 0.02
0.0IleXaa: 0.0 ± 0.0
Lys
1.837LysAla: 1.837 ± 0.036
0.079LysCys: 0.079 ± 0.008
0.883LysAsp: 0.883 ± 0.031
0.941LysGlu: 0.941 ± 0.028
0.329LysPhe: 0.329 ± 0.015
1.232LysGly: 1.232 ± 0.031
0.335LysHis: 0.335 ± 0.015
0.584LysIle: 0.584 ± 0.022
0.477LysLys: 0.477 ± 0.025
1.386LysLeu: 1.386 ± 0.031
0.296LysMet: 0.296 ± 0.013
0.334LysAsn: 0.334 ± 0.016
0.868LysPro: 0.868 ± 0.024
0.431LysGln: 0.431 ± 0.019
1.26LysArg: 1.26 ± 0.035
0.806LysSer: 0.806 ± 0.026
0.773LysThr: 0.773 ± 0.025
1.355LysVal: 1.355 ± 0.028
0.179LysTrp: 0.179 ± 0.011
0.3LysTyr: 0.3 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
14.801LeuAla: 14.801 ± 0.132
0.786LeuCys: 0.786 ± 0.023
6.907LeuAsp: 6.907 ± 0.077
5.214LeuGlu: 5.214 ± 0.06
2.828LeuPhe: 2.828 ± 0.046
9.119LeuGly: 9.119 ± 0.086
2.147LeuHis: 2.147 ± 0.035
3.084LeuIle: 3.084 ± 0.045
1.281LeuLys: 1.281 ± 0.034
10.827LeuLeu: 10.827 ± 0.118
1.811LeuMet: 1.811 ± 0.033
1.645LeuAsn: 1.645 ± 0.034
6.238LeuPro: 6.238 ± 0.068
1.785LeuGln: 1.785 ± 0.037
8.842LeuArg: 8.842 ± 0.088
5.688LeuSer: 5.688 ± 0.063
6.348LeuThr: 6.348 ± 0.062
9.687LeuVal: 9.687 ± 0.098
1.394LeuTrp: 1.394 ± 0.032
1.719LeuTyr: 1.719 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.539MetAla: 2.539 ± 0.038
0.149MetCys: 0.149 ± 0.01
1.087MetAsp: 1.087 ± 0.024
0.947MetGlu: 0.947 ± 0.024
0.537MetPhe: 0.537 ± 0.019
1.573MetGly: 1.573 ± 0.033
0.35MetHis: 0.35 ± 0.015
0.685MetIle: 0.685 ± 0.018
0.297MetLys: 0.297 ± 0.013
1.782MetLeu: 1.782 ± 0.036
0.358MetMet: 0.358 ± 0.015
0.406MetAsn: 0.406 ± 0.017
1.13MetPro: 1.13 ± 0.026
0.352MetGln: 0.352 ± 0.014
1.613MetArg: 1.613 ± 0.032
1.361MetSer: 1.361 ± 0.029
1.404MetThr: 1.404 ± 0.029
1.669MetVal: 1.669 ± 0.036
0.242MetTrp: 0.242 ± 0.012
0.323MetTyr: 0.323 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.074AsnAla: 2.074 ± 0.039
0.139AsnCys: 0.139 ± 0.009
0.966AsnAsp: 0.966 ± 0.024
0.938AsnGlu: 0.938 ± 0.026
0.425AsnPhe: 0.425 ± 0.018
1.641AsnGly: 1.641 ± 0.039
0.396AsnHis: 0.396 ± 0.014
0.632AsnIle: 0.632 ± 0.02
0.294AsnLys: 0.294 ± 0.014
1.572AsnLeu: 1.572 ± 0.034
0.298AsnMet: 0.298 ± 0.013
0.376AsnAsn: 0.376 ± 0.014
1.28AsnPro: 1.28 ± 0.028
0.495AsnGln: 0.495 ± 0.018
1.214AsnArg: 1.214 ± 0.029
0.844AsnSer: 0.844 ± 0.029
1.067AsnThr: 1.067 ± 0.025
1.306AsnVal: 1.306 ± 0.03
0.283AsnTrp: 0.283 ± 0.014
0.379AsnTyr: 0.379 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
7.634ProAla: 7.634 ± 0.102
0.307ProCys: 0.307 ± 0.014
4.964ProAsp: 4.964 ± 0.062
5.081ProGlu: 5.081 ± 0.067
1.569ProPhe: 1.569 ± 0.033
6.689ProGly: 6.689 ± 0.08
1.439ProHis: 1.439 ± 0.033
1.389ProIle: 1.389 ± 0.031
0.805ProLys: 0.805 ± 0.025
5.259ProLeu: 5.259 ± 0.055
1.012ProMet: 1.012 ± 0.023
0.854ProAsn: 0.854 ± 0.025
3.576ProPro: 3.576 ± 0.069
1.452ProGln: 1.452 ± 0.038
4.306ProArg: 4.306 ± 0.063
3.216ProSer: 3.216 ± 0.055
3.255ProThr: 3.255 ± 0.052
5.608ProVal: 5.608 ± 0.059
0.995ProTrp: 0.995 ± 0.026
1.168ProTyr: 1.168 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
3.275GlnAla: 3.275 ± 0.051
0.142GlnCys: 0.142 ± 0.01
1.383GlnAsp: 1.383 ± 0.032
1.659GlnGlu: 1.659 ± 0.036
0.579GlnPhe: 0.579 ± 0.017
2.308GlnGly: 2.308 ± 0.044
0.578GlnHis: 0.578 ± 0.02
1.074GlnIle: 1.074 ± 0.027
0.392GlnLys: 0.392 ± 0.016
2.38GlnLeu: 2.38 ± 0.041
0.522GlnMet: 0.522 ± 0.019
0.474GlnAsn: 0.474 ± 0.019
1.272GlnPro: 1.272 ± 0.028
0.845GlnGln: 0.845 ± 0.035
2.256GlnArg: 2.256 ± 0.038
1.153GlnSer: 1.153 ± 0.03
1.2GlnThr: 1.2 ± 0.028
2.418GlnVal: 2.418 ± 0.042
0.42GlnTrp: 0.42 ± 0.017
0.501GlnTyr: 0.501 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
10.44ArgAla: 10.44 ± 0.096
0.522ArgCys: 0.522 ± 0.019
4.773ArgAsp: 4.773 ± 0.062
5.282ArgGlu: 5.282 ± 0.061
2.404ArgPhe: 2.404 ± 0.043
6.373ArgGly: 6.373 ± 0.071
2.028ArgHis: 2.028 ± 0.035
3.036ArgIle: 3.036 ± 0.045
1.292ArgLys: 1.292 ± 0.033
8.703ArgLeu: 8.703 ± 0.095
1.932ArgMet: 1.932 ± 0.038
1.258ArgAsn: 1.258 ± 0.03
5.035ArgPro: 5.035 ± 0.07
2.029ArgGln: 2.029 ± 0.039
8.072ArgArg: 8.072 ± 0.109
4.324ArgSer: 4.324 ± 0.061
5.094ArgThr: 5.094 ± 0.06
7.135ArgVal: 7.135 ± 0.071
1.393ArgTrp: 1.393 ± 0.034
1.776ArgTyr: 1.776 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.787SerAla: 6.787 ± 0.07
0.387SerCys: 0.387 ± 0.016
3.169SerAsp: 3.169 ± 0.059
2.979SerGlu: 2.979 ± 0.05
1.545SerPhe: 1.545 ± 0.031
6.098SerGly: 6.098 ± 0.076
1.044SerHis: 1.044 ± 0.025
1.445SerIle: 1.445 ± 0.031
0.702SerLys: 0.702 ± 0.024
4.843SerLeu: 4.843 ± 0.058
1.093SerMet: 1.093 ± 0.024
0.788SerAsn: 0.788 ± 0.022
3.135SerPro: 3.135 ± 0.049
1.187SerGln: 1.187 ± 0.032
3.759SerArg: 3.759 ± 0.05
2.759SerSer: 2.759 ± 0.055
3.087SerThr: 3.087 ± 0.041
4.56SerVal: 4.56 ± 0.054
0.851SerTrp: 0.851 ± 0.024
1.079SerTyr: 1.079 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
8.158ThrAla: 8.158 ± 0.079
0.416ThrCys: 0.416 ± 0.019
3.903ThrAsp: 3.903 ± 0.055
3.581ThrGlu: 3.581 ± 0.05
1.498ThrPhe: 1.498 ± 0.032
6.645ThrGly: 6.645 ± 0.078
1.228ThrHis: 1.228 ± 0.027
1.751ThrIle: 1.751 ± 0.038
0.811ThrLys: 0.811 ± 0.022
5.367ThrLeu: 5.367 ± 0.062
1.008ThrMet: 1.008 ± 0.025
0.943ThrAsn: 0.943 ± 0.025
3.869ThrPro: 3.869 ± 0.051
1.227ThrGln: 1.227 ± 0.026
4.215ThrArg: 4.215 ± 0.048
2.973ThrSer: 2.973 ± 0.043
3.549ThrThr: 3.549 ± 0.057
5.862ThrVal: 5.862 ± 0.061
0.885ThrTrp: 0.885 ± 0.023
1.128ThrTyr: 1.128 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
11.163ValAla: 11.163 ± 0.1
0.742ValCys: 0.742 ± 0.02
5.714ValAsp: 5.714 ± 0.065
5.502ValGlu: 5.502 ± 0.06
2.73ValPhe: 2.73 ± 0.049
7.269ValGly: 7.269 ± 0.074
2.076ValHis: 2.076 ± 0.033
2.895ValIle: 2.895 ± 0.045
1.169ValLys: 1.169 ± 0.029
10.107ValLeu: 10.107 ± 0.102
1.611ValMet: 1.611 ± 0.035
1.654ValAsn: 1.654 ± 0.036
5.376ValPro: 5.376 ± 0.066
1.921ValGln: 1.921 ± 0.034
7.552ValArg: 7.552 ± 0.082
4.779ValSer: 4.779 ± 0.061
5.387ValThr: 5.387 ± 0.051
8.637ValVal: 8.637 ± 0.1
1.315ValTrp: 1.315 ± 0.032
1.67ValTyr: 1.67 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.678TrpAla: 1.678 ± 0.034
0.149TrpCys: 0.149 ± 0.009
0.91TrpAsp: 0.91 ± 0.025
0.874TrpGlu: 0.874 ± 0.023
0.5TrpPhe: 0.5 ± 0.018
1.124TrpGly: 1.124 ± 0.027
0.358TrpHis: 0.358 ± 0.014
0.582TrpIle: 0.582 ± 0.017
0.251TrpLys: 0.251 ± 0.013
1.61TrpLeu: 1.61 ± 0.037
0.337TrpMet: 0.337 ± 0.014
0.387TrpAsn: 0.387 ± 0.015
0.8TrpPro: 0.8 ± 0.022
0.464TrpGln: 0.464 ± 0.016
1.428TrpArg: 1.428 ± 0.032
1.033TrpSer: 1.033 ± 0.024
0.983TrpThr: 0.983 ± 0.023
1.084TrpVal: 1.084 ± 0.026
0.397TrpTrp: 0.397 ± 0.017
0.286TrpTyr: 0.286 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.368TyrAla: 2.368 ± 0.039
0.176TyrCys: 0.176 ± 0.01
1.325TyrAsp: 1.325 ± 0.032
1.242TyrGlu: 1.242 ± 0.027
0.616TyrPhe: 0.616 ± 0.022
1.949TyrGly: 1.949 ± 0.039
0.367TyrHis: 0.367 ± 0.017
0.468TyrIle: 0.468 ± 0.017
0.322TyrLys: 0.322 ± 0.015
2.179TyrLeu: 2.179 ± 0.037
0.297TyrMet: 0.297 ± 0.013
0.355TyrAsn: 0.355 ± 0.018
1.101TyrPro: 1.101 ± 0.028
0.589TyrGln: 0.589 ± 0.017
1.723TyrArg: 1.723 ± 0.034
0.97TyrSer: 0.97 ± 0.026
1.203TyrThr: 1.203 ± 0.028
1.644TyrVal: 1.644 ± 0.033
0.338TyrTrp: 0.338 ± 0.014
0.459TyrTyr: 0.459 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5253 proteins (1660638 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski