halophilic archaeon J07HX5

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; unclassified Halobacteriales

Average proteome isoelectric point is 5.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2128 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U1MJH6|U1MJH6_9EURY Xaa-pro aminopeptidase OS=halophilic archaeon J07HX5 OX=1325472 GN=J07HX5_01804 PE=4 SV=1
YYY2 pKa = 9.62DD3 pKa = 3.35DD4 pKa = 3.65GGAEEE9 pKa = 4.29GDDD12 pKa = 3.89ATGVEEE18 pKa = 4.53HH19 pKa = 7.2LEEE22 pKa = 4.64EE23 pKa = 4.25YY24 pKa = 10.84DD25 pKa = 4.72IDDD28 pKa = 3.21EE29 pKa = 4.22QIVVYYY35 pKa = 9.63GSYYY39 pKa = 10.52GYYY42 pKa = 6.8TNWQVVQYYY51 pKa = 10.37EEE53 pKa = 5.21YYY55 pKa = 9.84DD56 pKa = 3.66AVTSVGVSDDD66 pKa = 5.21FDDD69 pKa = 3.49YYY71 pKa = 10.91EE72 pKa = 3.62TMPHHH77 pKa = 6.25QTEEE81 pKa = 4.42MVKKK85 pKa = 10.25LGDDD89 pKa = 3.76EEE91 pKa = 4.26EE92 pKa = 4.26ADDD95 pKa = 3.46YYY97 pKa = 10.21EE98 pKa = 4.05EE99 pKa = 4.16SPVEEE104 pKa = 3.61YY105 pKa = 10.73EEE107 pKa = 5.1LACPLLIVHHH117 pKa = 6.58VNDDD121 pKa = 3.45RR122 pKa = 11.84VPVSQARR129 pKa = 11.84ILRR132 pKa = 11.84DDD134 pKa = 3.37EE135 pKa = 4.61EEE137 pKa = 4.34AGFTAGEEE145 pKa = 4.14FEEE148 pKa = 4.11YY149 pKa = 10.94EE150 pKa = 4.08EE151 pKa = 5.1GEEE154 pKa = 4.35EE155 pKa = 4.49HHH157 pKa = 6.56SSDDD161 pKa = 2.93DDD163 pKa = 3.35KKK165 pKa = 10.98RR166 pKa = 11.84SLEEE170 pKa = 4.0LDDD173 pKa = 5.53DD174 pKa = 4.28LDDD177 pKa = 3.88RR178 pKa = 11.84IGTQQAPAVSLDDD191 pKa = 3.58

Molecular weight: 21.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U1MXK0|U1MXK0_9EURY Pyruvate/2-oxoglutarate dehydrogenase complex dehydrogenase (E1) component beta subunit OS=halophilic archaeon J07HX5 OX=1325472 GN=J07HX5_00879 PE=4 SV=1
LL1 pKa = 6.75TPTEE5 pKa = 4.12AVALAHH11 pKa = 7.05DD12 pKa = 4.9RR13 pKa = 11.84GCAAVIAHH21 pKa = 6.85PFRR24 pKa = 11.84RR25 pKa = 11.84SSVADD30 pKa = 3.14SGAAFDD36 pKa = 4.93AVEE39 pKa = 4.27LNGKK43 pKa = 9.4RR44 pKa = 11.84PEE46 pKa = 3.51RR47 pKa = 11.84RR48 pKa = 11.84ARR50 pKa = 11.84VRR52 pKa = 11.84SLAKK56 pKa = 10.48KK57 pKa = 10.27LDD59 pKa = 3.57VPVVGGSDD67 pKa = 3.01AHH69 pKa = 7.76IPVAVGRR76 pKa = 11.84AATRR80 pKa = 11.84IRR82 pKa = 11.84VEE84 pKa = 4.1QPTPAAVVAAIQRR97 pKa = 11.84GDD99 pKa = 3.3VTPVVRR105 pKa = 11.84STPVDD110 pKa = 2.62WMMQRR115 pKa = 11.84CYY117 pKa = 10.28RR118 pKa = 11.84AYY120 pKa = 10.46HH121 pKa = 5.94GAA123 pKa = 3.58

Molecular weight: 13.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2128

0

2128

486033

16

1587

228.4

24.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.515 ± 0.085

0.904 ± 0.022

7.407 ± 0.057

7.273 ± 0.075

2.912 ± 0.033

8.176 ± 0.063

2.025 ± 0.027

4.118 ± 0.043

1.746 ± 0.033

8.829 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.614 ± 0.022

2.474 ± 0.03

4.879 ± 0.039

3.375 ± 0.033

6.844 ± 0.057

5.759 ± 0.05

7.592 ± 0.05

8.771 ± 0.057

1.072 ± 0.021

2.486 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski