Influenza A virus (A/American wigeon/Interior Alaska/10BM05537R0/2010(mixed))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; mixed subtypes

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1SBS6|M1SBS6_9INFA Polymerase acidic protein OS=Influenza A virus (A/American wigeon/Interior Alaska/10BM05537R0/2010(mixed)) OX=1283664 GN=PA PE=3 SV=1
MM1 pKa = 7.38SLLTEE6 pKa = 4.4VEE8 pKa = 4.38TPTRR12 pKa = 11.84NGWEE16 pKa = 4.4CRR18 pKa = 11.84CSDD21 pKa = 3.6SSDD24 pKa = 3.62PLVIAASIIGILHH37 pKa = 7.1LILWILDD44 pKa = 3.39RR45 pKa = 11.84LFFKK49 pKa = 10.78CIYY52 pKa = 9.95RR53 pKa = 11.84RR54 pKa = 11.84LKK56 pKa = 10.55YY57 pKa = 10.03GLKK60 pKa = 10.09RR61 pKa = 11.84GPSTEE66 pKa = 4.02GVPEE70 pKa = 4.24SMRR73 pKa = 11.84EE74 pKa = 3.92EE75 pKa = 4.1YY76 pKa = 10.41RR77 pKa = 11.84QEE79 pKa = 3.74QQSAVDD85 pKa = 3.3VDD87 pKa = 4.47DD88 pKa = 3.92GHH90 pKa = 6.42FVNIEE95 pKa = 3.83LEE97 pKa = 4.08

Molecular weight: 11.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1RR86|M1RR86_9INFA Protein PB1-F2 OS=Influenza A virus (A/American wigeon/Interior Alaska/10BM05537R0/2010(mixed)) OX=1283664 GN=PB1-F2 PE=3 SV=1
MM1 pKa = 7.78ASQGTKK7 pKa = 9.71RR8 pKa = 11.84SYY10 pKa = 10.66EE11 pKa = 3.79QMEE14 pKa = 4.56TGGEE18 pKa = 4.0RR19 pKa = 11.84QNATEE24 pKa = 3.76IRR26 pKa = 11.84ASVGRR31 pKa = 11.84MVGGIGRR38 pKa = 11.84FYY40 pKa = 10.72IQMCTEE46 pKa = 4.87LKK48 pKa = 10.71LSDD51 pKa = 3.6YY52 pKa = 10.66EE53 pKa = 4.36GRR55 pKa = 11.84LIQNSITIEE64 pKa = 4.06RR65 pKa = 11.84MVLSAFDD72 pKa = 3.57EE73 pKa = 4.31RR74 pKa = 11.84RR75 pKa = 11.84NKK77 pKa = 10.12YY78 pKa = 10.45LEE80 pKa = 3.98EE81 pKa = 4.54HH82 pKa = 6.8PSAGKK87 pKa = 10.07DD88 pKa = 3.33PKK90 pKa = 10.14KK91 pKa = 9.42TGGPIYY97 pKa = 10.21RR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84DD101 pKa = 3.68GKK103 pKa = 9.47WMRR106 pKa = 11.84EE107 pKa = 3.73LILYY111 pKa = 9.97DD112 pKa = 3.35KK113 pKa = 11.39EE114 pKa = 3.93EE115 pKa = 3.69IRR117 pKa = 11.84RR118 pKa = 11.84IWRR121 pKa = 11.84QANNGEE127 pKa = 4.33DD128 pKa = 3.23ATAGLTHH135 pKa = 7.15LMIWHH140 pKa = 6.74SNLNDD145 pKa = 3.0ATYY148 pKa = 10.71QRR150 pKa = 11.84TRR152 pKa = 11.84ALVRR156 pKa = 11.84TGMDD160 pKa = 2.98PRR162 pKa = 11.84MCSLMQGSTLPRR174 pKa = 11.84RR175 pKa = 11.84SGAAGAAVKK184 pKa = 10.56GVGTMVMEE192 pKa = 5.61LIRR195 pKa = 11.84MIKK198 pKa = 10.19RR199 pKa = 11.84GINDD203 pKa = 3.34RR204 pKa = 11.84NFWRR208 pKa = 11.84GEE210 pKa = 3.54NGRR213 pKa = 11.84RR214 pKa = 11.84TRR216 pKa = 11.84IAYY219 pKa = 9.45EE220 pKa = 3.95RR221 pKa = 11.84MCNILKK227 pKa = 10.75GKK229 pKa = 9.34FQTAAQRR236 pKa = 11.84AMMDD240 pKa = 3.11QVRR243 pKa = 11.84EE244 pKa = 4.04SRR246 pKa = 11.84NPGNAEE252 pKa = 3.62IEE254 pKa = 4.16DD255 pKa = 4.8LIFLARR261 pKa = 11.84SALILRR267 pKa = 11.84GSVAHH272 pKa = 6.78KK273 pKa = 10.44SCLPACVYY281 pKa = 10.62GLAVASGYY289 pKa = 10.7DD290 pKa = 3.72FEE292 pKa = 6.06RR293 pKa = 11.84EE294 pKa = 4.56GYY296 pKa = 9.53SLVGIDD302 pKa = 5.28PFRR305 pKa = 11.84LLQNSQVFSLIRR317 pKa = 11.84PNEE320 pKa = 4.06NPAHH324 pKa = 6.86KK325 pKa = 10.49SQLVWMACHH334 pKa = 6.25SAAFEE339 pKa = 4.24DD340 pKa = 4.17LRR342 pKa = 11.84VSSFIRR348 pKa = 11.84GTRR351 pKa = 11.84VVPRR355 pKa = 11.84GQLSTRR361 pKa = 11.84GVQIASNEE369 pKa = 4.02NMDD372 pKa = 3.94TMDD375 pKa = 4.26SSTLEE380 pKa = 3.59LRR382 pKa = 11.84SRR384 pKa = 11.84YY385 pKa = 7.55WAIRR389 pKa = 11.84TRR391 pKa = 11.84SGGNTNQQRR400 pKa = 11.84ASAGQISVQPTFSVQRR416 pKa = 11.84NLPFEE421 pKa = 4.38RR422 pKa = 11.84ATIMAAFTGNTEE434 pKa = 3.86GRR436 pKa = 11.84TSDD439 pKa = 3.19MRR441 pKa = 11.84TEE443 pKa = 4.9IIRR446 pKa = 11.84MMEE449 pKa = 3.73NARR452 pKa = 11.84PEE454 pKa = 4.17DD455 pKa = 3.45VSFQGRR461 pKa = 11.84GVFEE465 pKa = 4.95LSDD468 pKa = 3.67EE469 pKa = 4.35KK470 pKa = 10.77ATNPIVPSFDD480 pKa = 3.22MSNEE484 pKa = 3.24GSYY487 pKa = 11.04FFGDD491 pKa = 3.16NAEE494 pKa = 4.48EE495 pKa = 4.16YY496 pKa = 11.1DD497 pKa = 3.62NN498 pKa = 4.9

Molecular weight: 56.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

2

13

5401

90

759

415.5

41.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.073 ± 0.505

1.5 ± 0.303

3.999 ± 0.316

6.573 ± 0.264

3.314 ± 0.214

5.629 ± 0.501

1.518 ± 0.185

5.592 ± 0.374

4.869 ± 0.471

7.202 ± 0.556

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.499 ± 0.513

4.906 ± 0.584

3.333 ± 0.376

4.092 ± 0.565

6.258 ± 0.735

6.406 ± 0.527

6.221 ± 0.531

4.888 ± 0.563

1.315 ± 0.191

2.222 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski