Amino acid dipepetide frequency for Spirochaeta sp. LUC14_002_19_P3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.997AlaAla: 11.997 ± 0.238
0.932AlaCys: 0.932 ± 0.044
5.229AlaAsp: 5.229 ± 0.106
6.666AlaGlu: 6.666 ± 0.11
3.273AlaPhe: 3.273 ± 0.086
7.483AlaGly: 7.483 ± 0.139
1.477AlaHis: 1.477 ± 0.052
5.055AlaIle: 5.055 ± 0.112
3.734AlaLys: 3.734 ± 0.101
10.505AlaLeu: 10.505 ± 0.173
2.339AlaMet: 2.339 ± 0.073
2.838AlaAsn: 2.838 ± 0.079
3.068AlaPro: 3.068 ± 0.084
2.755AlaGln: 2.755 ± 0.073
5.084AlaArg: 5.084 ± 0.121
5.071AlaSer: 5.071 ± 0.088
2.661AlaThr: 2.661 ± 0.081
7.387AlaVal: 7.387 ± 0.122
1.214AlaTrp: 1.214 ± 0.047
2.456AlaTyr: 2.456 ± 0.07
0.0AlaXaa: 0.0 ± 0.0
Cys
0.876CysAla: 0.876 ± 0.04
0.159CysCys: 0.159 ± 0.017
0.449CysAsp: 0.449 ± 0.029
0.464CysGlu: 0.464 ± 0.029
0.414CysPhe: 0.414 ± 0.025
1.063CysGly: 1.063 ± 0.05
0.234CysHis: 0.234 ± 0.02
0.568CysIle: 0.568 ± 0.034
0.333CysLys: 0.333 ± 0.024
0.938CysLeu: 0.938 ± 0.042
0.207CysMet: 0.207 ± 0.02
0.237CysAsn: 0.237 ± 0.021
0.58CysPro: 0.58 ± 0.035
0.23CysGln: 0.23 ± 0.02
0.851CysArg: 0.851 ± 0.037
0.694CysSer: 0.694 ± 0.031
0.48CysThr: 0.48 ± 0.029
0.439CysVal: 0.439 ± 0.028
0.14CysTrp: 0.14 ± 0.016
0.301CysTyr: 0.301 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
4.673AspAla: 4.673 ± 0.1
0.508AspCys: 0.508 ± 0.031
2.398AspAsp: 2.398 ± 0.079
3.792AspGlu: 3.792 ± 0.092
2.385AspPhe: 2.385 ± 0.066
3.933AspGly: 3.933 ± 0.11
0.587AspHis: 0.587 ± 0.033
4.418AspIle: 4.418 ± 0.11
3.07AspLys: 3.07 ± 0.081
4.187AspLeu: 4.187 ± 0.096
1.472AspMet: 1.472 ± 0.049
2.114AspAsn: 2.114 ± 0.072
2.01AspPro: 2.01 ± 0.056
0.994AspGln: 0.994 ± 0.043
2.543AspArg: 2.543 ± 0.074
3.558AspSer: 3.558 ± 0.085
3.042AspThr: 3.042 ± 0.085
2.84AspVal: 2.84 ± 0.072
0.865AspTrp: 0.865 ± 0.038
2.137AspTyr: 2.137 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
6.382GluAla: 6.382 ± 0.141
0.536GluCys: 0.536 ± 0.032
3.493GluAsp: 3.493 ± 0.086
5.427GluGlu: 5.427 ± 0.124
2.566GluPhe: 2.566 ± 0.077
4.565GluGly: 4.565 ± 0.091
1.267GluHis: 1.267 ± 0.051
4.691GluIle: 4.691 ± 0.11
4.563GluLys: 4.563 ± 0.116
6.74GluLeu: 6.74 ± 0.13
1.637GluMet: 1.637 ± 0.053
3.44GluAsn: 3.44 ± 0.074
2.07GluPro: 2.07 ± 0.062
2.419GluGln: 2.419 ± 0.068
3.739GluArg: 3.739 ± 0.085
3.741GluSer: 3.741 ± 0.095
3.528GluThr: 3.528 ± 0.086
3.765GluVal: 3.765 ± 0.093
0.899GluTrp: 0.899 ± 0.045
2.083GluTyr: 2.083 ± 0.066
0.0GluXaa: 0.0 ± 0.0
Phe
3.252PheAla: 3.252 ± 0.082
0.432PheCys: 0.432 ± 0.028
2.185PheAsp: 2.185 ± 0.063
2.187PheGlu: 2.187 ± 0.067
2.067PhePhe: 2.067 ± 0.071
3.093PheGly: 3.093 ± 0.074
0.931PheHis: 0.931 ± 0.043
3.036PheIle: 3.036 ± 0.08
1.801PheLys: 1.801 ± 0.067
4.155PheLeu: 4.155 ± 0.11
0.996PheMet: 0.996 ± 0.047
1.752PheAsn: 1.752 ± 0.065
2.008PhePro: 2.008 ± 0.063
1.398PheGln: 1.398 ± 0.044
2.405PheArg: 2.405 ± 0.066
3.493PheSer: 3.493 ± 0.088
2.628PheThr: 2.628 ± 0.082
2.088PheVal: 2.088 ± 0.061
0.499PheTrp: 0.499 ± 0.033
1.423PheTyr: 1.423 ± 0.048
0.0PheXaa: 0.0 ± 0.0
Gly
6.266GlyAla: 6.266 ± 0.132
0.977GlyCys: 0.977 ± 0.047
3.406GlyAsp: 3.406 ± 0.078
5.153GlyGlu: 5.153 ± 0.111
3.493GlyPhe: 3.493 ± 0.09
6.19GlyGly: 6.19 ± 0.148
1.389GlyHis: 1.389 ± 0.053
6.073GlyIle: 6.073 ± 0.12
4.604GlyLys: 4.604 ± 0.097
7.4GlyLeu: 7.4 ± 0.144
2.493GlyMet: 2.493 ± 0.074
2.953GlyAsn: 2.953 ± 0.076
2.058GlyPro: 2.058 ± 0.061
2.049GlyGln: 2.049 ± 0.06
4.62GlyArg: 4.62 ± 0.124
4.832GlySer: 4.832 ± 0.103
4.638GlyThr: 4.638 ± 0.117
4.539GlyVal: 4.539 ± 0.099
1.099GlyTrp: 1.099 ± 0.044
2.444GlyTyr: 2.444 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
1.398HisAla: 1.398 ± 0.055
0.239HisCys: 0.239 ± 0.022
0.78HisAsp: 0.78 ± 0.04
1.09HisGlu: 1.09 ± 0.039
0.872HisPhe: 0.872 ± 0.038
1.555HisGly: 1.555 ± 0.056
0.423HisHis: 0.423 ± 0.028
1.292HisIle: 1.292 ± 0.047
0.934HisLys: 0.934 ± 0.043
2.081HisLeu: 2.081 ± 0.061
0.17HisMet: 0.17 ± 0.016
0.763HisAsn: 0.763 ± 0.035
1.186HisPro: 1.186 ± 0.051
0.586HisGln: 0.586 ± 0.034
1.147HisArg: 1.147 ± 0.044
1.228HisSer: 1.228 ± 0.052
0.945HisThr: 0.945 ± 0.043
0.932HisVal: 0.932 ± 0.043
0.267HisTrp: 0.267 ± 0.021
0.805HisTyr: 0.805 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
6.674IleAla: 6.674 ± 0.11
0.602IleCys: 0.602 ± 0.035
3.788IleAsp: 3.788 ± 0.085
4.043IleGlu: 4.043 ± 0.091
2.751IlePhe: 2.751 ± 0.075
4.839IleGly: 4.839 ± 0.1
1.543IleHis: 1.543 ± 0.058
5.002IleIle: 5.002 ± 0.117
3.222IleLys: 3.222 ± 0.094
7.025IleLeu: 7.025 ± 0.135
1.405IleMet: 1.405 ± 0.053
2.513IleAsn: 2.513 ± 0.075
3.787IlePro: 3.787 ± 0.099
2.261IleGln: 2.261 ± 0.062
3.895IleArg: 3.895 ± 0.105
4.839IleSer: 4.839 ± 0.109
3.749IleThr: 3.749 ± 0.093
3.953IleVal: 3.953 ± 0.088
0.653IleTrp: 0.653 ± 0.037
1.9IleTyr: 1.9 ± 0.068
0.0IleXaa: 0.0 ± 0.0
Lys
4.662LysAla: 4.662 ± 0.105
0.347LysCys: 0.347 ± 0.027
2.882LysAsp: 2.882 ± 0.073
3.714LysGlu: 3.714 ± 0.105
1.545LysPhe: 1.545 ± 0.06
3.588LysGly: 3.588 ± 0.102
1.021LysHis: 1.021 ± 0.039
3.741LysIle: 3.741 ± 0.091
3.703LysLys: 3.703 ± 0.096
4.898LysLeu: 4.898 ± 0.105
1.246LysMet: 1.246 ± 0.048
2.785LysAsn: 2.785 ± 0.079
2.192LysPro: 2.192 ± 0.075
1.787LysGln: 1.787 ± 0.058
2.842LysArg: 2.842 ± 0.083
3.098LysSer: 3.098 ± 0.07
3.342LysThr: 3.342 ± 0.092
3.121LysVal: 3.121 ± 0.083
0.533LysTrp: 0.533 ± 0.029
1.433LysTyr: 1.433 ± 0.048
0.0LysXaa: 0.0 ± 0.0
Leu
9.582LeuAla: 9.582 ± 0.155
1.067LeuCys: 1.067 ± 0.043
5.253LeuAsp: 5.253 ± 0.106
6.835LeuGlu: 6.835 ± 0.133
4.358LeuPhe: 4.358 ± 0.107
7.234LeuGly: 7.234 ± 0.146
1.932LeuHis: 1.932 ± 0.064
6.598LeuIle: 6.598 ± 0.116
6.05LeuLys: 6.05 ± 0.117
10.581LeuLeu: 10.581 ± 0.202
2.348LeuMet: 2.348 ± 0.074
4.475LeuAsn: 4.475 ± 0.103
5.298LeuPro: 5.298 ± 0.114
2.838LeuGln: 2.838 ± 0.072
5.795LeuArg: 5.795 ± 0.113
7.741LeuSer: 7.741 ± 0.132
5.657LeuThr: 5.657 ± 0.12
5.361LeuVal: 5.361 ± 0.109
1.101LeuTrp: 1.101 ± 0.054
3.105LeuTyr: 3.105 ± 0.084
0.0LeuXaa: 0.0 ± 0.0
Met
2.702MetAla: 2.702 ± 0.071
0.115MetCys: 0.115 ± 0.015
1.561MetAsp: 1.561 ± 0.05
2.019MetGlu: 2.019 ± 0.065
0.685MetPhe: 0.685 ± 0.034
1.755MetGly: 1.755 ± 0.061
0.313MetHis: 0.313 ± 0.02
1.371MetIle: 1.371 ± 0.051
1.861MetLys: 1.861 ± 0.058
2.345MetLeu: 2.345 ± 0.062
0.566MetMet: 0.566 ± 0.035
1.313MetAsn: 1.313 ± 0.05
1.177MetPro: 1.177 ± 0.044
0.733MetGln: 0.733 ± 0.037
1.37MetArg: 1.37 ± 0.062
1.477MetSer: 1.477 ± 0.053
0.968MetThr: 0.968 ± 0.042
1.467MetVal: 1.467 ± 0.058
0.122MetTrp: 0.122 ± 0.018
0.483MetTyr: 0.483 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
3.27AsnAla: 3.27 ± 0.089
0.398AsnCys: 0.398 ± 0.029
1.69AsnAsp: 1.69 ± 0.053
2.238AsnGlu: 2.238 ± 0.076
1.824AsnPhe: 1.824 ± 0.058
2.861AsnGly: 2.861 ± 0.073
0.695AsnHis: 0.695 ± 0.041
3.128AsnIle: 3.128 ± 0.086
1.946AsnLys: 1.946 ± 0.071
4.201AsnLeu: 4.201 ± 0.086
0.966AsnMet: 0.966 ± 0.042
1.656AsnAsn: 1.656 ± 0.074
2.721AsnPro: 2.721 ± 0.075
1.35AsnGln: 1.35 ± 0.055
2.337AsnArg: 2.337 ± 0.079
2.705AsnSer: 2.705 ± 0.071
2.327AsnThr: 2.327 ± 0.073
1.945AsnVal: 1.945 ± 0.064
0.635AsnTrp: 0.635 ± 0.036
1.366AsnTyr: 1.366 ± 0.052
0.0AsnXaa: 0.0 ± 0.0
Pro
4.045ProAla: 4.045 ± 0.108
0.373ProCys: 0.373 ± 0.029
2.808ProAsp: 2.808 ± 0.079
3.898ProGlu: 3.898 ± 0.086
1.87ProPhe: 1.87 ± 0.066
3.964ProGly: 3.964 ± 0.109
0.839ProHis: 0.839 ± 0.04
2.318ProIle: 2.318 ± 0.064
1.729ProLys: 1.729 ± 0.051
4.624ProLeu: 4.624 ± 0.106
1.009ProMet: 1.009 ± 0.042
1.578ProAsn: 1.578 ± 0.053
2.256ProPro: 2.256 ± 0.08
1.467ProGln: 1.467 ± 0.051
2.077ProArg: 2.077 ± 0.071
2.612ProSer: 2.612 ± 0.074
1.654ProThr: 1.654 ± 0.054
3.305ProVal: 3.305 ± 0.08
0.692ProTrp: 0.692 ± 0.043
1.41ProTyr: 1.41 ± 0.057
0.0ProXaa: 0.0 ± 0.0
Gln
2.948GlnAla: 2.948 ± 0.076
0.212GlnCys: 0.212 ± 0.019
1.421GlnAsp: 1.421 ± 0.053
2.06GlnGlu: 2.06 ± 0.068
1.347GlnPhe: 1.347 ± 0.046
2.175GlnGly: 2.175 ± 0.061
0.557GlnHis: 0.557 ± 0.035
2.063GlnIle: 2.063 ± 0.061
1.761GlnLys: 1.761 ± 0.058
2.928GlnLeu: 2.928 ± 0.078
0.825GlnMet: 0.825 ± 0.041
1.338GlnAsn: 1.338 ± 0.052
1.216GlnPro: 1.216 ± 0.042
1.147GlnGln: 1.147 ± 0.054
1.9GlnArg: 1.9 ± 0.066
2.042GlnSer: 2.042 ± 0.069
1.605GlnThr: 1.605 ± 0.056
1.881GlnVal: 1.881 ± 0.051
0.451GlnTrp: 0.451 ± 0.031
1.069GlnTyr: 1.069 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
4.312ArgAla: 4.312 ± 0.102
0.591ArgCys: 0.591 ± 0.034
2.985ArgAsp: 2.985 ± 0.081
4.834ArgGlu: 4.834 ± 0.118
2.585ArgPhe: 2.585 ± 0.083
4.181ArgGly: 4.181 ± 0.083
1.223ArgHis: 1.223 ± 0.046
4.431ArgIle: 4.431 ± 0.096
3.093ArgLys: 3.093 ± 0.081
6.167ArgLeu: 6.167 ± 0.117
1.493ArgMet: 1.493 ± 0.053
2.201ArgAsn: 2.201 ± 0.058
2.031ArgPro: 2.031 ± 0.071
2.114ArgGln: 2.114 ± 0.073
4.383ArgArg: 4.383 ± 0.112
3.104ArgSer: 3.104 ± 0.062
2.642ArgThr: 2.642 ± 0.072
3.394ArgVal: 3.394 ± 0.077
0.766ArgTrp: 0.766 ± 0.04
2.017ArgTyr: 2.017 ± 0.068
0.0ArgXaa: 0.0 ± 0.0
Ser
5.738SerAla: 5.738 ± 0.105
0.676SerCys: 0.676 ± 0.035
3.185SerAsp: 3.185 ± 0.083
3.769SerGlu: 3.769 ± 0.086
2.854SerPhe: 2.854 ± 0.07
6.377SerGly: 6.377 ± 0.119
1.187SerHis: 1.187 ± 0.049
4.344SerIle: 4.344 ± 0.097
2.548SerLys: 2.548 ± 0.075
7.088SerLeu: 7.088 ± 0.126
1.635SerMet: 1.635 ± 0.053
2.017SerAsn: 2.017 ± 0.066
3.176SerPro: 3.176 ± 0.09
1.846SerGln: 1.846 ± 0.057
4.5SerArg: 4.5 ± 0.099
4.516SerSer: 4.516 ± 0.113
3.194SerThr: 3.194 ± 0.082
3.767SerVal: 3.767 ± 0.082
0.94SerTrp: 0.94 ± 0.043
1.976SerTyr: 1.976 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
5.698ThrAla: 5.698 ± 0.14
0.368ThrCys: 0.368 ± 0.024
2.916ThrAsp: 2.916 ± 0.092
3.017ThrGlu: 3.017 ± 0.085
1.831ThrPhe: 1.831 ± 0.066
4.756ThrGly: 4.756 ± 0.088
0.876ThrHis: 0.876 ± 0.04
3.341ThrIle: 3.341 ± 0.089
1.716ThrLys: 1.716 ± 0.053
5.731ThrLeu: 5.731 ± 0.097
1.101ThrMet: 1.101 ± 0.046
1.582ThrAsn: 1.582 ± 0.061
2.559ThrPro: 2.559 ± 0.082
1.361ThrGln: 1.361 ± 0.053
2.488ThrArg: 2.488 ± 0.067
2.974ThrSer: 2.974 ± 0.089
2.072ThrThr: 2.072 ± 0.086
4.047ThrVal: 4.047 ± 0.107
0.61ThrTrp: 0.61 ± 0.033
1.347ThrTyr: 1.347 ± 0.058
0.0ThrXaa: 0.0 ± 0.0
Val
3.507ValAla: 3.507 ± 0.104
0.667ValCys: 0.667 ± 0.036
2.934ValAsp: 2.934 ± 0.079
3.702ValGlu: 3.702 ± 0.09
3.118ValPhe: 3.118 ± 0.076
3.712ValGly: 3.712 ± 0.085
1.226ValHis: 1.226 ± 0.049
4.289ValIle: 4.289 ± 0.091
3.511ValLys: 3.511 ± 0.087
6.814ValLeu: 6.814 ± 0.114
1.479ValMet: 1.479 ± 0.054
2.718ValAsn: 2.718 ± 0.08
2.994ValPro: 2.994 ± 0.075
1.938ValGln: 1.938 ± 0.054
3.488ValArg: 3.488 ± 0.079
4.609ValSer: 4.609 ± 0.109
2.773ValThr: 2.773 ± 0.088
3.445ValVal: 3.445 ± 0.083
0.702ValTrp: 0.702 ± 0.034
2.164ValTyr: 2.164 ± 0.07
0.0ValXaa: 0.0 ± 0.0
Trp
0.848TrpAla: 0.848 ± 0.041
0.156TrpCys: 0.156 ± 0.016
0.664TrpAsp: 0.664 ± 0.033
0.906TrpGlu: 0.906 ± 0.041
0.538TrpPhe: 0.538 ± 0.033
0.853TrpGly: 0.853 ± 0.038
0.272TrpHis: 0.272 ± 0.022
0.756TrpIle: 0.756 ± 0.037
0.798TrpLys: 0.798 ± 0.039
1.499TrpLeu: 1.499 ± 0.05
0.354TrpMet: 0.354 ± 0.026
0.734TrpAsn: 0.734 ± 0.035
0.524TrpPro: 0.524 ± 0.035
0.538TrpGln: 0.538 ± 0.036
0.805TrpArg: 0.805 ± 0.038
0.715TrpSer: 0.715 ± 0.039
0.619TrpThr: 0.619 ± 0.036
0.633TrpVal: 0.633 ± 0.036
0.186TrpTrp: 0.186 ± 0.021
0.478TrpTyr: 0.478 ± 0.03
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.396TyrAla: 2.396 ± 0.065
0.301TyrCys: 0.301 ± 0.023
1.5TyrAsp: 1.5 ± 0.056
1.738TyrGlu: 1.738 ± 0.062
1.451TyrPhe: 1.451 ± 0.05
2.495TyrGly: 2.495 ± 0.067
0.699TyrHis: 0.699 ± 0.037
1.93TyrIle: 1.93 ± 0.06
1.504TyrLys: 1.504 ± 0.051
3.305TyrLeu: 3.305 ± 0.088
0.66TyrMet: 0.66 ± 0.032
1.255TyrAsn: 1.255 ± 0.05
1.617TyrPro: 1.617 ± 0.064
1.134TyrGln: 1.134 ± 0.044
2.281TyrArg: 2.281 ± 0.071
2.283TyrSer: 2.283 ± 0.063
1.867TyrThr: 1.867 ± 0.065
1.481TyrVal: 1.481 ± 0.051
0.501TyrTrp: 0.501 ± 0.032
1.239TyrTyr: 1.239 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1687 proteins (565149 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski