Hubei narna-like virus 24
Average proteome isoelectric point is 8.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIB4|A0A1L3KIB4_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 24 OX=1922955 PE=4 SV=1
MM1 pKa = 8.13 DD2 pKa = 3.55 NTITHH7 pKa = 7.21 ALCYY11 pKa = 9.64 AANITLGKK19 pKa = 9.63 DD20 pKa = 2.94 AKK22 pKa = 10.55 PFIEE26 pKa = 5.11 RR27 pKa = 11.84 IEE29 pKa = 4.45 HH30 pKa = 5.7 FLSHH34 pKa = 6.87 NGTEE38 pKa = 4.24 YY39 pKa = 10.37 TVNRR43 pKa = 11.84 LKK45 pKa = 10.9 VIYY48 pKa = 10.05 QGALLLRR55 pKa = 11.84 EE56 pKa = 4.12 YY57 pKa = 10.51 DD58 pKa = 3.47 QLGRR62 pKa = 11.84 PVDD65 pKa = 3.64 TDD67 pKa = 3.64 FLGNTNRR74 pKa = 11.84 GSFDD78 pKa = 3.42 QAVKK82 pKa = 10.4 LYY84 pKa = 9.92 HH85 pKa = 6.63 EE86 pKa = 4.85 NGIATSKK93 pKa = 10.46 KK94 pKa = 10.25 GYY96 pKa = 9.24 PKK98 pKa = 10.51 DD99 pKa = 3.29 STGIVVRR106 pKa = 11.84 QFVEE110 pKa = 4.25 SNRR113 pKa = 11.84 PQVIRR118 pKa = 11.84 NMTGLLRR125 pKa = 11.84 SYY127 pKa = 9.11 TQFRR131 pKa = 11.84 LDD133 pKa = 3.75 SVSQQQVDD141 pKa = 3.74 KK142 pKa = 10.87 VVEE145 pKa = 4.56 SVCMPRR151 pKa = 11.84 DD152 pKa = 3.32 RR153 pKa = 11.84 AHH155 pKa = 7.77 DD156 pKa = 3.87 EE157 pKa = 3.92 NQLFDD162 pKa = 4.22 NLIHH166 pKa = 7.35 LSRR169 pKa = 11.84 VPKK172 pKa = 10.51 ALWNKK177 pKa = 9.46 SIKK180 pKa = 10.37 AEE182 pKa = 4.04 RR183 pKa = 11.84 LSMDD187 pKa = 3.78 CLRR190 pKa = 11.84 PYY192 pKa = 10.09 TSVYY196 pKa = 10.41 CHH198 pKa = 6.54 GSVPKK203 pKa = 10.15 HH204 pKa = 6.25 VKK206 pKa = 10.29 DD207 pKa = 3.73 EE208 pKa = 4.6 PYY210 pKa = 11.12 GKK212 pKa = 10.11 ALYY215 pKa = 11.24 SMITTSYY222 pKa = 10.37 LPSPLEE228 pKa = 3.81 SVVPSKK234 pKa = 10.77 SIRR237 pKa = 11.84 DD238 pKa = 3.73 RR239 pKa = 11.84 VRR241 pKa = 11.84 SWMPRR246 pKa = 11.84 EE247 pKa = 3.86 NDD249 pKa = 3.38 YY250 pKa = 11.59 DD251 pKa = 3.76 PEE253 pKa = 4.1 QYY255 pKa = 10.42 AGKK258 pKa = 9.5 IHH260 pKa = 7.37 IIQEE264 pKa = 4.07 AGAKK268 pKa = 9.44 ARR270 pKa = 11.84 TVAIPNGWTQIGFVPLHH287 pKa = 6.78 DD288 pKa = 3.84 QMQLEE293 pKa = 4.47 SEE295 pKa = 4.56 RR296 pKa = 11.84 YY297 pKa = 8.48 FPSEE301 pKa = 3.82 SCVFDD306 pKa = 3.59 QLKK309 pKa = 10.67 GIYY312 pKa = 10.0 ALRR315 pKa = 11.84 NHH317 pKa = 6.4 MGDD320 pKa = 2.55 GKK322 pKa = 9.21 TPVSVDD328 pKa = 3.47 LSSATDD334 pKa = 3.6 RR335 pKa = 11.84 FPIEE339 pKa = 4.25 FQCKK343 pKa = 8.47 LLSHH347 pKa = 7.12 IGLPFYY353 pKa = 10.94 SDD355 pKa = 3.51 SLSEE359 pKa = 4.05 LVKK362 pKa = 10.9 KK363 pKa = 10.07 PWEE366 pKa = 4.31 FPQGKK371 pKa = 8.37 PFGYY375 pKa = 10.66 DD376 pKa = 2.66 SVYY379 pKa = 11.09 YY380 pKa = 10.54 NAGQPMGLYY389 pKa = 10.38 GSFPTFHH396 pKa = 7.21 FSNLVMADD404 pKa = 3.75 ASCRR408 pKa = 11.84 LTDD411 pKa = 3.64 YY412 pKa = 11.18 QIGEE416 pKa = 4.43 TIDD419 pKa = 3.83 GKK421 pKa = 11.2 GKK423 pKa = 9.31 EE424 pKa = 4.16 QVAGIVYY431 pKa = 8.65 EE432 pKa = 4.03 RR433 pKa = 11.84 LYY435 pKa = 11.24 GFYY438 pKa = 10.75 AHH440 pKa = 7.66 RR441 pKa = 11.84 DD442 pKa = 3.32 KK443 pKa = 11.34 DD444 pKa = 4.33 TVPDD448 pKa = 4.73 DD449 pKa = 4.04 YY450 pKa = 11.45 IEE452 pKa = 4.47 RR453 pKa = 11.84 NNILGNAAGDD463 pKa = 4.01 SPVSKK468 pKa = 10.26 FYY470 pKa = 10.97 DD471 pKa = 3.5 GSRR474 pKa = 11.84 FKK476 pKa = 11.36 VLGDD480 pKa = 4.48 DD481 pKa = 3.96 IVFSDD486 pKa = 4.05 EE487 pKa = 4.18 RR488 pKa = 11.84 DD489 pKa = 3.24 AAHH492 pKa = 5.73 YY493 pKa = 7.92 TKK495 pKa = 10.39 IMKK498 pKa = 8.63 TINVPISHH506 pKa = 6.87 HH507 pKa = 6.26 KK508 pKa = 10.31 SFSKK512 pKa = 10.9 GMVCEE517 pKa = 4.15 FAGAIAMRR525 pKa = 11.84 TKK527 pKa = 10.62 KK528 pKa = 10.46 SNKK531 pKa = 8.4 DD532 pKa = 2.88 RR533 pKa = 11.84 RR534 pKa = 11.84 YY535 pKa = 9.77 DD536 pKa = 3.37 VSIFRR541 pKa = 11.84 PYY543 pKa = 10.76 KK544 pKa = 9.47 FPKK547 pKa = 10.14 DD548 pKa = 3.26 GSFIGNPISFIYY560 pKa = 10.35 AFSSPQSKK568 pKa = 9.76 QRR570 pKa = 11.84 VSKK573 pKa = 9.95 KK574 pKa = 7.69 WKK576 pKa = 9.99 QFFEE580 pKa = 5.47 DD581 pKa = 3.79 FDD583 pKa = 3.53 KK584 pKa = 11.1 TRR586 pKa = 11.84 PQRR589 pKa = 11.84 YY590 pKa = 9.4 ADD592 pKa = 4.31 LCPITPFVEE601 pKa = 4.0 DD602 pKa = 2.75 RR603 pKa = 11.84 VGIVGRR609 pKa = 11.84 FRR611 pKa = 11.84 DD612 pKa = 3.54 QDD614 pKa = 2.83 IDD616 pKa = 3.78 RR617 pKa = 11.84 VVDD620 pKa = 3.99 NIYY623 pKa = 10.63 RR624 pKa = 11.84 FSNLSFFPYY633 pKa = 10.19 TDD635 pKa = 3.22 TGKK638 pKa = 8.81 DD639 pKa = 3.13 TPIIYY644 pKa = 9.87 DD645 pKa = 3.7 GPRR648 pKa = 11.84 NRR650 pKa = 11.84 APLYY654 pKa = 9.31 QVPLFIKK661 pKa = 10.26 KK662 pKa = 10.13 KK663 pKa = 8.82 GIEE666 pKa = 4.03 VPGFEE671 pKa = 4.43 TQRR674 pKa = 11.84 PASDD678 pKa = 2.92 TRR680 pKa = 11.84 SYY682 pKa = 11.78 AEE684 pKa = 4.01 TVKK687 pKa = 10.66 RR688 pKa = 11.84 LYY690 pKa = 10.75 SDD692 pKa = 4.2 PLMKK696 pKa = 10.43 AQQKK700 pKa = 6.03 TRR702 pKa = 11.84 KK703 pKa = 8.91 SSAMKK708 pKa = 10.13 II709 pKa = 3.65
Molecular weight: 81.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.322
IPC2_protein 8.346
IPC_protein 8.214
Toseland 8.668
ProMoST 8.712
Dawson 9.092
Bjellqvist 9.019
Wikipedia 9.253
Rodwell 9.209
Grimsley 9.092
Solomon 9.136
Lehninger 9.092
Nozaki 9.048
DTASelect 8.902
Thurlkill 8.99
EMBOSS 9.209
Sillero 9.165
Patrickios 4.393
IPC_peptide 9.121
IPC2_peptide 7.702
IPC2.peptide.svr19 7.664
Protein with the highest isoelectric point:
>tr|A0A1L3KIB4|A0A1L3KIB4_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 24 OX=1922955 PE=4 SV=1
MM1 pKa = 8.13 DD2 pKa = 3.55 NTITHH7 pKa = 7.21 ALCYY11 pKa = 9.64 AANITLGKK19 pKa = 9.63 DD20 pKa = 2.94 AKK22 pKa = 10.55 PFIEE26 pKa = 5.11 RR27 pKa = 11.84 IEE29 pKa = 4.45 HH30 pKa = 5.7 FLSHH34 pKa = 6.87 NGTEE38 pKa = 4.24 YY39 pKa = 10.37 TVNRR43 pKa = 11.84 LKK45 pKa = 10.9 VIYY48 pKa = 10.05 QGALLLRR55 pKa = 11.84 EE56 pKa = 4.12 YY57 pKa = 10.51 DD58 pKa = 3.47 QLGRR62 pKa = 11.84 PVDD65 pKa = 3.64 TDD67 pKa = 3.64 FLGNTNRR74 pKa = 11.84 GSFDD78 pKa = 3.42 QAVKK82 pKa = 10.4 LYY84 pKa = 9.92 HH85 pKa = 6.63 EE86 pKa = 4.85 NGIATSKK93 pKa = 10.46 KK94 pKa = 10.25 GYY96 pKa = 9.24 PKK98 pKa = 10.51 DD99 pKa = 3.29 STGIVVRR106 pKa = 11.84 QFVEE110 pKa = 4.25 SNRR113 pKa = 11.84 PQVIRR118 pKa = 11.84 NMTGLLRR125 pKa = 11.84 SYY127 pKa = 9.11 TQFRR131 pKa = 11.84 LDD133 pKa = 3.75 SVSQQQVDD141 pKa = 3.74 KK142 pKa = 10.87 VVEE145 pKa = 4.56 SVCMPRR151 pKa = 11.84 DD152 pKa = 3.32 RR153 pKa = 11.84 AHH155 pKa = 7.77 DD156 pKa = 3.87 EE157 pKa = 3.92 NQLFDD162 pKa = 4.22 NLIHH166 pKa = 7.35 LSRR169 pKa = 11.84 VPKK172 pKa = 10.51 ALWNKK177 pKa = 9.46 SIKK180 pKa = 10.37 AEE182 pKa = 4.04 RR183 pKa = 11.84 LSMDD187 pKa = 3.78 CLRR190 pKa = 11.84 PYY192 pKa = 10.09 TSVYY196 pKa = 10.41 CHH198 pKa = 6.54 GSVPKK203 pKa = 10.15 HH204 pKa = 6.25 VKK206 pKa = 10.29 DD207 pKa = 3.73 EE208 pKa = 4.6 PYY210 pKa = 11.12 GKK212 pKa = 10.11 ALYY215 pKa = 11.24 SMITTSYY222 pKa = 10.37 LPSPLEE228 pKa = 3.81 SVVPSKK234 pKa = 10.77 SIRR237 pKa = 11.84 DD238 pKa = 3.73 RR239 pKa = 11.84 VRR241 pKa = 11.84 SWMPRR246 pKa = 11.84 EE247 pKa = 3.86 NDD249 pKa = 3.38 YY250 pKa = 11.59 DD251 pKa = 3.76 PEE253 pKa = 4.1 QYY255 pKa = 10.42 AGKK258 pKa = 9.5 IHH260 pKa = 7.37 IIQEE264 pKa = 4.07 AGAKK268 pKa = 9.44 ARR270 pKa = 11.84 TVAIPNGWTQIGFVPLHH287 pKa = 6.78 DD288 pKa = 3.84 QMQLEE293 pKa = 4.47 SEE295 pKa = 4.56 RR296 pKa = 11.84 YY297 pKa = 8.48 FPSEE301 pKa = 3.82 SCVFDD306 pKa = 3.59 QLKK309 pKa = 10.67 GIYY312 pKa = 10.0 ALRR315 pKa = 11.84 NHH317 pKa = 6.4 MGDD320 pKa = 2.55 GKK322 pKa = 9.21 TPVSVDD328 pKa = 3.47 LSSATDD334 pKa = 3.6 RR335 pKa = 11.84 FPIEE339 pKa = 4.25 FQCKK343 pKa = 8.47 LLSHH347 pKa = 7.12 IGLPFYY353 pKa = 10.94 SDD355 pKa = 3.51 SLSEE359 pKa = 4.05 LVKK362 pKa = 10.9 KK363 pKa = 10.07 PWEE366 pKa = 4.31 FPQGKK371 pKa = 8.37 PFGYY375 pKa = 10.66 DD376 pKa = 2.66 SVYY379 pKa = 11.09 YY380 pKa = 10.54 NAGQPMGLYY389 pKa = 10.38 GSFPTFHH396 pKa = 7.21 FSNLVMADD404 pKa = 3.75 ASCRR408 pKa = 11.84 LTDD411 pKa = 3.64 YY412 pKa = 11.18 QIGEE416 pKa = 4.43 TIDD419 pKa = 3.83 GKK421 pKa = 11.2 GKK423 pKa = 9.31 EE424 pKa = 4.16 QVAGIVYY431 pKa = 8.65 EE432 pKa = 4.03 RR433 pKa = 11.84 LYY435 pKa = 11.24 GFYY438 pKa = 10.75 AHH440 pKa = 7.66 RR441 pKa = 11.84 DD442 pKa = 3.32 KK443 pKa = 11.34 DD444 pKa = 4.33 TVPDD448 pKa = 4.73 DD449 pKa = 4.04 YY450 pKa = 11.45 IEE452 pKa = 4.47 RR453 pKa = 11.84 NNILGNAAGDD463 pKa = 4.01 SPVSKK468 pKa = 10.26 FYY470 pKa = 10.97 DD471 pKa = 3.5 GSRR474 pKa = 11.84 FKK476 pKa = 11.36 VLGDD480 pKa = 4.48 DD481 pKa = 3.96 IVFSDD486 pKa = 4.05 EE487 pKa = 4.18 RR488 pKa = 11.84 DD489 pKa = 3.24 AAHH492 pKa = 5.73 YY493 pKa = 7.92 TKK495 pKa = 10.39 IMKK498 pKa = 8.63 TINVPISHH506 pKa = 6.87 HH507 pKa = 6.26 KK508 pKa = 10.31 SFSKK512 pKa = 10.9 GMVCEE517 pKa = 4.15 FAGAIAMRR525 pKa = 11.84 TKK527 pKa = 10.62 KK528 pKa = 10.46 SNKK531 pKa = 8.4 DD532 pKa = 2.88 RR533 pKa = 11.84 RR534 pKa = 11.84 YY535 pKa = 9.77 DD536 pKa = 3.37 VSIFRR541 pKa = 11.84 PYY543 pKa = 10.76 KK544 pKa = 9.47 FPKK547 pKa = 10.14 DD548 pKa = 3.26 GSFIGNPISFIYY560 pKa = 10.35 AFSSPQSKK568 pKa = 9.76 QRR570 pKa = 11.84 VSKK573 pKa = 9.95 KK574 pKa = 7.69 WKK576 pKa = 9.99 QFFEE580 pKa = 5.47 DD581 pKa = 3.79 FDD583 pKa = 3.53 KK584 pKa = 11.1 TRR586 pKa = 11.84 PQRR589 pKa = 11.84 YY590 pKa = 9.4 ADD592 pKa = 4.31 LCPITPFVEE601 pKa = 4.0 DD602 pKa = 2.75 RR603 pKa = 11.84 VGIVGRR609 pKa = 11.84 FRR611 pKa = 11.84 DD612 pKa = 3.54 QDD614 pKa = 2.83 IDD616 pKa = 3.78 RR617 pKa = 11.84 VVDD620 pKa = 3.99 NIYY623 pKa = 10.63 RR624 pKa = 11.84 FSNLSFFPYY633 pKa = 10.19 TDD635 pKa = 3.22 TGKK638 pKa = 8.81 DD639 pKa = 3.13 TPIIYY644 pKa = 9.87 DD645 pKa = 3.7 GPRR648 pKa = 11.84 NRR650 pKa = 11.84 APLYY654 pKa = 9.31 QVPLFIKK661 pKa = 10.26 KK662 pKa = 10.13 KK663 pKa = 8.82 GIEE666 pKa = 4.03 VPGFEE671 pKa = 4.43 TQRR674 pKa = 11.84 PASDD678 pKa = 2.92 TRR680 pKa = 11.84 SYY682 pKa = 11.78 AEE684 pKa = 4.01 TVKK687 pKa = 10.66 RR688 pKa = 11.84 LYY690 pKa = 10.75 SDD692 pKa = 4.2 PLMKK696 pKa = 10.43 AQQKK700 pKa = 6.03 TRR702 pKa = 11.84 KK703 pKa = 8.91 SSAMKK708 pKa = 10.13 II709 pKa = 3.65
Molecular weight: 81.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.322
IPC2_protein 8.346
IPC_protein 8.214
Toseland 8.668
ProMoST 8.712
Dawson 9.092
Bjellqvist 9.019
Wikipedia 9.253
Rodwell 9.209
Grimsley 9.092
Solomon 9.136
Lehninger 9.092
Nozaki 9.048
DTASelect 8.902
Thurlkill 8.99
EMBOSS 9.209
Sillero 9.165
Patrickios 4.393
IPC_peptide 9.121
IPC2_peptide 7.702
IPC2.peptide.svr19 7.664
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
709
709
709
709.0
81.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.219 ± 0.0
1.269 ± 0.0
7.475 ± 0.0
4.372 ± 0.0
5.501 ± 0.0
6.206 ± 0.0
2.398 ± 0.0
6.065 ± 0.0
7.193 ± 0.0
6.206 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.116 ± 0.0
3.667 ± 0.0
6.065 ± 0.0
4.231 ± 0.0
6.629 ± 0.0
8.039 ± 0.0
4.795 ± 0.0
6.488 ± 0.0
0.705 ± 0.0
5.36 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here