Romboutsia weinsteinii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Romboutsia

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3979 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A371J5N4|A0A371J5N4_9FIRM 2 3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Romboutsia weinsteinii OX=2020949 GN=gpmA PE=3 SV=1
MM1 pKa = 7.68LGNGMYY7 pKa = 9.94QVGIDD12 pKa = 4.58LPAGDD17 pKa = 4.33YY18 pKa = 10.52FVLKK22 pKa = 11.01DD23 pKa = 3.32EE24 pKa = 4.49DD25 pKa = 4.63AYY27 pKa = 10.25MGSYY31 pKa = 10.42KK32 pKa = 9.03VTKK35 pKa = 10.16DD36 pKa = 3.18LSNDD40 pKa = 3.33YY41 pKa = 11.14GSTLLSDD48 pKa = 4.11AFTNFDD54 pKa = 3.8YY55 pKa = 11.1FSVEE59 pKa = 3.77DD60 pKa = 3.8GNYY63 pKa = 10.08VKK65 pKa = 11.09LEE67 pKa = 4.28DD68 pKa = 3.62CTIYY72 pKa = 10.47PKK74 pKa = 11.12NEE76 pKa = 3.76VEE78 pKa = 5.77LDD80 pKa = 3.7FLDD83 pKa = 5.95AEE85 pKa = 4.73LLTNGTFEE93 pKa = 4.61VGVDD97 pKa = 4.57LPAGDD102 pKa = 4.32YY103 pKa = 10.79KK104 pKa = 11.23LEE106 pKa = 4.68SEE108 pKa = 4.79DD109 pKa = 2.62GWYY112 pKa = 9.24TIRR115 pKa = 11.84EE116 pKa = 4.63GIGANYY122 pKa = 9.96ILITADD128 pKa = 3.05TFKK131 pKa = 11.2NFTYY135 pKa = 10.73VQLQDD140 pKa = 3.47GYY142 pKa = 10.72FIRR145 pKa = 11.84LDD147 pKa = 3.98DD148 pKa = 3.99KK149 pKa = 11.45TSLILNN155 pKa = 4.5

Molecular weight: 17.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A371IYP6|A0A371IYP6_9FIRM Threonine--tRNA ligase OS=Romboutsia weinsteinii OX=2020949 GN=thrS PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 9.11TSNGRR28 pKa = 11.84NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84NRR41 pKa = 11.84LTHH44 pKa = 6.18

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3979

0

3979

1204381

27

2744

302.7

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.361 ± 0.045

1.196 ± 0.017

5.903 ± 0.038

7.254 ± 0.051

4.213 ± 0.029

6.331 ± 0.044

1.368 ± 0.016

10.26 ± 0.059

8.63 ± 0.046

9.002 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.719 ± 0.021

6.455 ± 0.038

2.667 ± 0.026

2.28 ± 0.019

3.114 ± 0.026

6.722 ± 0.034

4.99 ± 0.036

6.664 ± 0.04

0.643 ± 0.013

4.227 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski