Staphylococcus phage Maine

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Kayvirus; unclassified Kayvirus

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1K706|A0A5C1K706_9CAUD Uncharacterized protein OS=Staphylococcus phage Maine OX=2589661 GN=CPT_Maine_001 PE=4 SV=1
MM1 pKa = 8.6IEE3 pKa = 3.94IRR5 pKa = 11.84LDD7 pKa = 3.41NNYY10 pKa = 10.46SDD12 pKa = 5.81FNLKK16 pKa = 10.17VLLKK20 pKa = 9.97RR21 pKa = 11.84IKK23 pKa = 10.13RR24 pKa = 11.84VAPRR28 pKa = 11.84EE29 pKa = 3.87LTYY32 pKa = 10.72GIEE35 pKa = 4.26SVEE38 pKa = 4.54DD39 pKa = 3.07IDD41 pKa = 5.67VNVEE45 pKa = 3.86PVNPYY50 pKa = 10.38SDD52 pKa = 4.25YY53 pKa = 9.89EE54 pKa = 4.29HH55 pKa = 7.29LSEE58 pKa = 5.54VYY60 pKa = 10.78VSDD63 pKa = 4.0LFIVITLNDD72 pKa = 3.78FVAYY76 pKa = 9.38YY77 pKa = 10.0QGEE80 pKa = 4.47RR81 pKa = 11.84YY82 pKa = 10.05SGEE85 pKa = 3.96SLDD88 pKa = 5.25EE89 pKa = 5.11IIHH92 pKa = 7.18DD93 pKa = 4.24MFYY96 pKa = 11.36DD97 pKa = 4.03DD98 pKa = 6.23ADD100 pKa = 4.26PFDD103 pKa = 5.36LDD105 pKa = 3.86YY106 pKa = 11.77

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1K5X3|A0A5C1K5X3_9CAUD Uncharacterized protein OS=Staphylococcus phage Maine OX=2589661 GN=CPT_Maine_094 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.3SSKK5 pKa = 10.92GLVNLMYY12 pKa = 10.21EE13 pKa = 4.76RR14 pKa = 11.84YY15 pKa = 9.81RR16 pKa = 11.84ILPGRR21 pKa = 11.84GFVSDD26 pKa = 2.99RR27 pKa = 11.84RR28 pKa = 11.84LRR30 pKa = 11.84MVVSLHH36 pKa = 6.08SVSNTKK42 pKa = 10.35RR43 pKa = 11.84LVYY46 pKa = 10.16KK47 pKa = 9.72YY48 pKa = 9.49IVHH51 pKa = 6.68MYY53 pKa = 9.24FF54 pKa = 3.74

Molecular weight: 6.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

219

0

219

42812

33

1351

195.5

22.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.193 ± 0.193

0.491 ± 0.052

6.93 ± 0.133

8.383 ± 0.226

3.994 ± 0.121

5.779 ± 0.295

1.588 ± 0.08

7.505 ± 0.193

9.14 ± 0.243

8.147 ± 0.161

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.113

6.531 ± 0.154

2.768 ± 0.141

3.55 ± 0.171

3.583 ± 0.094

6.547 ± 0.159

5.921 ± 0.178

6.428 ± 0.185

0.855 ± 0.064

5.108 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski