Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIMB 8712)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Beijerinckiaceae; Beijerinckia; Beijerinckia indica; Beijerinckia indica subsp. indica

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3774 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|B2IL60|B2IL60_BEII9 Ribonuclease VapC OS=Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIMB 8712) OX=395963 GN=vapC PE=3 SV=1
MM1 pKa = 7.82LDD3 pKa = 3.43ATTLAQLQNIANAQTNVGVKK23 pKa = 9.88NAVVNIINLATGSNPASSTLAQTVNTLLGGPNGPMSAKK61 pKa = 10.59ANGSALFYY69 pKa = 10.96SDD71 pKa = 5.68DD72 pKa = 4.3DD73 pKa = 4.49NNPGKK78 pKa = 9.89PNLQAAKK85 pKa = 10.27DD86 pKa = 4.01CQTSLKK92 pKa = 10.38GNGYY96 pKa = 9.75FLADD100 pKa = 3.66TQAGVAAEE108 pKa = 4.09KK109 pKa = 10.4LANQADD115 pKa = 4.22ALSGAVNTSPAGDD128 pKa = 4.43AIMGLASAMMGAQADD143 pKa = 3.6NAYY146 pKa = 10.49AFVSNAYY153 pKa = 9.95SGSIWTQYY161 pKa = 10.2EE162 pKa = 4.14LPALLGQTGSEE173 pKa = 3.96VGTVNGLNLSNYY185 pKa = 9.38NNYY188 pKa = 10.11SPSQIQNLISASQACDD204 pKa = 3.67PNNLPPPTGGVPANDD219 pKa = 3.66NLSTTQMIALGLLGAATTVGVGALIILGAPVEE251 pKa = 4.37IPALGVGGIMLLLTTSQAAAKK272 pKa = 9.3PIAPTTTDD280 pKa = 3.31AASQYY285 pKa = 9.8QFQWQLNTYY294 pKa = 9.26NGAQIAPALGYY305 pKa = 10.18ILAQAQGAPNVSSLAMQVQPSLVSLRR331 pKa = 11.84NLVTQSNSPLFAQTTDD347 pKa = 2.78SSGNLVNVTIQSNSSNSLLIQLASATATTAVNPATIDD384 pKa = 3.3VGFNNSGGTSFEE396 pKa = 4.01QTTVTNANGSAAVLISGDD414 pKa = 4.22DD415 pKa = 3.71NNASINNSTISLNSGATATITGNSNTFALGSDD447 pKa = 3.46SSVTLNSQGGSNTVTVTGDD466 pKa = 3.03PWFSWLASQTTRR478 pKa = 11.84FEE480 pKa = 4.04ADD482 pKa = 2.96ANGVRR487 pKa = 11.84VGYY490 pKa = 9.6PGGNLGTDD498 pKa = 3.43YY499 pKa = 11.39SLNDD503 pKa = 3.29QGQITAAPVFMGFGLGGGYY522 pKa = 10.74ASDD525 pKa = 3.71SAEE528 pKa = 4.1AGSLQSSVSGTIPADD543 pKa = 3.66TSSGPPLDD551 pKa = 3.79AWLSSVVVINLGGGVGSGTLYY572 pKa = 9.0TAPSGFIQGGRR583 pKa = 11.84VLSSTDD589 pKa = 3.1MAVIYY594 pKa = 10.2PNANNDD600 pKa = 3.37GVFNNSPSGNVAGYY614 pKa = 9.8NRR616 pKa = 11.84SANDD620 pKa = 3.23QLSAPSQNLQDD631 pKa = 4.11LVGGTVSALQTLSSVSPLFGAALADD656 pKa = 3.69MASNASSWINTDD668 pKa = 3.39PLVIGTTSAGITLTNWISNAVYY690 pKa = 10.38FDD692 pKa = 4.1TNVNPTTLQADD703 pKa = 4.41GKK705 pKa = 9.52LHH707 pKa = 5.26HH708 pKa = 6.85TSWVSGGTGIVALDD722 pKa = 3.66RR723 pKa = 11.84NGNGQIDD730 pKa = 5.59DD731 pKa = 3.64ITEE734 pKa = 4.04TLSEE738 pKa = 4.37FFQGGSNPGHH748 pKa = 6.07YY749 pKa = 10.39ADD751 pKa = 5.64GLDD754 pKa = 3.7ALKK757 pKa = 10.95SLAQAGASVFSAATSLTDD775 pKa = 3.42PTTHH779 pKa = 5.84QSYY782 pKa = 8.74WSEE785 pKa = 3.69LRR787 pKa = 11.84VWLDD791 pKa = 3.22ANQNGVTDD799 pKa = 3.98AGEE802 pKa = 4.3LKK804 pKa = 9.76TLNDD808 pKa = 4.52LGITSINLVGSGNLGEE824 pKa = 4.4SLEE827 pKa = 4.48GSAVTNRR834 pKa = 11.84TTYY837 pKa = 9.38TKK839 pKa = 10.85SDD841 pKa = 3.41GSSGAVAAVDD851 pKa = 4.41FATNTVGDD859 pKa = 3.92IATSANGGITIQSVPEE875 pKa = 4.36GGATPSNSFVIKK887 pKa = 9.31NTSAHH892 pKa = 7.07AYY894 pKa = 7.16TLSNGQLIDD903 pKa = 3.49NTTGQTVIASGTKK916 pKa = 10.27GVFSTNQNDD925 pKa = 4.24SIAVASSDD933 pKa = 3.55PTPYY937 pKa = 10.11WLGGGTGAATLTGGAGNTVFLINSKK962 pKa = 9.46TIVHH966 pKa = 6.96GGTGFNIAEE975 pKa = 4.26VTDD978 pKa = 4.2AKK980 pKa = 10.8PITVDD985 pKa = 3.87LKK987 pKa = 9.71TANLQEE993 pKa = 4.49VIGGAGDD1000 pKa = 3.97GVFNASGTNWNVFIQGGSGNNIIIGGTANDD1030 pKa = 4.01ALAGGTGDD1038 pKa = 4.86DD1039 pKa = 4.85LIEE1042 pKa = 4.89AGTGGSVIHH1051 pKa = 6.95AGAGNDD1057 pKa = 3.61VIYY1060 pKa = 10.34GGSGSTSTAQPAYY1073 pKa = 10.65VNAGNTADD1081 pKa = 3.12ISYY1084 pKa = 10.75VEE1086 pKa = 4.13RR1087 pKa = 11.84LYY1089 pKa = 11.09EE1090 pKa = 4.25GGFGRR1095 pKa = 11.84EE1096 pKa = 3.75ASAGEE1101 pKa = 4.09LQWCVSVLGNGSVDD1115 pKa = 3.35RR1116 pKa = 11.84VTLASYY1122 pKa = 10.02FLSNPEE1128 pKa = 3.49WQNRR1132 pKa = 11.84FGTQTDD1138 pKa = 3.56AQFVTSVFDD1147 pKa = 3.51NFLGRR1152 pKa = 11.84DD1153 pKa = 3.63PSAGEE1158 pKa = 3.78LAQFTQALADD1168 pKa = 3.9GAPRR1172 pKa = 11.84SYY1174 pKa = 11.27ALEE1177 pKa = 4.13SVADD1181 pKa = 3.79NAQSQTYY1188 pKa = 8.59WGLHH1192 pKa = 5.92HH1193 pKa = 7.4PGASDD1198 pKa = 3.58VIYY1201 pKa = 10.41GGSGHH1206 pKa = 6.1DD1207 pKa = 3.8TIVLGTNNAVVYY1219 pKa = 10.16AGSGTMTVVGNANGFSTVGLHH1240 pKa = 7.08GSYY1243 pKa = 11.26ADD1245 pKa = 3.45YY1246 pKa = 10.67TLTHH1250 pKa = 6.42NADD1253 pKa = 3.21GTITVTNIGHH1263 pKa = 6.85EE1264 pKa = 4.63DD1265 pKa = 3.65GDD1267 pKa = 4.17GTVTMKK1273 pKa = 10.83NVTALDD1279 pKa = 3.84FKK1281 pKa = 11.55DD1282 pKa = 5.47FIQVDD1287 pKa = 3.25IATALGMPVDD1297 pKa = 4.12DD1298 pKa = 4.99TLNTGNASQVTVNASGQYY1316 pKa = 9.38VISAITLLANDD1327 pKa = 4.82LDD1329 pKa = 4.44YY1330 pKa = 11.48AGKK1333 pKa = 10.0SLSIRR1338 pKa = 11.84EE1339 pKa = 4.04LLDD1342 pKa = 3.48NNGNAIARR1350 pKa = 11.84GSSGQVNGGIAMLSTDD1366 pKa = 3.36GSSITFTPTPGFAGVMSFRR1385 pKa = 11.84YY1386 pKa = 9.36HH1387 pKa = 8.21VEE1389 pKa = 4.1DD1390 pKa = 4.04SDD1392 pKa = 4.08GQTGQSVVQTGTSNTAEE1409 pKa = 4.08LAATVSLVTPSQPTDD1424 pKa = 3.33PLFAKK1429 pKa = 10.16EE1430 pKa = 4.24WFLQAADD1437 pKa = 4.22VIPLLKK1443 pKa = 9.98HH1444 pKa = 3.76YY1445 pKa = 8.77TGAGVSVGIFDD1456 pKa = 4.14GSGNVDD1462 pKa = 4.21FSNPDD1467 pKa = 3.35LSAHH1471 pKa = 6.98AGQSVKK1477 pKa = 10.77LDD1479 pKa = 3.56GTPGITQTGTHH1490 pKa = 5.95ATLIAGVLGAAIDD1503 pKa = 3.97GEE1505 pKa = 4.76GAVGVAPDD1513 pKa = 3.38ATLNSEE1519 pKa = 4.19AVGLVGMSTQTDD1531 pKa = 3.89ANLMDD1536 pKa = 3.91WSNYY1540 pKa = 9.96DD1541 pKa = 3.18VVNNSFSVKK1550 pKa = 10.05PPFYY1554 pKa = 10.97NLGLSPAGSASEE1566 pKa = 3.85LTAFLNAIQNGRR1578 pKa = 11.84QGYY1581 pKa = 7.36GTIIVMGGGNSRR1593 pKa = 11.84AQGEE1597 pKa = 4.45NTNDD1601 pKa = 3.24SYY1603 pKa = 11.3LTSSPYY1609 pKa = 10.66AITVGGINATTDD1621 pKa = 3.28LSTLRR1626 pKa = 11.84ISGAPFSDD1634 pKa = 3.17PGYY1637 pKa = 10.02SILISAPANNITSDD1651 pKa = 3.14GVAYY1655 pKa = 9.69TNAYY1659 pKa = 8.18GQNFGSTVKK1668 pKa = 9.49TAEE1671 pKa = 4.21GTSFATPIVSGVVADD1686 pKa = 3.97MLQANPYY1693 pKa = 9.97LSWKK1697 pKa = 10.23DD1698 pKa = 3.41VQQILAYY1705 pKa = 10.06SAVKK1709 pKa = 10.33VDD1711 pKa = 4.58PNDD1714 pKa = 3.66TNPFVAGSTAGSGWAFNGATNWNGGGLHH1742 pKa = 6.14YY1743 pKa = 11.14SPDD1746 pKa = 3.6YY1747 pKa = 11.52GFGEE1751 pKa = 4.09VDD1753 pKa = 2.75ARR1755 pKa = 11.84AAVRR1759 pKa = 11.84LAQSWSSFGIYY1770 pKa = 9.69TDD1772 pKa = 3.74VFSHH1776 pKa = 6.28DD1777 pKa = 3.89TYY1779 pKa = 11.51VISTPLSSGPFSKK1792 pKa = 9.67TFTEE1796 pKa = 4.68SSTTDD1801 pKa = 3.06TVEE1804 pKa = 4.2TVQVRR1809 pKa = 11.84VKK1811 pKa = 10.01ISNAIRR1817 pKa = 11.84SNLTITLISPSGVTSTLMARR1837 pKa = 11.84PGSVIGPIADD1847 pKa = 3.46AANFVPGAFDD1857 pKa = 4.91DD1858 pKa = 4.39FTPGTFDD1865 pKa = 3.35YY1866 pKa = 11.32TFMTQADD1873 pKa = 3.99RR1874 pKa = 11.84GEE1876 pKa = 4.18AFQGNWTVNISDD1888 pKa = 4.07AAGSTSQAIVTDD1900 pKa = 3.4VKK1902 pKa = 10.82IILQGNYY1909 pKa = 7.63TAGKK1913 pKa = 7.84PQTFIYY1919 pKa = 9.73TDD1921 pKa = 3.43EE1922 pKa = 4.34FATLSSTPGNALYY1935 pKa = 10.58DD1936 pKa = 4.0PNNAARR1942 pKa = 11.84FTLTGSGYY1950 pKa = 10.33QDD1952 pKa = 3.61TLNVSATTGAVNLDD1966 pKa = 4.14LVTGSTDD1973 pKa = 2.98STIDD1977 pKa = 3.27GRR1979 pKa = 11.84YY1980 pKa = 8.94LAIAPGSKK1988 pKa = 9.49INKK1991 pKa = 8.77VYY1993 pKa = 10.89LGDD1996 pKa = 3.93GVSTVHH2002 pKa = 6.83LNADD2006 pKa = 3.13NDD2008 pKa = 4.33IINTGAGNAILYY2020 pKa = 9.83GGAGFDD2026 pKa = 3.98IYY2028 pKa = 11.29NIGKK2032 pKa = 8.32WLQRR2036 pKa = 11.84SIPAGNTTIYY2046 pKa = 10.39GGSGGSAVYY2055 pKa = 10.28IGDD2058 pKa = 3.65EE2059 pKa = 4.18AGSNILVGGAGVDD2072 pKa = 3.64TVSYY2076 pKa = 10.14SSASAGVTVNLGVTTAQATRR2096 pKa = 11.84STGTDD2101 pKa = 2.87TLLGFEE2107 pKa = 4.74NLTGSAYY2114 pKa = 11.0NDD2116 pKa = 3.24VLTGNGGNNVIDD2128 pKa = 4.11GGAGNDD2134 pKa = 4.34TIDD2137 pKa = 4.24GGPGNDD2143 pKa = 3.71TLIGGAGIDD2152 pKa = 3.69TVSYY2156 pKa = 11.1ASATSGVTVSLALTTAQATGGAGTDD2181 pKa = 3.44TLSGFEE2187 pKa = 4.34NLTGSAYY2194 pKa = 10.96NDD2196 pKa = 3.52VLTGDD2201 pKa = 3.99SGDD2204 pKa = 3.68NVIDD2208 pKa = 3.91GGAGNDD2214 pKa = 4.65LIDD2217 pKa = 4.61GGLGNDD2223 pKa = 3.93TLIGGAGIDD2232 pKa = 3.57TLTYY2236 pKa = 10.7ASATSGVTVSLALTTAQATGGAGTDD2261 pKa = 3.44TLSGFEE2267 pKa = 4.34NLTGSAYY2274 pKa = 10.95NDD2276 pKa = 3.38VLTGSGGDD2284 pKa = 3.55NVIDD2288 pKa = 3.92GGAGNDD2294 pKa = 4.65LIDD2297 pKa = 4.61GGLGNDD2303 pKa = 3.93TLIGGAGVDD2312 pKa = 3.38TVTYY2316 pKa = 10.26VSATSGVTVSLALTTAQATGGAGTDD2341 pKa = 3.44TLSGFEE2347 pKa = 4.34NLTGSAYY2354 pKa = 10.88SDD2356 pKa = 3.56VLTGDD2361 pKa = 3.54GGDD2364 pKa = 3.52NVIDD2368 pKa = 4.0GGAGNDD2374 pKa = 3.66TLYY2377 pKa = 11.29GGAGNDD2383 pKa = 3.77TLIGGAGNDD2392 pKa = 3.92KK2393 pKa = 10.72LQGGAGDD2400 pKa = 3.99DD2401 pKa = 4.06TLDD2404 pKa = 3.92GGAGTDD2410 pKa = 3.24AASYY2414 pKa = 7.99TQALAGVTVSLAVSGPQNTIGAGTDD2439 pKa = 3.09TLLNIEE2445 pKa = 4.63NLTGSIYY2452 pKa = 10.91DD2453 pKa = 3.98DD2454 pKa = 4.2VLTGDD2459 pKa = 4.04AGNNVIDD2466 pKa = 4.34GNAGNDD2472 pKa = 4.25LIDD2475 pKa = 4.43GGLGNDD2481 pKa = 3.75TLIGGSGTDD2490 pKa = 3.3TVSYY2494 pKa = 11.14ASATSGVTVSLALTTAQNTGGAGTDD2519 pKa = 3.39TLSGFEE2525 pKa = 4.34NLTGSAYY2532 pKa = 10.96NDD2534 pKa = 3.52VLTGDD2539 pKa = 3.88SGNNVIDD2546 pKa = 4.17GGLGNDD2552 pKa = 4.11TLDD2555 pKa = 3.74GGAGVDD2561 pKa = 3.36TVTYY2565 pKa = 10.26VSATSGVTVSLALTTAQATGGAGTDD2590 pKa = 3.44TLSGFEE2596 pKa = 4.34NLTGSAYY2603 pKa = 10.88SDD2605 pKa = 3.56VLTGDD2610 pKa = 3.54GGDD2613 pKa = 3.52NVIDD2617 pKa = 4.0GGAGNDD2623 pKa = 3.66TLYY2626 pKa = 11.29GGAGNDD2632 pKa = 3.77TLIGGAGNDD2641 pKa = 3.92KK2642 pKa = 10.72LQGGAGDD2649 pKa = 3.99DD2650 pKa = 4.06TLDD2653 pKa = 3.92GGAGTDD2659 pKa = 3.24AASYY2663 pKa = 7.99TQALAGVTVSLAVSGPQNTIGAGTDD2688 pKa = 3.09TLLNIEE2694 pKa = 4.63NLTGSIYY2701 pKa = 10.91DD2702 pKa = 3.98DD2703 pKa = 4.2VLTGDD2708 pKa = 4.04AGNNVIDD2715 pKa = 4.34GNAGNDD2721 pKa = 4.25LIDD2724 pKa = 4.43GGLGNDD2730 pKa = 3.76TLIGGTGIDD2739 pKa = 3.33TVTYY2743 pKa = 8.86TSATSGVTVSLALTTAQNTGGAGTDD2768 pKa = 3.39TLSGFEE2774 pKa = 4.34NLTGSAYY2781 pKa = 10.96NDD2783 pKa = 3.74VLTGDD2788 pKa = 3.93AGNNVINGGAGNDD2801 pKa = 3.48ILVGSAGSDD2810 pKa = 3.46TLDD2813 pKa = 3.45GGAGNDD2819 pKa = 3.23VYY2821 pKa = 10.61IYY2823 pKa = 10.39HH2824 pKa = 6.1QADD2827 pKa = 3.78GNMLIHH2833 pKa = 7.55DD2834 pKa = 5.2AGDD2837 pKa = 3.68SSGPTPFNTLSFADD2851 pKa = 3.83LTLSNLLFSQSGTDD2865 pKa = 3.58LVIGIAGTGHH2875 pKa = 7.04SITVQNEE2882 pKa = 3.87LGASGLDD2889 pKa = 4.0GIQQINFADD2898 pKa = 4.58GSNMTHH2904 pKa = 5.94AQILAHH2910 pKa = 6.61AA2911 pKa = 4.95

Molecular weight:
291.12 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|B2IDV4|B2IDV4_BEII9 Ribonuclease P protein component OS=Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIMB 8712) OX=395963 GN=rnpA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 8.8IVRR12 pKa = 11.84KK13 pKa = 9.24RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.55GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.05VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight:
5.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3774

0

3774

1205051

41

2911

319.3

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.719 ± 0.051

0.888 ± 0.012

5.443 ± 0.031

5.694 ± 0.039

4.0 ± 0.026

8.027 ± 0.036

2.317 ± 0.021

5.711 ± 0.028

3.641 ± 0.031

10.636 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.018

2.823 ± 0.032

5.385 ± 0.036

3.395 ± 0.02

6.595 ± 0.04

5.713 ± 0.033

5.463 ± 0.029

6.728 ± 0.032

1.271 ± 0.015

2.332 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski