Bombilactobacillus mellis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Bombilactobacillus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F4KQ95|A0A0F4KQ95_9LACO Putative transcriptional regulator lytR family OS=Bombilactobacillus mellis OX=1218508 GN=JG29_15200 PE=3 SV=1
MM1 pKa = 7.23TKK3 pKa = 10.1IKK5 pKa = 10.13IVYY8 pKa = 10.51ASMTGNDD15 pKa = 3.63EE16 pKa = 4.95DD17 pKa = 3.99IADD20 pKa = 3.84ILSEE24 pKa = 4.2DD25 pKa = 3.82FEE27 pKa = 4.58EE28 pKa = 6.64AGMQVDD34 pKa = 4.64LSEE37 pKa = 4.6ISQTDD42 pKa = 3.47VADD45 pKa = 3.63YY46 pKa = 10.6LQADD50 pKa = 3.89VCIMVSYY57 pKa = 9.54TYY59 pKa = 11.24SDD61 pKa = 3.43GLEE64 pKa = 4.01GNIPDD69 pKa = 4.15EE70 pKa = 4.6GLDD73 pKa = 3.81FYY75 pKa = 11.61HH76 pKa = 7.71DD77 pKa = 4.33LLEE80 pKa = 5.56SDD82 pKa = 4.91LSNKK86 pKa = 9.34IFGVCGSGDD95 pKa = 3.15TFYY98 pKa = 10.95PEE100 pKa = 3.65FCVAVDD106 pKa = 3.57QFEE109 pKa = 4.38KK110 pKa = 11.19AFLQAHH116 pKa = 6.57ARR118 pKa = 11.84QGAKK122 pKa = 9.4SVKK125 pKa = 9.88IEE127 pKa = 4.05LAPNNDD133 pKa = 4.7DD134 pKa = 4.36IVKK137 pKa = 10.01LDD139 pKa = 4.21HH140 pKa = 6.55FAQTIVEE147 pKa = 4.2NCQQ150 pKa = 2.86

Molecular weight: 16.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F4KYU7|A0A0F4KYU7_9LACO L-ribulose-5-phosphate 4-epimerase OS=Bombilactobacillus mellis OX=1218508 GN=JG29_01680 PE=4 SV=1
MM1 pKa = 6.82TKK3 pKa = 10.26KK4 pKa = 9.84RR5 pKa = 11.84LKK7 pKa = 10.19VISIILIIVAFLLVFAGYY25 pKa = 9.39RR26 pKa = 11.84QQTAALVPTRR36 pKa = 11.84LHH38 pKa = 5.3STPTLFLHH46 pKa = 6.8GWGSSINAEE55 pKa = 4.05KK56 pKa = 10.87QMTSAAQRR64 pKa = 11.84AGVTNSVTQALVDD77 pKa = 3.63RR78 pKa = 11.84QGRR81 pKa = 11.84VHH83 pKa = 7.14LKK85 pKa = 10.22GHH87 pKa = 6.84IPPHH91 pKa = 5.68ARR93 pKa = 11.84NPIIEE98 pKa = 4.34VGFKK102 pKa = 10.8DD103 pKa = 3.63NRR105 pKa = 11.84NPNYY109 pKa = 10.15HH110 pKa = 6.6QDD112 pKa = 3.69GRR114 pKa = 11.84WLKK117 pKa = 10.32QVILKK122 pKa = 9.03LQQQYY127 pKa = 10.03QIKK130 pKa = 9.66NINLVGHH137 pKa = 6.07SMGNMAISYY146 pKa = 9.71YY147 pKa = 11.14LLDD150 pKa = 3.84NADD153 pKa = 3.6NQRR156 pKa = 11.84LPKK159 pKa = 10.03LRR161 pKa = 11.84KK162 pKa = 7.8QVAIAGHH169 pKa = 5.63FAGIIGAGDD178 pKa = 3.45RR179 pKa = 11.84PHH181 pKa = 7.27RR182 pKa = 11.84LHH184 pKa = 6.97LAATGRR190 pKa = 11.84PNHH193 pKa = 6.35MDD195 pKa = 3.07NNYY198 pKa = 7.25RR199 pKa = 11.84TLMGLRR205 pKa = 11.84QRR207 pKa = 11.84YY208 pKa = 7.93PNKK211 pKa = 9.46QVKK214 pKa = 9.05VLNIFGDD221 pKa = 4.0KK222 pKa = 10.89NDD224 pKa = 4.05GTNSDD229 pKa = 3.7GDD231 pKa = 3.86VTNASAQSLRR241 pKa = 11.84YY242 pKa = 9.15LVQARR247 pKa = 11.84ALSYY251 pKa = 9.09QEE253 pKa = 3.99RR254 pKa = 11.84KK255 pKa = 9.63IVGSDD260 pKa = 3.41GQHH263 pKa = 5.44SRR265 pKa = 11.84LHH267 pKa = 5.44EE268 pKa = 4.17SRR270 pKa = 11.84RR271 pKa = 11.84VDD273 pKa = 3.76RR274 pKa = 11.84ILINFLWKK282 pKa = 10.52

Molecular weight: 31.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1572

0

1572

510670

38

1594

324.9

36.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.681 ± 0.066

0.622 ± 0.015

5.172 ± 0.053

4.568 ± 0.059

4.253 ± 0.047

6.146 ± 0.06

2.258 ± 0.028

7.824 ± 0.057

6.322 ± 0.053

10.446 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.338 ± 0.031

5.49 ± 0.072

3.771 ± 0.035

6.505 ± 0.082

3.652 ± 0.048

5.886 ± 0.056

5.8 ± 0.054

6.448 ± 0.05

1.084 ± 0.023

3.735 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski