Sphingobium faniae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4753 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G4SFY6|A0A1G4SFY6_9SPHN Glycosyltransferase involved in cell wall bisynthesis OS=Sphingobium faniae OX=570446 GN=SAMN02927924_02008 PE=4 SV=1
MM1 pKa = 7.38RR2 pKa = 11.84TTYY5 pKa = 11.22DD6 pKa = 3.12SATVRR11 pKa = 11.84LYY13 pKa = 10.77HH14 pKa = 7.17LSDD17 pKa = 3.54AQEE20 pKa = 4.34GGAATTLFYY29 pKa = 11.3GSLNEE34 pKa = 4.18ALRR37 pKa = 11.84IAAGQSADD45 pKa = 3.65VQDD48 pKa = 4.39GLFLATDD55 pKa = 3.84NDD57 pKa = 3.68VVAYY61 pKa = 10.71LDD63 pKa = 5.55LIDD66 pKa = 5.17DD67 pKa = 4.16

Molecular weight: 7.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G4RG10|A0A1G4RG10_9SPHN N-acetylmuramoyl-L-alanine amidase OS=Sphingobium faniae OX=570446 GN=SAMN02927924_01317 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4753

0

4753

1446485

39

4587

304.3

33.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.88 ± 0.051

0.837 ± 0.012

6.04 ± 0.028

5.537 ± 0.034

3.51 ± 0.019

8.815 ± 0.051

2.103 ± 0.02

5.243 ± 0.025

3.043 ± 0.027

9.802 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.019

2.571 ± 0.025

5.237 ± 0.031

3.272 ± 0.021

7.507 ± 0.049

5.347 ± 0.035

5.063 ± 0.041

6.911 ± 0.03

1.407 ± 0.014

2.218 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski