Lake Sarah-associated circular molecule 11
Average proteome isoelectric point is 7.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A126G9B9|A0A126G9B9_9VIRU Putative coat protein OS=Lake Sarah-associated circular molecule 11 OX=1685726 PE=4 SV=1
MM1 pKa = 7.36 AVEE4 pKa = 4.13 DD5 pKa = 4.15 KK6 pKa = 10.65 KK7 pKa = 11.21 RR8 pKa = 11.84 KK9 pKa = 9.62 LGVVSTGAVPGGKK22 pKa = 8.04 TYY24 pKa = 10.73 VPRR27 pKa = 11.84 GISNRR32 pKa = 11.84 PEE34 pKa = 4.18 LKK36 pKa = 10.74 HH37 pKa = 6.53 FDD39 pKa = 3.61 LTVGVAALDD48 pKa = 3.41 TWQMVTNTILCSIRR62 pKa = 11.84 LGTGPTQRR70 pKa = 11.84 IGRR73 pKa = 11.84 KK74 pKa = 8.55 IRR76 pKa = 11.84 VKK78 pKa = 10.22 SVVFRR83 pKa = 11.84 GRR85 pKa = 11.84 MEE87 pKa = 4.71 LGVAANNANSAYY99 pKa = 9.43 TIDD102 pKa = 5.18 FIWDD106 pKa = 3.31 KK107 pKa = 9.4 QTNGSTPTMGTIYY120 pKa = 9.93 TGSTAYY126 pKa = 10.69 SLPNPDD132 pKa = 2.91 NDD134 pKa = 5.13 EE135 pKa = 3.76 RR136 pKa = 11.84 FEE138 pKa = 4.17 WVKK141 pKa = 10.63 RR142 pKa = 11.84 ISRR145 pKa = 11.84 DD146 pKa = 3.46 DD147 pKa = 3.61 PASNFTLVNTKK158 pKa = 9.57 FNCNKK163 pKa = 10.14 LISYY167 pKa = 10.47 DD168 pKa = 3.56 SDD170 pKa = 3.76 LGTVSDD176 pKa = 4.17 LTQNNLLMTMCSPFDD191 pKa = 3.68 ATPTLAGEE199 pKa = 4.0 LRR201 pKa = 11.84 VYY203 pKa = 10.57 FVDD206 pKa = 3.98 EE207 pKa = 4.15
Molecular weight: 22.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.68
IPC2_protein 7.892
IPC_protein 7.951
Toseland 7.907
ProMoST 8.287
Dawson 8.58
Bjellqvist 8.756
Wikipedia 8.565
Rodwell 8.609
Grimsley 7.629
Solomon 8.829
Lehninger 8.829
Nozaki 8.916
DTASelect 8.565
Thurlkill 8.639
EMBOSS 8.799
Sillero 8.916
Patrickios 4.622
IPC_peptide 8.814
IPC2_peptide 7.644
IPC2.peptide.svr19 7.732
Protein with the highest isoelectric point:
>tr|A0A126G9B9|A0A126G9B9_9VIRU Putative coat protein OS=Lake Sarah-associated circular molecule 11 OX=1685726 PE=4 SV=1
MM1 pKa = 7.36 AVEE4 pKa = 4.13 DD5 pKa = 4.15 KK6 pKa = 10.65 KK7 pKa = 11.21 RR8 pKa = 11.84 KK9 pKa = 9.62 LGVVSTGAVPGGKK22 pKa = 8.04 TYY24 pKa = 10.73 VPRR27 pKa = 11.84 GISNRR32 pKa = 11.84 PEE34 pKa = 4.18 LKK36 pKa = 10.74 HH37 pKa = 6.53 FDD39 pKa = 3.61 LTVGVAALDD48 pKa = 3.41 TWQMVTNTILCSIRR62 pKa = 11.84 LGTGPTQRR70 pKa = 11.84 IGRR73 pKa = 11.84 KK74 pKa = 8.55 IRR76 pKa = 11.84 VKK78 pKa = 10.22 SVVFRR83 pKa = 11.84 GRR85 pKa = 11.84 MEE87 pKa = 4.71 LGVAANNANSAYY99 pKa = 9.43 TIDD102 pKa = 5.18 FIWDD106 pKa = 3.31 KK107 pKa = 9.4 QTNGSTPTMGTIYY120 pKa = 9.93 TGSTAYY126 pKa = 10.69 SLPNPDD132 pKa = 2.91 NDD134 pKa = 5.13 EE135 pKa = 3.76 RR136 pKa = 11.84 FEE138 pKa = 4.17 WVKK141 pKa = 10.63 RR142 pKa = 11.84 ISRR145 pKa = 11.84 DD146 pKa = 3.46 DD147 pKa = 3.61 PASNFTLVNTKK158 pKa = 9.57 FNCNKK163 pKa = 10.14 LISYY167 pKa = 10.47 DD168 pKa = 3.56 SDD170 pKa = 3.76 LGTVSDD176 pKa = 4.17 LTQNNLLMTMCSPFDD191 pKa = 3.68 ATPTLAGEE199 pKa = 4.0 LRR201 pKa = 11.84 VYY203 pKa = 10.57 FVDD206 pKa = 3.98 EE207 pKa = 4.15
Molecular weight: 22.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.68
IPC2_protein 7.892
IPC_protein 7.951
Toseland 7.907
ProMoST 8.287
Dawson 8.58
Bjellqvist 8.756
Wikipedia 8.565
Rodwell 8.609
Grimsley 7.629
Solomon 8.829
Lehninger 8.829
Nozaki 8.916
DTASelect 8.565
Thurlkill 8.639
EMBOSS 8.799
Sillero 8.916
Patrickios 4.622
IPC_peptide 8.814
IPC2_peptide 7.644
IPC2.peptide.svr19 7.732
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
207
207
207
207.0
22.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.797 ± 0.0
1.449 ± 0.0
6.763 ± 0.0
3.382 ± 0.0
3.865 ± 0.0
7.729 ± 0.0
0.483 ± 0.0
4.831 ± 0.0
5.314 ± 0.0
7.729 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.899 ± 0.0
6.763 ± 0.0
4.831 ± 0.0
1.932 ± 0.0
6.28 ± 0.0
6.763 ± 0.0
10.628 ± 0.0
8.213 ± 0.0
1.449 ± 0.0
2.899 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here