Amino acid dipepetide frequency for Microbacterium phage Noelani

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.751AlaAla: 21.751 ± 3.367
0.744AlaCys: 0.744 ± 0.351
9.295AlaAsp: 9.295 ± 1.341
7.808AlaGlu: 7.808 ± 1.411
4.09AlaPhe: 4.09 ± 0.686
18.219AlaGly: 18.219 ± 1.969
1.115AlaHis: 1.115 ± 0.509
5.391AlaIle: 5.391 ± 1.093
5.02AlaLys: 5.02 ± 0.954
13.014AlaLeu: 13.014 ± 1.698
3.718AlaMet: 3.718 ± 1.032
2.045AlaAsn: 2.045 ± 0.569
7.622AlaPro: 7.622 ± 1.156
4.09AlaGln: 4.09 ± 0.811
7.622AlaArg: 7.622 ± 1.064
5.763AlaSer: 5.763 ± 0.762
5.205AlaThr: 5.205 ± 1.106
8.924AlaVal: 8.924 ± 1.892
2.045AlaTrp: 2.045 ± 0.597
2.975AlaTyr: 2.975 ± 0.726
0.0AlaXaa: 0.0 ± 0.0
Cys
0.186CysAla: 0.186 ± 0.211
0.186CysCys: 0.186 ± 0.225
0.186CysAsp: 0.186 ± 0.17
0.558CysGlu: 0.558 ± 0.378
0.186CysPhe: 0.186 ± 0.17
0.186CysGly: 0.186 ± 0.225
0.0CysHis: 0.0 ± 0.0
0.0CysIle: 0.0 ± 0.0
0.186CysLys: 0.186 ± 0.162
0.186CysLeu: 0.186 ± 0.218
0.186CysMet: 0.186 ± 0.21
0.372CysAsn: 0.372 ± 0.248
0.0CysPro: 0.0 ± 0.0
0.186CysGln: 0.186 ± 0.225
0.558CysArg: 0.558 ± 0.351
0.186CysSer: 0.186 ± 0.225
0.186CysThr: 0.186 ± 0.225
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.372CysTyr: 0.372 ± 0.252
0.0CysXaa: 0.0 ± 0.0
Asp
10.411AspAla: 10.411 ± 1.9
0.186AspCys: 0.186 ± 0.218
2.975AspAsp: 2.975 ± 0.861
4.09AspGlu: 4.09 ± 1.248
0.744AspPhe: 0.744 ± 0.461
5.02AspGly: 5.02 ± 1.085
0.93AspHis: 0.93 ± 0.433
0.744AspIle: 0.744 ± 0.326
0.186AspLys: 0.186 ± 0.233
7.065AspLeu: 7.065 ± 1.322
0.744AspMet: 0.744 ± 0.54
1.673AspAsn: 1.673 ± 0.528
2.789AspPro: 2.789 ± 0.738
1.487AspGln: 1.487 ± 0.615
3.16AspArg: 3.16 ± 0.916
2.603AspSer: 2.603 ± 0.573
3.532AspThr: 3.532 ± 0.778
5.02AspVal: 5.02 ± 1.039
1.115AspTrp: 1.115 ± 0.409
1.487AspTyr: 1.487 ± 0.45
0.0AspXaa: 0.0 ± 0.0
Glu
6.879GluAla: 6.879 ± 1.01
0.186GluCys: 0.186 ± 0.225
2.975GluAsp: 2.975 ± 0.562
0.558GluGlu: 0.558 ± 0.42
0.744GluPhe: 0.744 ± 0.319
3.346GluGly: 3.346 ± 0.769
0.372GluHis: 0.372 ± 0.266
3.532GluIle: 3.532 ± 0.763
0.744GluLys: 0.744 ± 0.366
9.295GluLeu: 9.295 ± 1.736
0.372GluMet: 0.372 ± 0.26
3.718GluAsn: 3.718 ± 0.86
2.045GluPro: 2.045 ± 0.874
3.346GluGln: 3.346 ± 0.66
4.834GluArg: 4.834 ± 1.398
3.16GluSer: 3.16 ± 0.626
4.09GluThr: 4.09 ± 1.086
2.231GluVal: 2.231 ± 0.474
1.301GluTrp: 1.301 ± 0.505
1.673GluTyr: 1.673 ± 0.516
0.0GluXaa: 0.0 ± 0.0
Phe
3.904PheAla: 3.904 ± 1.207
0.0PheCys: 0.0 ± 0.0
2.603PheAsp: 2.603 ± 0.575
1.673PheGlu: 1.673 ± 0.758
0.186PhePhe: 0.186 ± 0.161
4.09PheGly: 4.09 ± 0.862
0.372PheHis: 0.372 ± 0.288
0.744PheIle: 0.744 ± 0.313
0.558PheLys: 0.558 ± 0.203
2.603PheLeu: 2.603 ± 0.672
0.744PheMet: 0.744 ± 0.309
1.673PheAsn: 1.673 ± 0.523
1.115PhePro: 1.115 ± 0.484
0.93PheGln: 0.93 ± 0.511
1.673PheArg: 1.673 ± 0.659
0.93PheSer: 0.93 ± 0.466
3.346PheThr: 3.346 ± 0.891
2.417PheVal: 2.417 ± 0.631
0.186PheTrp: 0.186 ± 0.176
0.558PheTyr: 0.558 ± 0.382
0.0PheXaa: 0.0 ± 0.0
Gly
11.34GlyAla: 11.34 ± 2.732
0.186GlyCys: 0.186 ± 0.162
5.02GlyAsp: 5.02 ± 1.197
3.904GlyGlu: 3.904 ± 0.88
3.346GlyPhe: 3.346 ± 0.41
6.135GlyGly: 6.135 ± 1.046
1.115GlyHis: 1.115 ± 0.45
5.02GlyIle: 5.02 ± 0.888
2.789GlyLys: 2.789 ± 0.541
7.808GlyLeu: 7.808 ± 2.182
1.859GlyMet: 1.859 ± 0.437
2.975GlyAsn: 2.975 ± 0.865
2.603GlyPro: 2.603 ± 1.247
3.16GlyGln: 3.16 ± 0.729
7.065GlyArg: 7.065 ± 0.946
7.25GlySer: 7.25 ± 0.959
5.391GlyThr: 5.391 ± 1.062
9.481GlyVal: 9.481 ± 1.261
2.231GlyTrp: 2.231 ± 0.819
1.859GlyTyr: 1.859 ± 0.508
0.0GlyXaa: 0.0 ± 0.0
His
2.231HisAla: 2.231 ± 0.714
0.0HisCys: 0.0 ± 0.0
1.301HisAsp: 1.301 ± 0.451
0.0HisGlu: 0.0 ± 0.0
0.558HisPhe: 0.558 ± 0.357
1.301HisGly: 1.301 ± 0.688
0.0HisHis: 0.0 ± 0.0
0.186HisIle: 0.186 ± 0.17
0.558HisLys: 0.558 ± 0.305
1.301HisLeu: 1.301 ± 0.561
0.186HisMet: 0.186 ± 0.161
0.558HisAsn: 0.558 ± 0.333
1.115HisPro: 1.115 ± 0.392
0.0HisGln: 0.0 ± 0.0
0.93HisArg: 0.93 ± 0.426
0.744HisSer: 0.744 ± 0.365
0.744HisThr: 0.744 ± 0.349
2.045HisVal: 2.045 ± 0.592
0.372HisTrp: 0.372 ± 0.22
0.744HisTyr: 0.744 ± 0.346
0.0HisXaa: 0.0 ± 0.0
Ile
4.462IleAla: 4.462 ± 0.98
0.0IleCys: 0.0 ± 0.0
3.904IleAsp: 3.904 ± 0.701
5.577IleGlu: 5.577 ± 0.808
0.558IlePhe: 0.558 ± 0.268
4.276IleGly: 4.276 ± 1.01
0.93IleHis: 0.93 ± 0.456
0.93IleIle: 0.93 ± 0.468
0.744IleLys: 0.744 ± 0.264
3.346IleLeu: 3.346 ± 1.309
0.186IleMet: 0.186 ± 0.233
0.558IleAsn: 0.558 ± 0.297
3.346IlePro: 3.346 ± 1.021
1.115IleGln: 1.115 ± 0.412
3.532IleArg: 3.532 ± 0.764
3.904IleSer: 3.904 ± 0.713
2.789IleThr: 2.789 ± 0.683
3.904IleVal: 3.904 ± 0.739
0.372IleTrp: 0.372 ± 0.214
0.558IleTyr: 0.558 ± 0.356
0.0IleXaa: 0.0 ± 0.0
Lys
2.045LysAla: 2.045 ± 0.453
0.0LysCys: 0.0 ± 0.0
1.487LysAsp: 1.487 ± 0.541
0.558LysGlu: 0.558 ± 0.323
0.744LysPhe: 0.744 ± 0.508
1.301LysGly: 1.301 ± 0.463
0.186LysHis: 0.186 ± 0.167
1.487LysIle: 1.487 ± 0.466
0.558LysLys: 0.558 ± 0.351
2.231LysLeu: 2.231 ± 0.629
0.372LysMet: 0.372 ± 0.253
1.115LysAsn: 1.115 ± 0.673
0.93LysPro: 0.93 ± 0.428
0.744LysGln: 0.744 ± 0.337
1.859LysArg: 1.859 ± 0.842
1.487LysSer: 1.487 ± 0.369
2.231LysThr: 2.231 ± 0.656
0.558LysVal: 0.558 ± 0.414
0.372LysTrp: 0.372 ± 0.224
0.558LysTyr: 0.558 ± 0.285
0.0LysXaa: 0.0 ± 0.0
Leu
9.667LeuAla: 9.667 ± 0.999
0.744LeuCys: 0.744 ± 0.405
5.949LeuAsp: 5.949 ± 1.255
2.045LeuGlu: 2.045 ± 0.707
4.09LeuPhe: 4.09 ± 0.894
7.808LeuGly: 7.808 ± 0.933
1.673LeuHis: 1.673 ± 0.582
4.462LeuIle: 4.462 ± 1.302
1.487LeuLys: 1.487 ± 0.726
7.436LeuLeu: 7.436 ± 2.134
2.789LeuMet: 2.789 ± 0.675
3.718LeuAsn: 3.718 ± 0.979
8.18LeuPro: 8.18 ± 1.747
6.135LeuGln: 6.135 ± 1.05
5.763LeuArg: 5.763 ± 0.957
5.577LeuSer: 5.577 ± 1.099
7.436LeuThr: 7.436 ± 1.022
5.949LeuVal: 5.949 ± 1.351
0.558LeuTrp: 0.558 ± 0.263
1.859LeuTyr: 1.859 ± 0.646
0.0LeuXaa: 0.0 ± 0.0
Met
2.975MetAla: 2.975 ± 0.55
0.0MetCys: 0.0 ± 0.0
1.487MetAsp: 1.487 ± 0.475
0.93MetGlu: 0.93 ± 0.356
0.744MetPhe: 0.744 ± 0.311
2.231MetGly: 2.231 ± 0.683
0.372MetHis: 0.372 ± 0.266
1.487MetIle: 1.487 ± 0.778
0.186MetLys: 0.186 ± 0.17
0.558MetLeu: 0.558 ± 0.326
0.0MetMet: 0.0 ± 0.0
0.93MetAsn: 0.93 ± 0.404
1.487MetPro: 1.487 ± 0.476
0.372MetGln: 0.372 ± 0.311
1.487MetArg: 1.487 ± 0.617
2.789MetSer: 2.789 ± 1.249
1.487MetThr: 1.487 ± 0.562
1.487MetVal: 1.487 ± 0.477
0.186MetTrp: 0.186 ± 0.17
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
6.693AsnAla: 6.693 ± 0.966
0.186AsnCys: 0.186 ± 0.17
0.372AsnAsp: 0.372 ± 0.224
1.115AsnGlu: 1.115 ± 0.343
0.558AsnPhe: 0.558 ± 0.246
4.648AsnGly: 4.648 ± 0.891
0.558AsnHis: 0.558 ± 0.263
0.558AsnIle: 0.558 ± 0.357
0.744AsnLys: 0.744 ± 0.34
2.417AsnLeu: 2.417 ± 0.75
0.744AsnMet: 0.744 ± 0.364
1.115AsnAsn: 1.115 ± 0.414
1.301AsnPro: 1.301 ± 0.444
0.0AsnGln: 0.0 ± 0.0
2.603AsnArg: 2.603 ± 0.605
2.045AsnSer: 2.045 ± 0.46
1.673AsnThr: 1.673 ± 0.511
2.789AsnVal: 2.789 ± 0.74
0.186AsnTrp: 0.186 ± 0.177
0.558AsnTyr: 0.558 ± 0.29
0.0AsnXaa: 0.0 ± 0.0
Pro
8.924ProAla: 8.924 ± 1.794
0.372ProCys: 0.372 ± 0.313
2.603ProAsp: 2.603 ± 0.856
2.603ProGlu: 2.603 ± 0.835
1.115ProPhe: 1.115 ± 0.453
4.276ProGly: 4.276 ± 0.808
0.93ProHis: 0.93 ± 0.444
2.045ProIle: 2.045 ± 0.545
1.301ProLys: 1.301 ± 0.473
4.648ProLeu: 4.648 ± 1.139
0.744ProMet: 0.744 ± 0.371
2.045ProAsn: 2.045 ± 0.606
1.487ProPro: 1.487 ± 0.813
2.417ProGln: 2.417 ± 0.629
2.789ProArg: 2.789 ± 0.752
3.346ProSer: 3.346 ± 0.372
4.09ProThr: 4.09 ± 0.86
4.276ProVal: 4.276 ± 0.731
0.558ProTrp: 0.558 ± 0.304
1.487ProTyr: 1.487 ± 0.476
0.0ProXaa: 0.0 ± 0.0
Gln
4.462GlnAla: 4.462 ± 0.949
0.0GlnCys: 0.0 ± 0.0
1.301GlnAsp: 1.301 ± 0.435
1.301GlnGlu: 1.301 ± 0.41
2.045GlnPhe: 2.045 ± 0.459
2.975GlnGly: 2.975 ± 0.882
1.301GlnHis: 1.301 ± 0.459
1.859GlnIle: 1.859 ± 0.678
0.558GlnLys: 0.558 ± 0.323
7.808GlnLeu: 7.808 ± 1.444
0.93GlnMet: 0.93 ± 0.488
0.93GlnAsn: 0.93 ± 0.302
2.975GlnPro: 2.975 ± 0.983
2.789GlnGln: 2.789 ± 0.699
3.346GlnArg: 3.346 ± 0.84
2.417GlnSer: 2.417 ± 0.602
1.487GlnThr: 1.487 ± 0.505
1.487GlnVal: 1.487 ± 0.514
0.186GlnTrp: 0.186 ± 0.156
0.744GlnTyr: 0.744 ± 0.357
0.0GlnXaa: 0.0 ± 0.0
Arg
9.109ArgAla: 9.109 ± 1.776
0.372ArgCys: 0.372 ± 0.252
5.205ArgAsp: 5.205 ± 0.893
5.949ArgGlu: 5.949 ± 1.458
2.603ArgPhe: 2.603 ± 0.693
4.462ArgGly: 4.462 ± 0.87
0.93ArgHis: 0.93 ± 0.484
3.532ArgIle: 3.532 ± 0.735
1.115ArgLys: 1.115 ± 0.382
6.879ArgLeu: 6.879 ± 1.186
1.115ArgMet: 1.115 ± 0.419
1.673ArgAsn: 1.673 ± 0.422
2.045ArgPro: 2.045 ± 0.645
3.904ArgGln: 3.904 ± 0.794
8.552ArgArg: 8.552 ± 2.119
2.789ArgSer: 2.789 ± 0.583
2.789ArgThr: 2.789 ± 0.644
5.577ArgVal: 5.577 ± 0.891
1.859ArgTrp: 1.859 ± 0.604
2.417ArgTyr: 2.417 ± 0.523
0.0ArgXaa: 0.0 ± 0.0
Ser
7.994SerAla: 7.994 ± 1.166
0.186SerCys: 0.186 ± 0.225
2.231SerAsp: 2.231 ± 0.517
4.462SerGlu: 4.462 ± 0.88
0.93SerPhe: 0.93 ± 0.294
4.834SerGly: 4.834 ± 0.822
0.93SerHis: 0.93 ± 0.364
4.276SerIle: 4.276 ± 0.652
1.301SerLys: 1.301 ± 0.552
3.532SerLeu: 3.532 ± 0.647
2.231SerMet: 2.231 ± 0.757
1.487SerAsn: 1.487 ± 0.677
1.673SerPro: 1.673 ± 0.384
2.789SerGln: 2.789 ± 0.622
3.346SerArg: 3.346 ± 0.728
4.648SerSer: 4.648 ± 0.907
4.834SerThr: 4.834 ± 0.962
3.718SerVal: 3.718 ± 0.687
1.301SerTrp: 1.301 ± 0.425
1.673SerTyr: 1.673 ± 0.564
0.0SerXaa: 0.0 ± 0.0
Thr
9.853ThrAla: 9.853 ± 1.55
0.0ThrCys: 0.0 ± 0.0
2.975ThrAsp: 2.975 ± 0.783
4.09ThrGlu: 4.09 ± 1.035
3.904ThrPhe: 3.904 ± 0.882
5.763ThrGly: 5.763 ± 1.101
1.115ThrHis: 1.115 ± 0.529
3.346ThrIle: 3.346 ± 1.213
0.93ThrLys: 0.93 ± 0.401
5.205ThrLeu: 5.205 ± 1.048
1.673ThrMet: 1.673 ± 0.657
1.487ThrAsn: 1.487 ± 0.54
5.205ThrPro: 5.205 ± 0.952
1.487ThrGln: 1.487 ± 0.539
4.276ThrArg: 4.276 ± 0.989
1.859ThrSer: 1.859 ± 0.526
3.904ThrThr: 3.904 ± 0.904
5.763ThrVal: 5.763 ± 0.987
1.115ThrTrp: 1.115 ± 0.467
0.744ThrTyr: 0.744 ± 0.5
0.0ThrXaa: 0.0 ± 0.0
Val
10.039ValAla: 10.039 ± 1.134
0.0ValCys: 0.0 ± 0.0
2.603ValAsp: 2.603 ± 0.785
5.763ValGlu: 5.763 ± 1.312
1.301ValPhe: 1.301 ± 0.656
5.391ValGly: 5.391 ± 1.193
0.744ValHis: 0.744 ± 0.364
3.904ValIle: 3.904 ± 1.034
1.301ValLys: 1.301 ± 0.515
4.648ValLeu: 4.648 ± 1.035
1.487ValMet: 1.487 ± 0.768
2.045ValAsn: 2.045 ± 0.841
4.09ValPro: 4.09 ± 0.778
4.276ValGln: 4.276 ± 1.445
6.135ValArg: 6.135 ± 1.331
5.577ValSer: 5.577 ± 0.928
7.065ValThr: 7.065 ± 1.479
5.577ValVal: 5.577 ± 1.172
0.93ValTrp: 0.93 ± 0.414
1.301ValTyr: 1.301 ± 0.34
0.0ValXaa: 0.0 ± 0.0
Trp
1.673TrpAla: 1.673 ± 0.512
0.0TrpCys: 0.0 ± 0.0
0.558TrpAsp: 0.558 ± 0.312
0.744TrpGlu: 0.744 ± 0.405
1.301TrpPhe: 1.301 ± 0.391
0.744TrpGly: 0.744 ± 0.255
0.93TrpHis: 0.93 ± 0.42
1.115TrpIle: 1.115 ± 0.393
0.0TrpLys: 0.0 ± 0.0
2.603TrpLeu: 2.603 ± 0.715
0.186TrpMet: 0.186 ± 0.218
0.372TrpAsn: 0.372 ± 0.22
0.372TrpPro: 0.372 ± 0.34
0.372TrpGln: 0.372 ± 0.273
0.744TrpArg: 0.744 ± 0.345
0.744TrpSer: 0.744 ± 0.329
1.859TrpThr: 1.859 ± 0.635
0.186TrpVal: 0.186 ± 0.225
0.0TrpTrp: 0.0 ± 0.0
0.744TrpTyr: 0.744 ± 0.404
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.045TyrAla: 2.045 ± 0.534
0.558TyrCys: 0.558 ± 0.313
0.93TyrAsp: 0.93 ± 0.414
1.673TyrGlu: 1.673 ± 0.435
0.558TyrPhe: 0.558 ± 0.3
3.532TyrGly: 3.532 ± 0.773
0.372TyrHis: 0.372 ± 0.261
0.372TyrIle: 0.372 ± 0.321
0.558TyrLys: 0.558 ± 0.306
0.744TyrLeu: 0.744 ± 0.472
0.744TyrMet: 0.744 ± 0.346
0.372TyrAsn: 0.372 ± 0.263
1.673TyrPro: 1.673 ± 0.62
1.301TyrGln: 1.301 ± 0.364
2.603TyrArg: 2.603 ± 0.784
0.558TyrSer: 0.558 ± 0.351
0.558TyrThr: 0.558 ± 0.337
2.789TyrVal: 2.789 ± 0.725
0.372TyrTrp: 0.372 ± 0.22
0.744TyrTyr: 0.744 ± 0.385
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 25 proteins (5380 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski