Helicobacter anseris
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1577 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3D8J731|A0A3D8J731_9HELI Ribonuclease R OS=Helicobacter anseris OX=375926 GN=CQA57_05315 PE=4 SV=1
MM1 pKa = 7.43 AVKK4 pKa = 9.0 ITDD7 pKa = 3.0 ICIACGSCIDD17 pKa = 3.99 EE18 pKa = 5.04 CPVQAIVDD26 pKa = 4.12 DD27 pKa = 4.8 DD28 pKa = 4.88 DD29 pKa = 4.92 NPTGEE34 pKa = 4.53 GIYY37 pKa = 10.39 YY38 pKa = 10.05 VYY40 pKa = 10.8 SDD42 pKa = 3.91 KK43 pKa = 11.21 CVEE46 pKa = 4.37 CVGHH50 pKa = 6.77 NDD52 pKa = 3.68 APACASACPTDD63 pKa = 4.2 GCIVWSDD70 pKa = 3.01 AGSTKK75 pKa = 10.49 RR76 pKa = 11.84 EE77 pKa = 4.39 DD78 pKa = 2.89 IGADD82 pKa = 3.38 LRR84 pKa = 11.84 DD85 pKa = 3.46 GTTPVMM91 pKa = 4.4
Molecular weight: 9.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.858
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.503
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.719
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.151
Thurlkill 3.579
EMBOSS 3.732
Sillero 3.859
Patrickios 0.07
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|A0A3D8JAJ4|A0A3D8JAJ4_9HELI Chorismate synthase OS=Helicobacter anseris OX=375926 GN=aroC PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.31 RR14 pKa = 11.84 THH16 pKa = 5.88 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.07 TKK25 pKa = 10.39 NGRR28 pKa = 11.84 KK29 pKa = 9.3 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1577
0
1577
509887
26
3319
323.3
36.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.056 ± 0.054
1.182 ± 0.029
5.347 ± 0.043
6.21 ± 0.07
5.809 ± 0.069
6.108 ± 0.079
1.689 ± 0.029
9.384 ± 0.071
8.584 ± 0.073
9.923 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.241 ± 0.028
6.17 ± 0.096
2.835 ± 0.04
4.078 ± 0.04
3.151 ± 0.041
6.777 ± 0.061
4.895 ± 0.094
5.06 ± 0.057
0.712 ± 0.022
3.788 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here