Providencia phage vB PstP PS3
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A411AWH7|A0A411AWH7_9CAUD Uncharacterized protein OS=Providencia phage vB PstP PS3 OX=2508090 GN=Stuart_32 PE=4 SV=1
MM1 pKa = 7.58 VKK3 pKa = 9.27 FTFTNITLWAKK14 pKa = 10.14 MNGFQVGEE22 pKa = 4.19 SFDD25 pKa = 3.88 ALEE28 pKa = 4.61 LFEE31 pKa = 6.77 GDD33 pKa = 4.0 YY34 pKa = 11.2 DD35 pKa = 4.05 PSGLPTEE42 pKa = 5.31 SDD44 pKa = 3.79 DD45 pKa = 4.09 LPCVVFRR52 pKa = 11.84 RR53 pKa = 11.84 PSNGDD58 pKa = 3.36 LYY60 pKa = 10.8 ILSNCNEE67 pKa = 4.3 EE68 pKa = 4.91 GVLSCSS74 pKa = 3.49
Molecular weight: 8.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.78
IPC2_protein 3.961
IPC_protein 3.834
Toseland 3.643
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.668
Grimsley 3.567
Solomon 3.795
Lehninger 3.745
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.706
EMBOSS 3.757
Sillero 3.948
Patrickios 1.888
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|A0A411AWD9|A0A411AWD9_9CAUD Uncharacterized protein OS=Providencia phage vB PstP PS3 OX=2508090 GN=Stuart_2 PE=4 SV=1
MM1 pKa = 7.49 TKK3 pKa = 9.23 TEE5 pKa = 4.13 RR6 pKa = 11.84 KK7 pKa = 6.85 QLRR10 pKa = 11.84 RR11 pKa = 11.84 FGCTTKK17 pKa = 10.32 QAQEE21 pKa = 4.19 LEE23 pKa = 4.35 QNRR26 pKa = 11.84 QRR28 pKa = 11.84 LMQEE32 pKa = 3.75 ALTKK36 pKa = 10.34 EE37 pKa = 3.96 GSEE40 pKa = 3.58 ARR42 pKa = 11.84 KK43 pKa = 9.9 RR44 pKa = 11.84 IRR46 pKa = 11.84 QLMTVDD52 pKa = 3.62 CSPRR56 pKa = 11.84 VSAPKK61 pKa = 10.18 EE62 pKa = 3.83 YY63 pKa = 9.91 TCRR66 pKa = 11.84 AKK68 pKa = 10.43 QSRR71 pKa = 3.65
Molecular weight: 8.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.472
IPC_protein 9.823
Toseland 10.716
ProMoST 10.555
Dawson 10.774
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 11.096
Grimsley 10.789
Solomon 10.877
Lehninger 10.862
Nozaki 10.716
DTASelect 10.409
Thurlkill 10.687
EMBOSS 11.111
Sillero 10.701
Patrickios 10.862
IPC_peptide 10.891
IPC2_peptide 9.487
IPC2.peptide.svr19 8.641
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
52
0
52
12539
41
1269
241.1
26.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.486 ± 0.412
1.085 ± 0.201
6.013 ± 0.238
6.221 ± 0.282
3.365 ± 0.159
6.946 ± 0.297
1.962 ± 0.17
5.08 ± 0.218
6.213 ± 0.345
8.98 ± 0.283
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.775 ± 0.194
4.019 ± 0.194
3.621 ± 0.241
5.016 ± 0.327
4.825 ± 0.222
6.643 ± 0.329
6.54 ± 0.339
7.002 ± 0.343
1.172 ± 0.142
4.035 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here