Streptococcus phage Javan318

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AZ27|A0A4D6AZ27_9CAUD Tail assembly protein OS=Streptococcus phage Javan318 OX=2548105 GN=Javan318_0050 PE=4 SV=1
MM1 pKa = 7.52ALYY4 pKa = 10.33RR5 pKa = 11.84ATKK8 pKa = 10.48NIFFEE13 pKa = 4.24QLNKK17 pKa = 10.77DD18 pKa = 4.08VIVDD22 pKa = 4.34DD23 pKa = 5.44IIEE26 pKa = 4.32LDD28 pKa = 3.16EE29 pKa = 6.0DD30 pKa = 3.97YY31 pKa = 11.46AKK33 pKa = 10.68EE34 pKa = 4.11VNKK37 pKa = 10.32KK38 pKa = 10.01LKK40 pKa = 10.48NAFPDD45 pKa = 3.51VKK47 pKa = 10.82NVLEE51 pKa = 4.45LVDD54 pKa = 4.16KK55 pKa = 11.41NGTLEE60 pKa = 4.25LEE62 pKa = 4.08EE63 pKa = 5.13DD64 pKa = 3.97APSVDD69 pKa = 4.5DD70 pKa = 4.18VSQEE74 pKa = 4.23TVEE77 pKa = 4.28DD78 pKa = 3.71

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AY65|A0A4D6AY65_9CAUD Cytosine-specific methyltransferase OS=Streptococcus phage Javan318 OX=2548105 GN=Javan318_0015 PE=3 SV=1
MM1 pKa = 7.54SIAIKK6 pKa = 10.64VDD8 pKa = 3.49LQKK11 pKa = 11.11AKK13 pKa = 10.43HH14 pKa = 5.88KK15 pKa = 10.54LSSEE19 pKa = 3.71SMTRR23 pKa = 11.84GKK25 pKa = 10.46VAVASKK31 pKa = 10.55ILLDD35 pKa = 3.62NEE37 pKa = 4.16QYY39 pKa = 10.4IPLRR43 pKa = 11.84GGEE46 pKa = 4.01LRR48 pKa = 11.84ASGRR52 pKa = 11.84IVGQGDD58 pKa = 3.52AVVYY62 pKa = 7.1GTVYY66 pKa = 10.93ARR68 pKa = 11.84AQFYY72 pKa = 10.46GSNGIVTFRR81 pKa = 11.84RR82 pKa = 11.84YY83 pKa = 5.87TTPGTGKK90 pKa = 10.24RR91 pKa = 11.84WDD93 pKa = 3.52QVATSNHH100 pKa = 5.0AEE102 pKa = 3.47EE103 pKa = 4.19WARR106 pKa = 11.84AFVKK110 pKa = 10.83GMGLL114 pKa = 3.32

Molecular weight: 12.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11681

42

1093

208.6

23.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.157 ± 0.532

0.445 ± 0.081

6.224 ± 0.267

7.516 ± 0.391

4.109 ± 0.263

6.352 ± 0.339

1.507 ± 0.152

6.635 ± 0.255

8.15 ± 0.359

7.979 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.414 ± 0.135

6.172 ± 0.282

2.525 ± 0.2

4.255 ± 0.279

4.084 ± 0.249

6.386 ± 0.371

6.335 ± 0.348

6.395 ± 0.244

1.31 ± 0.214

4.049 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski