Escherichia virus EPS7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Epseptimavirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2I4F7|B2I4F7_9CAUD Uncharacterized protein OS=Escherichia virus EPS7 OX=491003 GN=AGC_0119 PE=4 SV=1
MM1 pKa = 8.03CDD3 pKa = 3.43LLVWPDD9 pKa = 3.63GSWCYY14 pKa = 10.37RR15 pKa = 11.84FEE17 pKa = 4.11FATDD21 pKa = 3.51DD22 pKa = 4.19FSWKK26 pKa = 10.3SDD28 pKa = 3.8DD29 pKa = 3.53FCVIYY34 pKa = 10.57EE35 pKa = 4.47DD36 pKa = 3.45TAEE39 pKa = 4.04WKK41 pKa = 10.46EE42 pKa = 3.71FLIKK46 pKa = 10.38EE47 pKa = 4.3GEE49 pKa = 4.15YY50 pKa = 11.2GEE52 pKa = 4.86EE53 pKa = 4.25DD54 pKa = 3.53FF55 pKa = 6.4

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2I4D8|B2I4D8_9CAUD Uncharacterized protein OS=Escherichia virus EPS7 OX=491003 GN=AGC_0100 PE=4 SV=1
MM1 pKa = 7.79AKK3 pKa = 9.77QKK5 pKa = 10.18NAKK8 pKa = 7.58TQAAPAVKK16 pKa = 9.57TFAQTEE22 pKa = 4.28ANRR25 pKa = 11.84KK26 pKa = 8.94ARR28 pKa = 11.84LEE30 pKa = 3.84RR31 pKa = 11.84HH32 pKa = 5.4LRR34 pKa = 11.84KK35 pKa = 10.0HH36 pKa = 5.61PTDD39 pKa = 3.63AQAQTALKK47 pKa = 9.66SAAPVRR53 pKa = 11.84QKK55 pKa = 10.88PKK57 pKa = 10.45AKK59 pKa = 10.24NSTRR63 pKa = 11.84ATAKK67 pKa = 9.62QVVFIKK73 pKa = 11.09GEE75 pKa = 3.99GHH77 pKa = 6.3KK78 pKa = 10.36SVPVTLSFNGGAEE91 pKa = 3.83MFARR95 pKa = 11.84NGMALKK101 pKa = 10.34DD102 pKa = 3.61YY103 pKa = 10.55EE104 pKa = 4.21KK105 pKa = 10.99AVNQKK110 pKa = 9.22RR111 pKa = 11.84KK112 pKa = 9.85PMADD116 pKa = 2.87VMRR119 pKa = 11.84EE120 pKa = 3.73SRR122 pKa = 11.84GQFGSVKK129 pKa = 9.73PNIFGVEE136 pKa = 3.77YY137 pKa = 10.96SRR139 pKa = 11.84DD140 pKa = 3.43NVRR143 pKa = 11.84ALCYY147 pKa = 10.52GIGIKK152 pKa = 9.02FTGASDD158 pKa = 3.58RR159 pKa = 11.84KK160 pKa = 9.51SAKK163 pKa = 7.84PTRR166 pKa = 11.84KK167 pKa = 9.61RR168 pKa = 11.84KK169 pKa = 9.86AKK171 pKa = 10.29

Molecular weight: 18.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

170

0

170

29196

34

949

171.7

19.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.789 ± 0.469

1.048 ± 0.097

6.36 ± 0.192

7.206 ± 0.204

4.189 ± 0.158

6.186 ± 0.194

1.898 ± 0.135

6.761 ± 0.182

7.206 ± 0.202

8.381 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.853 ± 0.118

5.028 ± 0.156

3.381 ± 0.149

3.699 ± 0.183

4.446 ± 0.143

6.155 ± 0.224

5.699 ± 0.194

6.528 ± 0.17

1.247 ± 0.085

3.939 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski