Human immunodeficiency virus type 1 group M subtype A (isolate MAL) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 unknown group

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P05955|VPR_HV1MA Protein Vpr OS=Human immunodeficiency virus type 1 group M subtype A (isolate MAL) OX=11697 GN=vpr PE=3 SV=1
QQ1 pKa = 7.35PLVILAIVALVVTLIIAIVVWTIVFIEE28 pKa = 3.68IRR30 pKa = 11.84KK31 pKa = 8.86IRR33 pKa = 11.84RR34 pKa = 11.84QRR36 pKa = 11.84KK37 pKa = 7.48IDD39 pKa = 3.6RR40 pKa = 11.84LIDD43 pKa = 4.43RR44 pKa = 11.84IRR46 pKa = 11.84EE47 pKa = 3.87RR48 pKa = 11.84AEE50 pKa = 3.95DD51 pKa = 3.58SGNEE55 pKa = 4.13SEE57 pKa = 5.74GDD59 pKa = 3.49TEE61 pKa = 4.21EE62 pKa = 4.23LSKK65 pKa = 10.81LVEE68 pKa = 4.41MGHH71 pKa = 6.47DD72 pKa = 3.98APWDD76 pKa = 3.67VDD78 pKa = 3.92DD79 pKa = 5.34LL80 pKa = 4.6

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P04603|NEF_HV1MA Protein Nef OS=Human immunodeficiency virus type 1 group M subtype A (isolate MAL) OX=11697 GN=nef PE=1 SV=3
MM1 pKa = 7.3EE2 pKa = 5.21NRR4 pKa = 11.84WQVMIVWQVDD14 pKa = 3.04RR15 pKa = 11.84MRR17 pKa = 11.84IRR19 pKa = 11.84TWHH22 pKa = 6.32SLVKK26 pKa = 10.03HH27 pKa = 5.86HH28 pKa = 6.71MYY30 pKa = 10.3VSKK33 pKa = 10.69KK34 pKa = 9.51AKK36 pKa = 8.13NWFYY40 pKa = 9.95RR41 pKa = 11.84HH42 pKa = 6.33HH43 pKa = 6.7YY44 pKa = 9.12EE45 pKa = 4.03SRR47 pKa = 11.84HH48 pKa = 5.45PKK50 pKa = 9.69VSSEE54 pKa = 3.72VHH56 pKa = 5.93IPLGDD61 pKa = 3.27ARR63 pKa = 11.84LVVRR67 pKa = 11.84TYY69 pKa = 10.17WGLQTGEE76 pKa = 4.41KK77 pKa = 9.7DD78 pKa = 2.67WHH80 pKa = 6.64LGHH83 pKa = 6.53GVSIEE88 pKa = 3.83WRR90 pKa = 11.84QKK92 pKa = 10.51RR93 pKa = 11.84YY94 pKa = 8.3STQLDD99 pKa = 3.69PDD101 pKa = 3.98LADD104 pKa = 3.82QLIHH108 pKa = 7.18LYY110 pKa = 10.94YY111 pKa = 10.17FDD113 pKa = 5.17CFSEE117 pKa = 4.36SAIRR121 pKa = 11.84QAILGHH127 pKa = 5.92IVSPRR132 pKa = 11.84CDD134 pKa = 3.35YY135 pKa = 10.96QAGHH139 pKa = 6.29NKK141 pKa = 9.61VGSLQYY147 pKa = 10.82LALTALIAPKK157 pKa = 8.96KK158 pKa = 8.26TRR160 pKa = 11.84PPLPSVRR167 pKa = 11.84KK168 pKa = 8.46LTEE171 pKa = 4.09DD172 pKa = 2.93RR173 pKa = 11.84WNKK176 pKa = 8.1PQQTKK181 pKa = 7.41GHH183 pKa = 6.58RR184 pKa = 11.84GSHH187 pKa = 4.81TMNGHH192 pKa = 6.05

Molecular weight: 22.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3657

72

1440

365.7

41.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.399 ± 0.697

2.106 ± 0.427

4.129 ± 0.274

6.891 ± 0.472

2.434 ± 0.171

7.41 ± 0.207

2.27 ± 0.743

6.946 ± 0.775

6.836 ± 1.081

8.094 ± 0.711

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.242

4.649 ± 0.805

5.551 ± 0.839

6.207 ± 0.522

6.016 ± 0.906

5.442 ± 0.514

5.66 ± 0.583

5.496 ± 0.477

2.652 ± 0.277

2.543 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski