Penicillium nordicum
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12938 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M8P025|A0A0M8P025_9EURO Uncharacterized protein OS=Penicillium nordicum OX=229535 GN=ACN38_g8358 PE=4 SV=1
MM1 pKa = 7.13 TFNVFLTGLLAATAAALPHH20 pKa = 5.95 SARR23 pKa = 11.84 SGEE26 pKa = 4.15 SGAVEE31 pKa = 4.25 TRR33 pKa = 11.84 DD34 pKa = 3.57 SSSGGLQIVNNMDD47 pKa = 3.23 TDD49 pKa = 4.02 VYY51 pKa = 11.16 LWTTSSDD58 pKa = 3.31 AGSMKK63 pKa = 10.11 TLSTGGDD70 pKa = 3.79 YY71 pKa = 11.37 SEE73 pKa = 5.77 DD74 pKa = 3.05 WATNSNGGGISIKK87 pKa = 10.18 MSTSEE92 pKa = 4.29 SKK94 pKa = 11.07 DD95 pKa = 3.2 SVLQFEE101 pKa = 4.64 YY102 pKa = 10.25 TQDD105 pKa = 3.43 GDD107 pKa = 4.04 TLFWDD112 pKa = 4.11 MSSIDD117 pKa = 4.43 LDD119 pKa = 3.86 STSEE123 pKa = 4.0 FVKK126 pKa = 10.8 SGFTVVPSDD135 pKa = 4.0 SSCKK139 pKa = 10.14 SVSCAAGDD147 pKa = 4.17 AEE149 pKa = 5.81 CKK151 pKa = 10.0 DD152 pKa = 4.38 AYY154 pKa = 10.57 QLPDD158 pKa = 3.43 DD159 pKa = 4.56 VNTYY163 pKa = 10.49 SCSLSSGFTLTLGG176 pKa = 3.59
Molecular weight: 18.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.795
IPC_protein 3.795
Toseland 3.567
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.63
EMBOSS 3.77
Sillero 3.923
Patrickios 1.901
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|A0A0M8P0P1|A0A0M8P0P1_9EURO Non-specific serine/threonine protein kinase OS=Penicillium nordicum OX=229535 GN=ACN38_g10971 PE=4 SV=1
MM1 pKa = 7.49 PVSPSRR7 pKa = 11.84 TRR9 pKa = 11.84 PSTAGRR15 pKa = 11.84 TMSTTTSVPLPRR27 pKa = 11.84 ARR29 pKa = 11.84 TSVPASSSTTPSALFAPRR47 pKa = 11.84 PGPIVGTVSARR58 pKa = 11.84 LATSLSTLTSRR69 pKa = 5.13
Molecular weight: 7.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.106
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12938
0
12938
5328948
8
6304
411.9
45.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.189 ± 0.021
1.283 ± 0.009
5.596 ± 0.018
6.023 ± 0.026
3.872 ± 0.015
6.713 ± 0.02
2.473 ± 0.01
5.168 ± 0.018
4.649 ± 0.02
9.037 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.272 ± 0.008
3.762 ± 0.012
6.072 ± 0.025
4.048 ± 0.018
5.961 ± 0.02
8.449 ± 0.024
6.021 ± 0.014
6.125 ± 0.016
1.469 ± 0.009
2.814 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here