Mycobacterium virus Joedirt

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronvirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1BQN2|G1BQN2_9CAUD Uncharacterized protein OS=Mycobacterium virus Joedirt OX=1034137 GN=105 PE=4 SV=1
MM1 pKa = 7.88IEE3 pKa = 4.38VYY5 pKa = 10.65NSDD8 pKa = 2.83MDD10 pKa = 4.13ARR12 pKa = 11.84CSVCDD17 pKa = 3.77DD18 pKa = 4.02MIRR21 pKa = 11.84RR22 pKa = 11.84GDD24 pKa = 3.76EE25 pKa = 3.9VVEE28 pKa = 4.06FAGEE32 pKa = 4.09LFHH35 pKa = 7.43GEE37 pKa = 4.3CAA39 pKa = 3.86

Molecular weight: 4.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1BQG6|G1BQG6_9CAUD Uncharacterized protein OS=Mycobacterium virus Joedirt OX=1034137 GN=37 PE=4 SV=1
MM1 pKa = 7.09HH2 pKa = 6.81TTHH5 pKa = 6.51SVLYY9 pKa = 8.25HH10 pKa = 4.51TQIVDD15 pKa = 3.5SALLGTVLAAGCTALYY31 pKa = 9.82IRR33 pKa = 11.84RR34 pKa = 11.84HH35 pKa = 4.23TWLVRR40 pKa = 11.84WEE42 pKa = 4.23TAATACIALLCVGTLCLSPQLIVFNPRR69 pKa = 11.84LSPEE73 pKa = 3.64IRR75 pKa = 11.84TDD77 pKa = 3.41ALPGQFLIGYY87 pKa = 7.59LTLLLAAQMFLLDD100 pKa = 3.61LAGRR104 pKa = 11.84MVWEE108 pKa = 4.68CAAKK112 pKa = 9.92ARR114 pKa = 11.84YY115 pKa = 7.76MNLYY119 pKa = 9.51VVLPGTIMLPTVIGLWTAQSRR140 pKa = 11.84WVMEE144 pKa = 3.81ILAVSLIYY152 pKa = 9.91TLSHH156 pKa = 6.2IGYY159 pKa = 9.01LLWRR163 pKa = 11.84IKK165 pKa = 10.14RR166 pKa = 11.84TDD168 pKa = 3.37PRR170 pKa = 11.84SGIVVDD176 pKa = 3.59LHH178 pKa = 6.28LAAFVFQVAAPLTRR192 pKa = 11.84WFAPVSLWEE201 pKa = 3.8PWGWRR206 pKa = 11.84FRR208 pKa = 11.84SFAALLFMAAASISWIRR225 pKa = 11.84KK226 pKa = 6.94RR227 pKa = 11.84RR228 pKa = 11.84YY229 pKa = 9.09IKK231 pKa = 10.11LHH233 pKa = 3.83MWQRR237 pKa = 11.84IRR239 pKa = 11.84AEE241 pKa = 4.08KK242 pKa = 9.08PLKK245 pKa = 10.29RR246 pKa = 11.84KK247 pKa = 7.19TSRR250 pKa = 11.84RR251 pKa = 11.84EE252 pKa = 3.66HH253 pKa = 6.27RR254 pKa = 11.84RR255 pKa = 11.84HH256 pKa = 5.37KK257 pKa = 9.55TPHH260 pKa = 5.49

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

126

0

126

22757

32

1643

180.6

19.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.366 ± 0.334

1.085 ± 0.13

6.249 ± 0.168

6.424 ± 0.293

3.133 ± 0.116

8.2 ± 0.393

2.017 ± 0.131

4.298 ± 0.15

5.093 ± 0.246

8.578 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.139

3.748 ± 0.176

5.124 ± 0.162

3.366 ± 0.171

6.543 ± 0.265

5.348 ± 0.212

5.651 ± 0.259

7.154 ± 0.218

2.122 ± 0.149

3.146 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski