Bombyx mandarina nucleopolyhedrovirus
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 141 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3VNV1|C3VNV1_NPVBM VP1054 OS=Bombyx mandarina nucleopolyhedrovirus OX=640862 PE=4 SV=1
MM1 pKa = 7.0 TNVWSAADD9 pKa = 3.56 VNLINCVLKK18 pKa = 11.16 DD19 pKa = 3.72 NLFLINNNYY28 pKa = 9.84 IILNVFDD35 pKa = 4.36 QEE37 pKa = 4.17 TDD39 pKa = 3.26 QVRR42 pKa = 11.84 PLCLGEE48 pKa = 4.21 INAFQTDD55 pKa = 3.22 AAAQADD61 pKa = 3.89 AMLDD65 pKa = 3.42 TSSTSEE71 pKa = 4.07 LQSNASTT78 pKa = 3.69
Molecular weight: 8.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.873
IPC2_protein 3.554
IPC_protein 3.452
Toseland 3.261
ProMoST 3.694
Dawson 3.49
Bjellqvist 3.656
Wikipedia 3.49
Rodwell 3.3
Grimsley 3.185
Solomon 3.414
Lehninger 3.376
Nozaki 3.643
DTASelect 3.834
Thurlkill 3.363
EMBOSS 3.49
Sillero 3.592
Patrickios 1.812
IPC_peptide 3.414
IPC2_peptide 3.541
IPC2.peptide.svr19 3.663
Protein with the highest isoelectric point:
>tr|C3VNU2|C3VNU2_NPVBM Ac44-like protein OS=Bombyx mandarina nucleopolyhedrovirus OX=640862 PE=4 SV=1
MM1 pKa = 6.3 NTRR4 pKa = 11.84 YY5 pKa = 7.93 ATCYY9 pKa = 9.91 VCDD12 pKa = 3.58 EE13 pKa = 4.24 LVYY16 pKa = 10.75 LFKK19 pKa = 10.53 KK20 pKa = 8.59 TFSNMSPSAAAFYY33 pKa = 7.6 QQRR36 pKa = 11.84 MAIVKK41 pKa = 10.1 NGIVLCVRR49 pKa = 11.84 CSSKK53 pKa = 11.02 LKK55 pKa = 9.96 IGNGISIPIYY65 pKa = 8.82 PNRR68 pKa = 11.84 RR69 pKa = 11.84 AQQHH73 pKa = 4.95 ARR75 pKa = 11.84 RR76 pKa = 11.84 SRR78 pKa = 3.12
Molecular weight: 8.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.78
IPC_protein 10.306
Toseland 10.233
ProMoST 10.175
Dawson 10.452
Bjellqvist 10.204
Wikipedia 10.657
Rodwell 10.73
Grimsley 10.54
Solomon 10.496
Lehninger 10.452
Nozaki 10.306
DTASelect 10.175
Thurlkill 10.292
EMBOSS 10.643
Sillero 10.379
Patrickios 10.423
IPC_peptide 10.496
IPC2_peptide 9.428
IPC2.peptide.svr19 8.445
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
141
0
141
38425
53
1222
272.5
31.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.195 ± 0.187
2.392 ± 0.146
6.072 ± 0.149
5.327 ± 0.158
4.937 ± 0.125
3.323 ± 0.194
2.209 ± 0.1
6.576 ± 0.105
7.196 ± 0.239
9.286 ± 0.149
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.774 ± 0.087
8.234 ± 0.179
3.844 ± 0.197
3.724 ± 0.173
4.721 ± 0.18
6.23 ± 0.16
5.819 ± 0.15
6.605 ± 0.13
0.747 ± 0.067
4.789 ± 0.106
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here