Corynebacterium provencense
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2661 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z3YNI0|A0A2Z3YNI0_9CORY Putative N-succinyldiaminopimelate aminotransferase DapC OS=Corynebacterium provencense OX=1737425 GN=dapC PE=4 SV=1
MM1 pKa = 7.62 PRR3 pKa = 11.84 RR4 pKa = 11.84 SAVPTTAMSMTAVVLALLTGCSGEE28 pKa = 4.91 DD29 pKa = 3.38 PAASPTFTAAEE40 pKa = 4.33 TTSDD44 pKa = 3.39 NTGGDD49 pKa = 3.19 TGGWDD54 pKa = 3.29 STGPQPSATSDD65 pKa = 3.2 TVAADD70 pKa = 3.61 AVSGTTTVTPQSGADD85 pKa = 3.61 APSSVPGADD94 pKa = 3.18 RR95 pKa = 11.84 PASLTEE101 pKa = 4.31 SGWSGSSVRR110 pKa = 11.84 CNTGDD115 pKa = 3.12 PWVYY119 pKa = 10.36 AAEE122 pKa = 4.44 GAPGQVVICLDD133 pKa = 4.07 DD134 pKa = 4.62 DD135 pKa = 3.81 ALYY138 pKa = 10.72 AVDD141 pKa = 3.99 TDD143 pKa = 3.65 NPYY146 pKa = 11.24 GGDD149 pKa = 3.22 NAYY152 pKa = 10.36 AATSAVCPVSGTGRR166 pKa = 11.84 DD167 pKa = 3.19 RR168 pKa = 11.84 YY169 pKa = 9.42 VWHH172 pKa = 6.94 HH173 pKa = 7.15 ADD175 pKa = 3.83 TGWTEE180 pKa = 3.83 YY181 pKa = 11.05 DD182 pKa = 3.0 GTTRR186 pKa = 11.84 YY187 pKa = 10.79 VMDD190 pKa = 3.91 IFSGRR195 pKa = 11.84 GRR197 pKa = 11.84 PDD199 pKa = 2.75 LTDD202 pKa = 3.28 AANIVEE208 pKa = 4.35 DD209 pKa = 4.95 AYY211 pKa = 11.19 DD212 pKa = 4.1 GAWTNPDD219 pKa = 3.19 VPVPTLRR226 pKa = 11.84 WCRR229 pKa = 11.84 HH230 pKa = 5.73 AGDD233 pKa = 4.21 SPSTNLLAGG242 pKa = 3.81
Molecular weight: 24.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.859
IPC_protein 3.884
Toseland 3.643
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.872
Rodwell 3.706
Grimsley 3.554
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.317
Thurlkill 3.719
EMBOSS 3.872
Sillero 4.012
Patrickios 1.163
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.883
Protein with the highest isoelectric point:
>tr|A0A2Z3YLS0|A0A2Z3YLS0_9CORY Catalase OS=Corynebacterium provencense OX=1737425 GN=katA PE=3 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AHH17 pKa = 5.01 KK18 pKa = 10.01 HH19 pKa = 3.72 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 SKK40 pKa = 10.67 GRR42 pKa = 11.84 KK43 pKa = 9.06 SLTAA47 pKa = 4.07
Molecular weight: 5.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2661
0
2661
922959
30
3136
346.8
37.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.902 ± 0.055
0.738 ± 0.014
6.709 ± 0.042
5.428 ± 0.042
2.921 ± 0.029
9.679 ± 0.045
2.068 ± 0.023
3.831 ± 0.034
1.971 ± 0.035
9.475 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.912 ± 0.021
2.119 ± 0.023
5.639 ± 0.036
2.567 ± 0.023
7.466 ± 0.051
5.986 ± 0.036
6.897 ± 0.039
9.357 ± 0.044
1.378 ± 0.019
1.957 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here